山黄柏提取物可在体外和体内抑制肺癌细胞的增殖和侵袭。

IF 2 4区 医学 Q3 NUTRITION & DIETETICS
Nutrition Research and Practice Pub Date : 2023-12-01 Epub Date: 2023-11-08 DOI:10.4162/nrp.2023.17.6.1070
Weike Wang, Jiling Song, Na Lu, Jing Yan, Guanping Chen
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引用次数: 0

摘要

背景/目的:桑黄菌(SS)具有多种药用功效,包括抗炎和抗癌活性。尽管对桑黄菌进行了广泛的研究,但其对肺癌的分子作用机制尚不清楚。本研究采用体外和体内模型研究了三黄醇提取物(SAE)对肺癌的影响:材料/方法:使用不同浓度的SAE培养肺癌细胞(A549和H1650)。采用细胞计数试剂盒-8 检测 A549 和 H1650 细胞的存活能力。划痕试验和 transwell 细胞侵袭试验用于检测 SAE 的迁移率和侵袭能力。用 Western 印迹分析检测 B 细胞淋巴瘤-2(Bcl-2)、Bcl2 相关 X(Bax)、细胞周期蛋白 D1、细胞周期蛋白依赖性激酶 4(CDK4)、信号转导和激活转录 3(STAT3)以及磷酸化 STAT3(p-STAT3)的表达。用肺癌异种移植小鼠来检测 SAE 在体内的抑制能力。结果显示,SAE对肺癌异种移植小鼠的肿瘤结构变化以及Bcl-2、Bax、细胞周期蛋白D1、CDK4、STAT3和p-STAT3的表达均有抑制作用:结果:SAE能显著抑制肺癌在体外和体内的增殖,且无细胞毒性。SAE对肺癌细胞活力、迁移和侵袭的抑制作用呈剂量和时间依赖性。在体外和体内,SAE能明显降低促凋亡的Bcl-2/Bax比率以及促增殖蛋白Cyclin D1和CDK4的表达。此外,SAE 还抑制了 STAT3 的表达:结论:SAE 可降低人肺癌细胞的存活率,抑制细胞迁移和侵袭。结论:SAE 可降低人肺癌细胞的存活率,抑制细胞迁移和侵袭,而且在体内也有抗增殖作用。因此,SAE 可用于癌症治疗。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Sanghuangporus sanghuang extract inhibits the proliferation and invasion of lung cancer cells in vitro and in vivo.

Background/objectives: Sanghuangporus sanghuang (SS) has various medicinal effects, including anti-inflammation and anticancer activities. Despite the extensive research on SS, its molecular mechanisms of action on lung cancer are unclear. This study examined the impact of an SS alcohol extract (SAE) on lung cancer using in vitro and in vivo models.

Materials/methods: Different concentrations of SAE were used to culture lung cancer cells (A549 and H1650). A cell counting kit-8 assay was used to detect the survival ability of A549 and H1650 cells. A scratch assay and transwell cell invasion assay were used to detect the migration rate and invasive ability of SAE. Western blot analysis was used to detect the expression of B-cell lymphoma-2 (Bcl-2), Bcl2-associated X (Bax), cyclin D1, cyclin-dependent kinases 4 (CDK4), signal transducer and activator of transcription 3 (STAT3), and phosphorylated STAT3 (p-STAT3). Lung cancer xenograft mice were used to detect the inhibiting ability of SAE in vivo. Hematoxylin and eosin staining and immunohistochemistry were used to detect the effect of SAE on the structural changes to the tumor and the expression of Bcl-2, Bax, cyclin D1, CDK4, STAT3, and p-STAT3 in lung cancer xenograft mice.

Results: SAE could inhibit lung cancer proliferation significantly in vitro and in vivo without cytotoxicity. SAE suppressed the viability, migration, and invasion of lung cancer cells in a dose and time-dependent manner. The SAE treatment significantly decreased the proapoptotic Bcl-2/Bax ratio and the expression of pro-proliferative proteins Cyclin D1 and CDK4 in vitro and in vivo. Furthermore, SAE also inhibited STAT3 expression.

Conclusions: SAE reduced the cell viability and suppressed cell migration and invasion in human lung cancer cells. Moreover, SAE also exhibited anti-proliferation effects in vivo. Therefore, SAE may have benefits in cancer therapy.

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来源期刊
Nutrition Research and Practice
Nutrition Research and Practice NUTRITION & DIETETICS-
CiteScore
3.50
自引率
4.20%
发文量
62
审稿时长
6-12 weeks
期刊介绍: Nutrition Research and Practice (NRP) is an official journal, jointly published by the Korean Nutrition Society and the Korean Society of Community Nutrition since 2007. The journal had been published quarterly at the initial stage and has been published bimonthly since 2010. NRP aims to stimulate research and practice across diverse areas of human nutrition. The Journal publishes peer-reviewed original manuscripts on nutrition biochemistry and metabolism, community nutrition, nutrition and disease management, nutritional epidemiology, nutrition education, foodservice management in the following categories: Original Research Articles, Notes, Communications, and Reviews. Reviews will be received by the invitation of the editors only. Statements made and opinions expressed in the manuscripts published in this Journal represent the views of authors and do not necessarily reflect the opinion of the Societies.
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