Cydonia oblonga Miller 果实提取物通过激活 AMPK 信号通路,在 3T3-L1 脂肪细胞中发挥抗肥胖作用。

IF 2 4区 医学 Q3 NUTRITION & DIETETICS
Nutrition Research and Practice Pub Date : 2023-12-01 Epub Date: 2023-08-21 DOI:10.4162/nrp.2023.17.6.1043
Hyun Sook Lee, Jae In Jung, Jung Soon Hwang, Myeong Oh Hwang, Eun Ji Kim
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引用次数: 0

摘要

背景/目的:在地中海地区的传统医学中,Cydonia oblonga Miller(COM)的果实被用于预防或治疗肥胖症,但其作用机制尚不清楚。材料/方法:用不同浓度(0-600 µg/mL)的COM 果实提取物(COME)和脂肪细胞分化培养基培养 3T3-L1 前脂肪细胞 8 天。用逆转录聚合酶链反应和 Western 印迹法分别分析了脂肪生成相关基因的表达水平和蛋白表达:结果:COME抑制了脂肪生成过程中细胞内TG的积累。3T3-L1细胞经COME处理后,单磷酸腺苷激活蛋白激酶(AMPK)α磷酸化上调,固醇调节元件结合蛋白1c、过氧化物酶体增殖激活受体γ和CCAAT/增强子结合蛋白α等致脂转录因子下调。COME 处理降低了脂肪酰基合成酶、三磷酸腺苷柠檬酸裂解酶、脂肪细胞蛋白 2 和脂蛋白脂肪酶的 mRNA 表达。结论:COME 能通过抑制脂肪细胞的脂肪生成,从而减少脂肪细胞中的脂肪酰合成酶、三磷酸腺苷柠檬酸酶、脂肪细胞蛋白 2 和脂蛋白脂肪酶的 mRNA 表达,增加 3T3-L1 细胞中激素敏感性脂肪酶和肉碱棕榈酰基转移酶 I 的 mRNA 表达:结论:COME 可通过 AMPK 信号通路抑制脂肪生成。结论:COME 通过 AMPK 信号通路抑制脂肪生成,可用于预防和治疗肥胖症。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cydonia oblonga Miller fruit extract exerts an anti-obesity effect in 3T3-L1 adipocytes by activating the AMPK signaling pathway.

Background/objectives: The fruit of Cydonia oblonga Miller (COM) is used traditionally in Mediterranean region medicine to prevent or treat obesity, but its mechanism of action is still unclear. Beyond a demonstrated anti-obesity effect, the fruit was tested for the mechanism of adipogenesis in 3T3-L1 preadipocytes.

Materials/methods: 3T3-L1 preadipocytes were cultured for 8 days with COM fruit extract (COME) at different concentrations (0-600 µg/mL) with adipocyte differentiation medium. The cell viability was measured using an MTT assay; triglyceride (TG) was stained with Oil Red O. The expression levels of the adipogenesis-related genes and protein expression were analyzed by reverse transcription polymerase chain reaction and Western blotting, respectively.

Results: COME inhibited intracellular TG accumulation during adipogenesis. A COME treatment in 3T3-L1 cells induced upregulation of the adenosine monophosphate-activated protein kinase (AMPK)α phosphorylation and downregulation of the adipogenic transcription factors, such as sterol regulatory element-binding protein 1c, peroxisome proliferator-activated receptor γ, and CCAAT/enhancer binding protein α. The COME treatment reduced the mRNA expression of fatty acyl synthetase, adenosine triphosphate-citrate lyase, adipocyte protein 2, and lipoprotein lipase. It increased the mRNA expression of hormone-sensitive lipase and carnitine palmitoyltransferase I in 3T3-L1 cells.

Conclusions: COME inhibits adipogenesis via the AMPK signaling pathways. COME may be used to prevent and treat obesity.

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来源期刊
Nutrition Research and Practice
Nutrition Research and Practice NUTRITION & DIETETICS-
CiteScore
3.50
自引率
4.20%
发文量
62
审稿时长
6-12 weeks
期刊介绍: Nutrition Research and Practice (NRP) is an official journal, jointly published by the Korean Nutrition Society and the Korean Society of Community Nutrition since 2007. The journal had been published quarterly at the initial stage and has been published bimonthly since 2010. NRP aims to stimulate research and practice across diverse areas of human nutrition. The Journal publishes peer-reviewed original manuscripts on nutrition biochemistry and metabolism, community nutrition, nutrition and disease management, nutritional epidemiology, nutrition education, foodservice management in the following categories: Original Research Articles, Notes, Communications, and Reviews. Reviews will be received by the invitation of the editors only. Statements made and opinions expressed in the manuscripts published in this Journal represent the views of authors and do not necessarily reflect the opinion of the Societies.
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