仿生血管化脂肪间充质干细胞骨-骨膜移植可增强雄性兔脊柱融合模型的血管生成和骨生成。

IF 4.7 2区 医学 Q2 CELL & TISSUE ENGINEERING
Tsai-Sheng Fu, Wei-Chuan Chen, Ying-Chih Wang, Chia-Wei Chang, Tung-Yi Lin, Chak-Bor Wong
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引用次数: 0

摘要

目的:目前已开发出多种人工骨移植物,但由于其血管新生能力和骨膜支持不足,无法实现预期的成骨效果。本研究旨在开发一种血管化骨骨膜构建体(VBPC),为骨再生提供更好的血管生成和成骨能力:方法:将 24 只雄性新西兰白兔按实验材料分为四组。方法:将 24 只雄性新西兰白兔按实验材料分为四组,分别培养异基因脂肪间充质干细胞(AMSCs)并将其均匀播种在胶原蛋白/壳聚糖薄片上,形成细胞薄片作为骨膜。同时,将异源 AMSCs 接种到藻酸盐珠上,培养分化为内皮样细胞,形成血管化骨构建物(VBC)。将细胞薄片包裹在 VBC 上,形成血管化骨-骨膜构建体(VBPC)。然后将四种不同的实验材料--无细胞构建物、VBC、无血管化骨-骨膜构建物和 VBPC--植入双侧 L4-L5 横向间隙。术后 12 周,通过 CT、生物力学测试、组织学和免疫组化染色分析确定骨形成能力:结果:12 周时,VBPC 组与其他组相比,新骨形成量明显增加。生物力学测试表明,VBPC 组的扭矩强度更高。值得注意的是,血红素和伊红、Masson 三色和免疫组化染色的组织学结果显示,VBPC 促进了脊柱融合区域的新生血管形成和新骨形成:结论:组织工程 VBPC 在促进体内血管生成和骨生成方面表现出了强大的能力。结论:组织工程 VBPC 在促进体内血管生成和骨生成方面表现出很强的能力,可为创造良好的血液供应和营养环境提供一种新方法,以克服当前人工骨移植替代物的不足。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Biomimetic vascularized adipose-derived mesenchymal stem cells bone-periosteum graft enhances angiogenesis and osteogenesis in a male rabbit spine fusion model.

Aims: Several artificial bone grafts have been developed but fail to achieve anticipated osteogenesis due to their insufficient neovascularization capacity and periosteum support. This study aimed to develop a vascularized bone-periosteum construct (VBPC) to provide better angiogenesis and osteogenesis for bone regeneration.

Methods: A total of 24 male New Zealand white rabbits were divided into four groups according to the experimental materials. Allogenic adipose-derived mesenchymal stem cells (AMSCs) were cultured and seeded evenly in the collagen/chitosan sheet to form cell sheet as periosteum. Simultaneously, allogenic AMSCs were seeded onto alginate beads and were cultured to differentiate to endothelial-like cells to form vascularized bone construct (VBC). The cell sheet was wrapped onto VBC to create a vascularized bone-periosteum construct (VBPC). Four different experimental materials - acellular construct, VBC, non-vascularized bone-periosteum construct, and VBPC - were then implanted in bilateral L4-L5 intertransverse space. At 12 weeks post-surgery, the bone-forming capacities were determined by CT, biomechanical testing, histology, and immunohistochemistry staining analyses.

Results: At 12 weeks, the VBPC group significantly increased new bone formation volume compared with the other groups. Biomechanical testing demonstrated higher torque strength in the VBPC group. Notably, the haematoxylin and eosin, Masson's trichrome, and immunohistochemistry-stained histological results revealed that VBPC promoted neovascularization and new bone formation in the spine fusion areas.

Conclusion: The tissue-engineered VBPC showed great capability in promoting angiogenesis and osteogenesis in vivo. It may provide a novel approach to create a superior blood supply and nutritional environment to overcome the deficits of current artificial bone graft substitutes.

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来源期刊
Bone & Joint Research
Bone & Joint Research CELL & TISSUE ENGINEERING-ORTHOPEDICS
CiteScore
7.40
自引率
23.90%
发文量
156
审稿时长
12 weeks
期刊介绍: The gold open access journal for the musculoskeletal sciences. Included in PubMed and available in PubMed Central.
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