AMP 脱氨酶:Mucor circinelloides 中氮胁迫和脂质代谢的关键调节因子。

IF 3.9 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Shaoqi Li , Junhuan Yang , Hassan Mohamed , Xiuwen Wang , Wenyue Shi , Futing Xue , Sergio López-García , Qing Liu , Yuanda Song
{"title":"AMP 脱氨酶:Mucor circinelloides 中氮胁迫和脂质代谢的关键调节因子。","authors":"Shaoqi Li ,&nbsp;Junhuan Yang ,&nbsp;Hassan Mohamed ,&nbsp;Xiuwen Wang ,&nbsp;Wenyue Shi ,&nbsp;Futing Xue ,&nbsp;Sergio López-García ,&nbsp;Qing Liu ,&nbsp;Yuanda Song","doi":"10.1016/j.bbalip.2023.159434","DOIUrl":null,"url":null,"abstract":"<div><p><span>Lipid biosynthesis is a significant metabolic response to nitrogen starvation in oleaginous fungi. The oleaginous fungus </span><span><em>Mucor circinelloides</em></span><span><span> copes with nitrogen stress by degrading AMP through AMP deaminase (AMPD). However, the mechanism of AMPD in regulating lipogenesis remains largely unclear. To elucidate the mechanism of AMPD in </span>lipid synthesis in this </span><em>M. circinelloides</em>, we identified two genes (<em>ampd1</em> and <em>ampd2</em>) encoding AMPD and constructed an <em>ampd</em> double knockout mutant. The engineered <em>M. circinelloides</em><span><span><span> strain elevated cell growth and lipid accumulation, as well as the content of </span>oleic acid (OA) and gamma-linolenic acid (GLA). In addition to the expected increase in transcription levels of genes associated with lipid and </span>TAG<span><span><span> synthesis, we observed suppression of lipid degradation<span> and reduced amino acid biosynthesis. This suggested that the deletion of AMPD genes induces the redirection of carbon towards lipid synthesis pathways. Moreover, the pathways related to </span></span>nitrogen metabolism, including </span>nitrogen assimilation<span> and purine metabolism<span> (especially energy level), were also affected in order to maintain homeostasis. Further analysis discovered that the transcription factors (TFs) related to lipid accumulation were also regulated. This study provides new insights into lipid biosynthesis in </span></span></span></span><em>M. circinelloides</em>, indicating that the trigger for lipid accumulation is not entirely AMPD-dependent and suggest that there may be additional mechanisms involved in the initiation of lipogenesis.</p></div>","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. Molecular and cell biology of lipids","volume":"1869 2","pages":"Article 159434"},"PeriodicalIF":3.9000,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"AMP deaminase: A crucial regulator in nitrogen stress and lipid metabolism in Mucor circinelloides\",\"authors\":\"Shaoqi Li ,&nbsp;Junhuan Yang ,&nbsp;Hassan Mohamed ,&nbsp;Xiuwen Wang ,&nbsp;Wenyue Shi ,&nbsp;Futing Xue ,&nbsp;Sergio López-García ,&nbsp;Qing Liu ,&nbsp;Yuanda Song\",\"doi\":\"10.1016/j.bbalip.2023.159434\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span>Lipid biosynthesis is a significant metabolic response to nitrogen starvation in oleaginous fungi. The oleaginous fungus </span><span><em>Mucor circinelloides</em></span><span><span> copes with nitrogen stress by degrading AMP through AMP deaminase (AMPD). However, the mechanism of AMPD in regulating lipogenesis remains largely unclear. To elucidate the mechanism of AMPD in </span>lipid synthesis in this </span><em>M. circinelloides</em>, we identified two genes (<em>ampd1</em> and <em>ampd2</em>) encoding AMPD and constructed an <em>ampd</em> double knockout mutant. The engineered <em>M. circinelloides</em><span><span><span> strain elevated cell growth and lipid accumulation, as well as the content of </span>oleic acid (OA) and gamma-linolenic acid (GLA). In addition to the expected increase in transcription levels of genes associated with lipid and </span>TAG<span><span><span> synthesis, we observed suppression of lipid degradation<span> and reduced amino acid biosynthesis. This suggested that the deletion of AMPD genes induces the redirection of carbon towards lipid synthesis pathways. Moreover, the pathways related to </span></span>nitrogen metabolism, including </span>nitrogen assimilation<span> and purine metabolism<span> (especially energy level), were also affected in order to maintain homeostasis. Further analysis discovered that the transcription factors (TFs) related to lipid accumulation were also regulated. This study provides new insights into lipid biosynthesis in </span></span></span></span><em>M. circinelloides</em>, indicating that the trigger for lipid accumulation is not entirely AMPD-dependent and suggest that there may be additional mechanisms involved in the initiation of lipogenesis.</p></div>\",\"PeriodicalId\":8815,\"journal\":{\"name\":\"Biochimica et biophysica acta. Molecular and cell biology of lipids\",\"volume\":\"1869 2\",\"pages\":\"Article 159434\"},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2023-12-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et biophysica acta. Molecular and cell biology of lipids\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1388198123001580\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et biophysica acta. Molecular and cell biology of lipids","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1388198123001580","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

脂质生物合成是油脂真菌对氮饥饿的一种重要代谢反应。油脂真菌 Mucor circinelloides 通过 AMPD(AMP脱氨酶)降解 AMP 来应对氮胁迫。 然而,AMPD 在调节脂质合成中的作用机制仍不清楚。为了阐明 AMPD 在环毛蚓脂质合成中的作用机制,我们确定了两个编码 AMPD 的基因(ampd1 和 ampd2),并构建了一个 ampd 双基因敲除突变体。改造后的环带褐藻菌株提高了细胞生长和脂质积累,以及油酸(OA)和γ-亚麻酸(GLA)的含量。除了与脂质和 TAG 合成相关的基因转录水平的预期增加外,我们还观察到脂质降解受到抑制,氨基酸生物合成减少。这表明 AMPD 基因的缺失诱导了碳向脂质合成途径的重新定向。此外,与氮代谢相关的途径,包括氮同化和嘌呤代谢(尤其是能量水平)也受到影响,以维持体内平衡。进一步分析发现,与脂质积累相关的转录因子(TFs)也受到了调控。这项研究提供了对环带藻脂质生物合成的新见解,表明脂质积累的触发因素并不完全依赖于 AMPD,并提示脂质生成的启动可能还涉及其他机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

AMP deaminase: A crucial regulator in nitrogen stress and lipid metabolism in Mucor circinelloides

AMP deaminase: A crucial regulator in nitrogen stress and lipid metabolism in Mucor circinelloides

AMP deaminase: A crucial regulator in nitrogen stress and lipid metabolism in Mucor circinelloides

Lipid biosynthesis is a significant metabolic response to nitrogen starvation in oleaginous fungi. The oleaginous fungus Mucor circinelloides copes with nitrogen stress by degrading AMP through AMP deaminase (AMPD). However, the mechanism of AMPD in regulating lipogenesis remains largely unclear. To elucidate the mechanism of AMPD in lipid synthesis in this M. circinelloides, we identified two genes (ampd1 and ampd2) encoding AMPD and constructed an ampd double knockout mutant. The engineered M. circinelloides strain elevated cell growth and lipid accumulation, as well as the content of oleic acid (OA) and gamma-linolenic acid (GLA). In addition to the expected increase in transcription levels of genes associated with lipid and TAG synthesis, we observed suppression of lipid degradation and reduced amino acid biosynthesis. This suggested that the deletion of AMPD genes induces the redirection of carbon towards lipid synthesis pathways. Moreover, the pathways related to nitrogen metabolism, including nitrogen assimilation and purine metabolism (especially energy level), were also affected in order to maintain homeostasis. Further analysis discovered that the transcription factors (TFs) related to lipid accumulation were also regulated. This study provides new insights into lipid biosynthesis in M. circinelloides, indicating that the trigger for lipid accumulation is not entirely AMPD-dependent and suggest that there may be additional mechanisms involved in the initiation of lipogenesis.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
11.00
自引率
2.10%
发文量
109
审稿时长
53 days
期刊介绍: BBA Molecular and Cell Biology of Lipids publishes papers on original research dealing with novel aspects of molecular genetics related to the lipidome, the biosynthesis of lipids, the role of lipids in cells and whole organisms, the regulation of lipid metabolism and function, and lipidomics in all organisms. Manuscripts should significantly advance the understanding of the molecular mechanisms underlying biological processes in which lipids are involved. Papers detailing novel methodology must report significant biochemical, molecular, or functional insight in the area of lipids.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信