Jacqueline M Serrano, Rachel Cook, Heather Headrick, W Rodney Cooper
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In an effort to understand the feeding ecology of click beetles, we investigated the presence of plant DNA in the digestive tracts of Limonius californicus (Mann.), L. canus (LeConte), and L. infuscatus (Mots.) beetles collected in 3 different locations within central Washington. To examine dietary histories of beetles and wireworms, specimens were collected from natural habitats and high-throughput sequencing of the plant genes trnF and ITS was used to identify their dietary history. Results revealed that click beetles do feed on a large variety of plants, which included a large quantity of brassicaceous plants commonly found in areas surrounding wireworm infested plots. The identification of the dietary history of the click beetles allowed us to infer their landscape-scale movements thus providing a means to better understand their behavior.</p>","PeriodicalId":11751,"journal":{"name":"Environmental Entomology","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Dietary history of click beetles and wireworms in the genus Limonius (Coleoptera: Elateridae) revealed by molecular gut content analysis.\",\"authors\":\"Jacqueline M Serrano, Rachel Cook, Heather Headrick, W Rodney Cooper\",\"doi\":\"10.1093/ee/nvad114\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Wireworms, the larval stage of pest click beetle species (Coleoptera: Elateridae), are pests of many crops in North America including root vegetables and cereals. 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To examine dietary histories of beetles and wireworms, specimens were collected from natural habitats and high-throughput sequencing of the plant genes trnF and ITS was used to identify their dietary history. Results revealed that click beetles do feed on a large variety of plants, which included a large quantity of brassicaceous plants commonly found in areas surrounding wireworm infested plots. 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引用次数: 0
摘要
线虫是北美许多农作物的害虫,包括根茎类蔬菜和谷类作物。由于可用于管理线虫的有效杀虫剂越来越少,因此面临来自线虫压力的种植者有理由感到担忧。大多数关于扁虫的研究都集中在线虫阶段,这是造成损害的生命阶段。近年来,昆虫研究的重点已经转移到成虫阶段,包括鉴定化学成分和开发有效的陷阱。然而,关于点击甲虫的基本生物学,包括它们的摄食生态,还有很多有待发现。为了了解点击甲虫的摄食生态,我们调查了在华盛顿中部3个不同地点收集的加利福尼亚Limonius (Mann.), L. canus (LeConte)和L. infuscatus (Mots.)甲虫消化道中植物DNA的存在。为了研究甲虫和线虫的饮食史,我们从自然栖息地收集了标本,并利用植物基因trnF和ITS的高通量测序来确定它们的饮食史。结果显示,点击甲虫确实以各种各样的植物为食,其中包括大量常见于蠕虫出没区域周围的十字花科植物。对点击甲虫饮食历史的识别使我们能够推断它们在景观尺度上的运动,从而为更好地理解它们的行为提供了一种方法。
Dietary history of click beetles and wireworms in the genus Limonius (Coleoptera: Elateridae) revealed by molecular gut content analysis.
Wireworms, the larval stage of pest click beetle species (Coleoptera: Elateridae), are pests of many crops in North America including root vegetables and cereals. There is cause for concern amongst growers who are facing pressure from wireworms because there are a decreasing number of effective pesticides that can be used for wireworm management. Most research on pest elaterids has focused on the wireworm stage, which is the damage causing life stage. Recently, the focus in elaterid research has shifted to the adult click beetle stage, including identification of semiochemicals and development of effective traps. However, there is still a lot to be discovered about the basic biology of click beetles, including their feeding ecology. In an effort to understand the feeding ecology of click beetles, we investigated the presence of plant DNA in the digestive tracts of Limonius californicus (Mann.), L. canus (LeConte), and L. infuscatus (Mots.) beetles collected in 3 different locations within central Washington. To examine dietary histories of beetles and wireworms, specimens were collected from natural habitats and high-throughput sequencing of the plant genes trnF and ITS was used to identify their dietary history. Results revealed that click beetles do feed on a large variety of plants, which included a large quantity of brassicaceous plants commonly found in areas surrounding wireworm infested plots. The identification of the dietary history of the click beetles allowed us to infer their landscape-scale movements thus providing a means to better understand their behavior.
期刊介绍:
Environmental Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes reports on the interaction of insects with the biological, chemical, and physical aspects of their environment. In addition to research papers, Environmental Entomology publishes Reviews, interpretive articles in a Forum section, and Letters to the Editor.