合成纳米纤维支架上心血管细胞的自组织行为。

IF 6.6 3区 医学 Q1 ENGINEERING, BIOMEDICAL
Michael M Peters, Jackson K Brister, Edward M Tang, Felita W Zhang, Veronica M Lucian, Paul D Trackey, Zachary Bone, John F Zimmerman, Qianru Jin, F John Burpo, Kevin Kit Parker
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引用次数: 0

摘要

在组织和器官中,细胞外基质(ECM)有助于维持细胞间和细胞内的结构,维持定义生理稳态的结构-功能关系。将纤维支架和细胞结合形成工程组织是复制这些关系的一种手段。工程组织的纤维支架被设计成模拟ECM网络的拓扑结构和化学成分。在这里,我们询问在心脏中发现的细胞如何比较它们对齐细胞骨架和自组织的倾向,以响应纤维支架中的拓扑线索。我们研究了心肌细胞、瓣膜间质细胞和血管内皮细胞如何适应细胞外空间的细胞间和细胞内结构。我们使用聚焦旋转喷射旋转来制造排列的纤维支架,以模仿心脏肌肉、瓣膜和血管组织中天然ECM的长度尺度和三维(3D)性质。将具有代表性的心血管细胞类型植入到纤维支架上并浸润到纤维网络中。我们测量了不同细胞类型对细胞骨架排列的倾向,以响应具有不同水平各向异性的纤维支架。结果表明,在中等各向异性基质上的瓣膜间质细胞比心肌细胞和血管内皮细胞具有更高的细胞骨架排列倾向。然而,所有细胞类型在更极端(各向同性和高度各向异性)的纤维支架组织上显示出相似的排列水平。这些数据表明,在指示组织时空组织的信号层次中,ECM和细胞网络中的几何线索可能使细胞群体和人口统计学中的行为均匀化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Self-organizing behaviors of cardiovascular cells on synthetic nanofiber scaffolds.

In tissues and organs, the extracellular matrix (ECM) helps maintain inter- and intracellular architectures that sustain the structure-function relationships defining physiological homeostasis. Combining fiber scaffolds and cells to form engineered tissues is a means of replicating these relationships. Engineered tissues' fiber scaffolds are designed to mimic the topology and chemical composition of the ECM network. Here, we asked how cells found in the heart compare in their propensity to align their cytoskeleton and self-organize in response to topological cues in fibrous scaffolds. We studied cardiomyocytes, valvular interstitial cells, and vascular endothelial cells as they adapted their inter- and intracellular architectures to the extracellular space. We used focused rotary jet spinning to manufacture aligned fibrous scaffolds to mimic the length scale and three-dimensional (3D) nature of the native ECM in the muscular, valvular, and vascular tissues of the heart. The representative cardiovascular cell types were seeded onto fiber scaffolds and infiltrated the fibrous network. We measured different cell types' propensity for cytoskeletal alignment in response to fiber scaffolds with differing levels of anisotropy. The results indicated that valvular interstitial cells on moderately anisotropic substrates have a higher propensity for cytoskeletal alignment than cardiomyocytes and vascular endothelial cells. However, all cell types displayed similar levels of alignment on more extreme (isotropic and highly anisotropic) fiber scaffold organizations. These data suggest that in the hierarchy of signals that dictate the spatiotemporal organization of a tissue, geometric cues within the ECM and cellular networks may homogenize behaviors across cell populations and demographics.

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来源期刊
APL Bioengineering
APL Bioengineering ENGINEERING, BIOMEDICAL-
CiteScore
9.30
自引率
6.70%
发文量
39
审稿时长
19 weeks
期刊介绍: APL Bioengineering is devoted to research at the intersection of biology, physics, and engineering. The journal publishes high-impact manuscripts specific to the understanding and advancement of physics and engineering of biological systems. APL Bioengineering is the new home for the bioengineering and biomedical research communities. APL Bioengineering publishes original research articles, reviews, and perspectives. Topical coverage includes: -Biofabrication and Bioprinting -Biomedical Materials, Sensors, and Imaging -Engineered Living Systems -Cell and Tissue Engineering -Regenerative Medicine -Molecular, Cell, and Tissue Biomechanics -Systems Biology and Computational Biology
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