Brummer脂肪酶缺乏会影响传虫的脂质动员和运动,使传虫的蛋壳超微结构出现缺陷,从而影响传虫的繁殖。

IF 3.9 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Daniela Saar Arêdes , Thamara Rios , Luiz Fernando Carvalho-Kelly , Valdir Braz , Luciana O. Araripe , Rafaela V. Bruno , José Roberto Meyer-Fernandes , Isabela Ramos , Katia C. Gondim
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引用次数: 0

摘要

长角锥虫(Rhodnius prolixus)是一种食血昆虫,以大量而不频繁的血液为食,是引起恰加斯病的锥虫病媒。进食后,从血粉中提取的脂质以甘油三酯的形式储存在脂肪体中,在需要能量的条件下,脂质分解被吸收,其中第一步是由Brummer脂肪酶(Bmm)催化的,其在哺乳动物中的同源物是脂肪甘油三酯脂肪酶(ATGL)。在此,我们研究了Bmm在饥饿和摄食后成虫体内的作用。其基因(RhoprBmm)在分析的所有昆虫器官中均有表达,其在脂肪体中的转录水平不受营养状况的影响。rnai介导的RhoprBmm的下调导致饥饿期间脂肪体中的甘油三酯滞留,导致与对照雌性相比,脂滴更大,ATP水平更低。沉默的雌性表现出飞行能力和运动能力下降。当RhoprBmm基因敲除发生在血餐和昆虫进食之前时,雌性产卵较少,卵塌陷,孵化率低。它们的血淋巴中二酰甘油含量和卵黄蛋白原浓度降低。与对照卵相比,其卵的绒毛膜(蛋壳)的碳氢化合物含量和二酪氨酸交联水平没有差异。但在超微结构上存在缺陷。这些结果表明,Bmm活性不仅对保证饥饿期间脂质动员以维持能量稳态很重要,而且通过某种方式促进卵毛膜的正确形成,对血食后活卵的产生也很重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Deficiency of Brummer lipase disturbs lipid mobilization and locomotion, and impairs reproduction due to defects in the eggshell ultrastructure in the insect vector Rhodnius prolixus

Deficiency of Brummer lipase disturbs lipid mobilization and locomotion, and impairs reproduction due to defects in the eggshell ultrastructure in the insect vector Rhodnius prolixus

Rhodnius prolixus is a hematophagous insect, which feeds on large and infrequent blood meals, and is a vector of trypanosomatids that cause Chagas disease. After feeding, lipids derived from blood meal are stored in the fat body as triacylglycerol, which is recruited under conditions of energy demand by lipolysis, where the first step is catalyzed by the Brummer lipase (Bmm), whose orthologue in mammals is the adipose triglyceride lipase (ATGL). Here, we investigated the roles of Bmm in adult Rhodnius prolixus under starvation, and after feeding. Its gene (RhoprBmm) was expressed in all the analyzed insect organs, and its transcript levels in the fat body were not altered by nutritional status. RNAi-mediated knockdown of RhoprBmm caused triacylglycerol retention in the fat body during starvation, resulting in larger lipid droplets and lower ATP levels compared to control females. The silenced females showed decreased flight capacity and locomotor activity. When RhoprBmm knockdown occurred before the blood meal and the insects were fed, the females laid fewer eggs, which collapsed and showed low hatching rates. Their hemolymph had reduced diacylglycerol content and vitellogenin concentration. The chorion (eggshell) of their eggs had no difference in hydrocarbon amounts or in dityrosine crosslinking levels compared to control eggs. However, it showed ultrastructural defects. These results demonstrated that Bmm activity is important not only to guarantee lipid mobilization to maintain energy homeostasis during starvation, but also for the production of viable eggs after a blood meal, by somehow contributing to the right formation of the egg chorion.

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来源期刊
CiteScore
11.00
自引率
2.10%
发文量
109
审稿时长
53 days
期刊介绍: BBA Molecular and Cell Biology of Lipids publishes papers on original research dealing with novel aspects of molecular genetics related to the lipidome, the biosynthesis of lipids, the role of lipids in cells and whole organisms, the regulation of lipid metabolism and function, and lipidomics in all organisms. Manuscripts should significantly advance the understanding of the molecular mechanisms underlying biological processes in which lipids are involved. Papers detailing novel methodology must report significant biochemical, molecular, or functional insight in the area of lipids.
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