{"title":"生物合成纳米银修饰玻碳电极作为前列腺特异性抗原检测的电化学生物传感器","authors":"Naresh Mandal , Raja Mitra , Bidhan Pramanick","doi":"10.1016/j.cartre.2023.100315","DOIUrl":null,"url":null,"abstract":"<div><p>Prostate cancer is the second leading cause of death for men after lung cancer. Therefore, early detection of cancer proteins or biomarkers is crucial in assessing the disease's reappearance after treatment. Here, we have reported the glassy carbon electrode (GCE) based electrochemical biosensors fabricated using Carbon-Microelectromechanical Systems (C-MEMS) technology for prostate-specific antigen (PSA) quantification. This report also presents the biosensor improvement by modifying the GCE with silver nanoparticles (AgNPs). Moreover, our investigation extends to bio-synthesized AgNPs that bring together advantageous chemical and physical attributes at a reasonable cost. In addition, AgNPs modified GCE have a more comprehensive anodic potential range [˗0.2 V to +0.3 V] than bare glassy carbon (GC). Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) measurements were used to examine the electrochemical response of the biosensors. As a result of the use of AgNPs-modified GCE, we have developed a label-free analytical system for prostate cancer detection, which can provide a linear response in the concentration range of 1 pg/ml - 3 µg/ml in a reasonably simple operation. The developed biosensors may be used for the early detection of prostate cancer in clinical analysis due to the excellent sensitivity demonstrated by this electrochemical biosensor.</p></div>","PeriodicalId":52629,"journal":{"name":"Carbon Trends","volume":null,"pages":null},"PeriodicalIF":3.1000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667056923000706/pdfft?md5=5e55b6439788e686137bfd5061a75d32&pid=1-s2.0-S2667056923000706-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Bio-synthesized silver nanoparticle modified glassy carbon electrode as electrochemical biosensor for prostate specific antigen detection\",\"authors\":\"Naresh Mandal , Raja Mitra , Bidhan Pramanick\",\"doi\":\"10.1016/j.cartre.2023.100315\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Prostate cancer is the second leading cause of death for men after lung cancer. Therefore, early detection of cancer proteins or biomarkers is crucial in assessing the disease's reappearance after treatment. Here, we have reported the glassy carbon electrode (GCE) based electrochemical biosensors fabricated using Carbon-Microelectromechanical Systems (C-MEMS) technology for prostate-specific antigen (PSA) quantification. This report also presents the biosensor improvement by modifying the GCE with silver nanoparticles (AgNPs). Moreover, our investigation extends to bio-synthesized AgNPs that bring together advantageous chemical and physical attributes at a reasonable cost. In addition, AgNPs modified GCE have a more comprehensive anodic potential range [˗0.2 V to +0.3 V] than bare glassy carbon (GC). Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) measurements were used to examine the electrochemical response of the biosensors. As a result of the use of AgNPs-modified GCE, we have developed a label-free analytical system for prostate cancer detection, which can provide a linear response in the concentration range of 1 pg/ml - 3 µg/ml in a reasonably simple operation. The developed biosensors may be used for the early detection of prostate cancer in clinical analysis due to the excellent sensitivity demonstrated by this electrochemical biosensor.</p></div>\",\"PeriodicalId\":52629,\"journal\":{\"name\":\"Carbon Trends\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.1000,\"publicationDate\":\"2023-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2667056923000706/pdfft?md5=5e55b6439788e686137bfd5061a75d32&pid=1-s2.0-S2667056923000706-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Carbon Trends\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2667056923000706\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MATERIALS SCIENCE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Carbon Trends","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2667056923000706","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
摘要
前列腺癌是仅次于肺癌的男性第二大死因。因此,早期检测癌症蛋白或生物标志物对于评估治疗后疾病的复发至关重要。在这里,我们报道了利用碳-微机电系统(C-MEMS)技术制造的基于玻璃碳电极(GCE)的电化学生物传感器,用于前列腺特异性抗原(PSA)的定量。本文还介绍了用纳米银修饰GCE对生物传感器的改进。此外,我们的研究扩展到以合理的成本将有利的化学和物理特性结合在一起的生物合成AgNPs。此外,AgNPs修饰的GCE具有更广泛的阳极电位范围[0.2 V ~ +0.3 V],远高于裸玻碳。利用电化学阻抗谱(EIS)和循环伏安法(CV)检测了生物传感器的电化学响应。由于使用agnps修饰的GCE,我们开发了一种无标记的前列腺癌检测分析系统,该系统可以在1 pg/ml - 3µg/ml的浓度范围内提供线性响应,操作相当简单。所研制的电化学生物传感器具有良好的灵敏度,可用于前列腺癌的早期检测。
Bio-synthesized silver nanoparticle modified glassy carbon electrode as electrochemical biosensor for prostate specific antigen detection
Prostate cancer is the second leading cause of death for men after lung cancer. Therefore, early detection of cancer proteins or biomarkers is crucial in assessing the disease's reappearance after treatment. Here, we have reported the glassy carbon electrode (GCE) based electrochemical biosensors fabricated using Carbon-Microelectromechanical Systems (C-MEMS) technology for prostate-specific antigen (PSA) quantification. This report also presents the biosensor improvement by modifying the GCE with silver nanoparticles (AgNPs). Moreover, our investigation extends to bio-synthesized AgNPs that bring together advantageous chemical and physical attributes at a reasonable cost. In addition, AgNPs modified GCE have a more comprehensive anodic potential range [˗0.2 V to +0.3 V] than bare glassy carbon (GC). Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) measurements were used to examine the electrochemical response of the biosensors. As a result of the use of AgNPs-modified GCE, we have developed a label-free analytical system for prostate cancer detection, which can provide a linear response in the concentration range of 1 pg/ml - 3 µg/ml in a reasonably simple operation. The developed biosensors may be used for the early detection of prostate cancer in clinical analysis due to the excellent sensitivity demonstrated by this electrochemical biosensor.