ABHD5-CPT1B:调节妊娠期糖尿病胎盘脂质代谢的重要途径

IF 4.7 3区 医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL
Yuqi Yang , Yue Peng , Bin Yu , Huiyan Wang
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引用次数: 0

摘要

背景与目的妊娠期糖尿病(GDM)是妊娠期最常见的代谢性疾病之一,母体脂质谱与妊娠期糖尿病的发生有重要关系。然而,其分子机制尚不清楚。方法生物分析结合胎盘代谢组学和单细胞rna测序(scRNA-seq)成功鉴定出一个潜在的重要分子:α-β水解酶结构域蛋白5 (ABHD5)。采用合胞滋养细胞(SCT)模型作为BeWo细胞对forskolin的反应。在此基础上,进行高糖刺激细胞实验。招募15名GDM妇女和15名正常孕妇进行验证实验。结果abhd5主要在滋养细胞中表达,尤其是在SCT细胞中,在GDM胎盘中表达显著降低。高糖刺激后,forskolin处理的BeWo细胞中ABHD5的表达呈时间依赖性下调。同时,SCT中脂滴(LDs)增加。在siABHD5表达的SCT细胞中,LD储存也增加,而在ABHD5高表达的SCT细胞中,LD储存显著减少。然而,这种作用可以通过下调肉毒碱棕榈酰转移酶1B (CPT1B)来减弱。结论sabhd5 - cpt1b是胎盘脂质代谢的重要调节因子。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
ABHD5-CPT1B: An Important Way of Regulating Placental Lipid Metabolism in Gestational Diabetes Mellitus

Background and Aim

Gestational diabetes mellitus (GDM) is one of the most common metabolic disorders in pregnancy, and a novel association of maternal lipid profile has been suggested to play an important role. However, the molecular mechanism is not clear.

Methods

Bio-analyzed combined with placental metabonomics and single-cell RNA-sequencing (scRNA-seq) successfully identified a potentially important molecule: α-β hydrolase domain-containing protein 5 (ABHD5). The syncytiotrophoblast (SCT) cell model was adopted as a fusion of BeWo cells in response to forskolin. On this basis, the high glucose-stimulated cell experiment was carried out. 15 women with GDM and 15 normal pregnant women were recruited for validation experiments.

Results

ABHD5 was mainly expressed in the trophoblast cells, especially in SCT cells, and significantly decreased in the GDM placenta. After stimulation by high glucose, the expression of ABHD5 was downregulated in a time-dependent manner in BeWo cells treated with forskolin. At the same time, lipid droplets (LDs) were increased in the SCT. LD storage was also increased in the SCT with siABHD5, while it was significantly reduced in SCT cells with high ABHD5 expression. However, this effect could be attenuated by downregulated carnitine palmitoyltransferase 1B (CPT1B).

Conclusions

ABHD5-CPT1B is confirmed as an important regulator of placental lipid metabolism.

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来源期刊
Archives of Medical Research
Archives of Medical Research 医学-医学:研究与实验
CiteScore
12.50
自引率
0.00%
发文量
84
审稿时长
28 days
期刊介绍: Archives of Medical Research serves as a platform for publishing original peer-reviewed medical research, aiming to bridge gaps created by medical specialization. The journal covers three main categories - biomedical, clinical, and epidemiological contributions, along with review articles and preliminary communications. With an international scope, it presents the study of diseases from diverse perspectives, offering the medical community original investigations ranging from molecular biology to clinical epidemiology in a single publication.
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