紫外线和光合有效辐射对蓝细菌下螺旋藻HKAR-19光合效率和抗氧化系统的影响

IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Folia microbiologica Pub Date : 2024-08-01 Epub Date: 2023-12-02 DOI:10.1007/s12223-023-01110-7
Jyoti Jaiswal, Neha Kumari, Amit Gupta, Ashish P Singh, Rajeshwar P Sinha
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引用次数: 0

摘要

本研究综述了藻蓝藻HKAR-19在光合有效辐射(PAR)、PAR+UV-A (PA)和PAR+UV-A+UV-B (PAB)模拟光照条件下的响应。暴露于紫外线辐射导致显著的(P
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Impacts of ultraviolet and photosynthetically active radiations on photosynthetic efficiency and antioxidant systems of the cyanobacterium Spirulina subsalsa HKAR-19.

Impacts of ultraviolet and photosynthetically active radiations on photosynthetic efficiency and antioxidant systems of the cyanobacterium Spirulina subsalsa HKAR-19.

This study summarizes the response of cyanobacterium Spirulina subsalsa HKAR-19 under simulated light conditions of photosynthetically active radiation (PAR), PAR+UV-A (PA), and PAR+UV-A+UV-B (PAB). Exposure to UV radiation caused a significant (P < 0.05) decrease in chlorophyll a, phycocyanin, and total protein. In contrast, total carotene content increased significantly (P < 0.05) under PA and PAB with increasing irradiation time. The photosynthetic efficiency of photosystem II also decreased significantly in PA and PAB radiation. We have also recorded a decrease in the fluorescence emission intensity of phycocyanin under PA and PAB exposure. The phycocyanin fluorescence shifted towards shorter wavelengths (blue-shift) after 72 h of PA and PAB exposure. Intracellular reactive oxygen species (ROS) levels increased significantly in PA and PAB. Fluorescence microscopic images showed an increase in green fluorescence, indicating ROS generation in UV radiation. We have also quantified ROS generation using green and red fluorescence ratio represented as G/R ratio. A 2-6-fold increase in antioxidative enzymes activity was observed to overcome the damaging effects caused by UV stress as compared to untreated control cultures. The lipid peroxidation was assessed in terms of malondialdehyde content which increases significantly (P < 0.05) as the duration of exposure increases. These results suggest that a combined effect of PAR, UV-A, and UV-B was more deleterious than an individual one.

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来源期刊
Folia microbiologica
Folia microbiologica 工程技术-生物工程与应用微生物
CiteScore
5.80
自引率
0.00%
发文量
82
审稿时长
6-12 weeks
期刊介绍: Unlike journals which specialize ever more narrowly, Folia Microbiologica (FM) takes an open approach that spans general, soil, medical and industrial microbiology, plus some branches of immunology. This English-language journal publishes original papers, reviews and mini-reviews, short communications and book reviews. The coverage includes cutting-edge methods and promising new topics, as well as studies using established methods that exhibit promise in practical applications such as medicine, animal husbandry and more. The coverage of FM is expanding beyond Central and Eastern Europe, with a growing proportion of its contents contributed by international authors.
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