脂肪源性干细胞在手术诱导的大鼠白膜损伤愈合中的作用。

IF 2.6 3区 医学 Q1 MEDICINE, GENERAL & INTERNAL
Sexual Medicine Pub Date : 2023-11-20 eCollection Date: 2023-10-01 DOI:10.1093/sexmed/qfad058
Abdallah Sharqawi, Mona F Mansour, Gamal A Elatrash, Ezzat A Ismail, David Ralph, Ahmed I El-Sakka
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引用次数: 0

摘要

背景:将脂肪源性干细胞(ADSCs)注射到受损的白膜(TA)中可以预防纤维化,恢复促纤维化和抗纤维化途径之间的平衡,并可能减轻由TA异常愈合引起的勃起功能障碍。目的:探讨ADSC注射对手术致大鼠TA损伤的结构、超微结构、功能和分子变化的潜在作用。方法:40只成年雄性白化Wistar大鼠随机分为5组,每组8只:第一组,假手术;2组,TA损伤未处理;3组TA损伤及缝合修复;4组,TA损伤后注射ADSCs,不缝合修复;5组,TA损伤后注射ADSCs并缝合修复。结果:6周后,各组均接受功能、组织学、超微结构检查及愈合生长因子的分子表达。结果:海绵内压(ICP);1 ~ 5组平均±SD分别为114±2、32±2、65±2、68±2、111±2 mm Hg。3 ~ 5组与2组ICP比较,差异均有统计学意义(P P P > 0.05)。2组大鼠囊膜组织有明显的组织学和超微结构改变;然而,在第5组,这些变化明显更少,纤维化改变水平更低,弹性变,整体神经内皮细胞表达水平更高。与2组相比,3组和4组的结构和超微结构参数均有所改善。第5组转化生长因子β1和碱性成纤维细胞生长因子表达水平较低。临床意义:该实验模型可能鼓励给药ADSCs以防止创伤对TA的有害影响。优势与局限性:注射ADSC可以改善TA损伤大鼠模型的愈合过程和勃起功能障碍,并且将ADSC注射与手术缝合相结合可以获得更好的结果。主要的限制是缺乏长期的ICP测量和较长的随访期,这可能提供进一步了解愈合过程的慢性阶段。结论:ADSC注射可预防大鼠TA外伤后的结构、超微结构、功能和分子改变,增强创伤后腰膜缝合的效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Role of adipose-derived stem cells in healing surgically induced trauma of the rat's tunica albuginea.

Background: Injection of adipose-derived stem cells (ADSCs) into the injured tunica albuginea (TA) may prevent fibrosis, restore the balance between pro- and antifibrotic pathways, and potentially mitigate erectile dysfunction caused by abnormal TA healing.

Aim: To assess the potential role of ADSC injection on structural, ultrastructural, functional, and molecular changes in surgically induced trauma of the rat's TA.

Methods: Forty adult male albino Wistar rats were divided into 5 groups of 8 rats each: group 1, sham; group 2, injury to TA without treatment; group 3, injury to TA and suture repair; group 4, injury to TA and injection of ADSCs without suture repair; group 5, injury to TA followed by injection of ADSCs and suture repair.

Outcomes: After 6 weeks, all groups were subjected to functional, histologic, and ultrastructural examination and molecular expression of healing growth factors.

Results: The intracavernous pressure (ICP; mean ± SD) was 114 ± 2, 32 ± 2, 65 ± 2, 68 ± 2, and 111 ± 2 mm Hg in groups 1 to 5, respectively. There were significant differences in ICP between each of groups 3 to 5 and group 2 (P < .05), and groups 3 and 4 each had significant differences with group 1 (P < .05). No significant difference in ICP occurred between groups 3 and 4 (P > .05). There were significant histologic and ultrastructural alterations in tunical tissues from group 2; however, these changes were markedly less in group 5 in terms of lower levels of fibrotic changes, elastosis, and superior overall neuroendothelial expression. Groups 3 and 4 showed improved structural and ultrastructural parameters when compared with group 2. Group 5 demonstrated lower levels of transforming growth factor β1 and basic fibroblast growth factor expression.

Clinical implications: This experimental model may encourage administration of ADSCs to prevent the deleterious effects of trauma to the TA.

Strengths and limitations: Injecting ADSCs can improve the healing process and erectile dysfunction in a rat model following TA injury, and combining ADSC injection with surgical suturing resulted in superior outcomes. The main limitation was the absence of long-term ICP measurements and a longer follow-up period that may provide further insight into the chronic phase of the healing process.

Conclusion: ADSC injection may prevent structural, ultrastructural, functional, and molecular alterations in surgically induced trauma of the rat's TA and enhance the effect of tunical suturing after trauma.

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来源期刊
Sexual Medicine
Sexual Medicine MEDICINE, GENERAL & INTERNAL-
CiteScore
5.40
自引率
0.00%
发文量
103
审稿时长
22 weeks
期刊介绍: Sexual Medicine is an official publication of the International Society for Sexual Medicine, and serves the field as the peer-reviewed, open access journal for rapid dissemination of multidisciplinary clinical and basic research in all areas of global sexual medicine, and particularly acts as a venue for topics of regional or sub-specialty interest. The journal is focused on issues in clinical medicine and epidemiology but also publishes basic science papers with particular relevance to specific populations. Sexual Medicine offers clinicians and researchers a rapid route to publication and the opportunity to publish in a broadly distributed and highly visible global forum. The journal publishes high quality articles from all over the world and actively seeks submissions from countries with expanding sexual medicine communities. Sexual Medicine relies on the same expert panel of editors and reviewers as The Journal of Sexual Medicine and Sexual Medicine Reviews.
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