鸡胚移植后性腺生殖细胞的迁移和增殖。

IF 1.8 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Journal of Poultry Science Pub Date : 2023-11-29 eCollection Date: 2023-01-01 DOI:10.2141/jpsa.2023028
Yuki Nakajima, Takahiro Tagami, Atsushi Tajima
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引用次数: 0

摘要

种系嵌合体是体内生殖细胞发育和分化的有效模型。从鸡胚性腺中收集的性腺生殖细胞(GGCs)可作为供体细胞用于生殖系嵌合体的产生。然而,移植到受体胚胎后GGCs的迁移和增殖能力尚不清楚。本研究从7日龄的白莱格霍恩胚胎中收集GGCs并进行荧光标记,将其移植到2.5日龄的罗德岛红(RIR)胚胎的背主动脉中,分析GGCs的迁移和增殖能力。移植5 d后,当每个胚胎移植50个GGCs时,雄性和雌性GGCs在RIR性腺中的数量显著高于非性腺的数量。为了分析GGCs在中胚层的时间迁移,我们再次转移了50个GGCs。移植后第3 ~ 6天,RIR生殖腺中雄性和雌性GGCs数量显著增加。为了分析GGC在性腺中的迁移和增殖,我们将单个GGC分别移植到100个雄性和100个雌性胚胎中。移植后5 d,雄性GGCs沉降率为37%(37/100),增殖率为24%(24/100),雌性GGCs沉降率为23%(23/100),增殖率为8%(8/100)。因此,GGCs是一种异质细胞群,可能具有也可能不具有迁移和增殖能力。GGCs在女性中的异质性可能大于男性。当移植50个GGCs时,胚胎中存在的几乎所有GGCs都在性腺和中肾中定居和增殖。GGCs在受体性腺内的迁移和增殖能力在个体间或供体性别间存在很大差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Gonadal Germ Cell Migration and Proliferation after Transfer in Developing Chicken Embryos.

A germline chimera is a useful model for developing and differentiating germ cells in vivo. Gonadal germ cells (GGCs) collected from chicken embryonic gonads may be used to produce germline chimeras as donor cells. However, the migratory and proliferative abilities of GGCs after transfer into recipient embryos are unclear. Here, the migratory and proliferative abilities of GGCs collected from 7-day-old White Leghorn embryos and fluorescently labeled were analyzed following transfer into the dorsal aorta of 2.5-day-old Rhode Island Red (RIR) embryos. Five days after transfer, the numbers of male and female GGCs were significantly higher in the RIR gonads than those in non-gonadal RIR organs when 50 GGCs were transferred per embryo. To analyze the temporal migration of GGCs in intermediate mesoderm, 50 GGCs were again transferred. The numbers of male and female GGCs in RIR gonads increased significantly from days 3 to 6 after transfer. To analyze GGC migration and proliferation in the gonads, a single GGC was transferred into 100 male and 100 female embryos. Five days after transfer, the frequencies of settled and proliferated GGCs were 37% (37/100) and 24% (24/100) in males, and 23% (23/100) and 8% (8/100) in females, respectively. Thus, GGCs are a heterogeneous cell population that may or may not have migratory and proliferative abilities. The heterogeneity of GGCs may be greater in females than that in males. When 50 GGCs were transplanted, almost all those present in embryos had settled and proliferated in the gonads and mesonephros. The migratory and proliferative abilities of GGCs in recipient gonads were considerably diverse in individual GGCs or between donor sexes.

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来源期刊
Journal of Poultry Science
Journal of Poultry Science AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
2.80
自引率
13.30%
发文量
26
审稿时长
12 months
期刊介绍: The Journal of Poultry Science will publish original reports and reviews which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: breeding and genetics, nutrition and feeds, physiology, reproduction, immunology, behavior, environmental science, management and housing welfare, processing and products, and health in poultry. Submission of original articles to the Journal is open to all poultry researchers. The review articles are invited papers written by international outstanding researchers. Articles will be published in English, American style.
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