{"title":"经颅直流电刺激通过降低脊髓NMDAR2B表达减轻骨关节炎大鼠慢性疼痛。","authors":"Zhihua Liu, Xia Chen, Peng Chen, Lili Wang","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>Osteoarthritis (OA) has been the common cause to lead to chronic pain. Transcranial direct current stimulation (tDCS) is effective in the treatment of chronic pain, but its analgesic mechanism is still unclear. This study observed the analgesic effects of tDCS in rats to explore the top-down analgesic modulation mechanism of tDCS.</p><p><strong>Methods: </strong>Monosodium iodoacetate (MIA) was used to establish OA chronic pain model. After 21 days, the rats received tDCS for 14 consecutive days (20 min/day). We assessed the pain-related behaviors of rats at different time points. Western blot and Immunohistochemistry were performed to observe the expression level of NMDAR2B in the spinal cord after tDCS treatment.</p><p><strong>Results: </strong>After MIA injection, rats developed apparent mechanical hyperalgesia and thermal hyperalgesia. However, the pain-related behaviors of rats were significantly improved after tDCS treatment. In addition, the expression of NMDAR2B and the proportion of positive stained cells of NMDAR2B were reversed by tDCS treatment.</p><p><strong>Conclusions: </strong>The results demonstrated that tDCS can attenuate OA-induced chronic pain in rats via reducing NMDAR2B expressions in the spinal cord. We believe that this may be the result of tDCS participating in the top-down modulation of pain pathway in the endogenous analgesic system.</p>","PeriodicalId":16430,"journal":{"name":"Journal of musculoskeletal & neuronal interactions","volume":"23 4","pages":"498-505"},"PeriodicalIF":1.7000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10696371/pdf/","citationCount":"0","resultStr":"{\"title\":\"Transcranial Direct Current Stimulation Attenuates the Chronic Pain of Osteoarthritis in Rats via Reducing NMDAR2B Expressions in the Spinal Cord.\",\"authors\":\"Zhihua Liu, Xia Chen, Peng Chen, Lili Wang\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>Osteoarthritis (OA) has been the common cause to lead to chronic pain. Transcranial direct current stimulation (tDCS) is effective in the treatment of chronic pain, but its analgesic mechanism is still unclear. This study observed the analgesic effects of tDCS in rats to explore the top-down analgesic modulation mechanism of tDCS.</p><p><strong>Methods: </strong>Monosodium iodoacetate (MIA) was used to establish OA chronic pain model. After 21 days, the rats received tDCS for 14 consecutive days (20 min/day). We assessed the pain-related behaviors of rats at different time points. Western blot and Immunohistochemistry were performed to observe the expression level of NMDAR2B in the spinal cord after tDCS treatment.</p><p><strong>Results: </strong>After MIA injection, rats developed apparent mechanical hyperalgesia and thermal hyperalgesia. However, the pain-related behaviors of rats were significantly improved after tDCS treatment. In addition, the expression of NMDAR2B and the proportion of positive stained cells of NMDAR2B were reversed by tDCS treatment.</p><p><strong>Conclusions: </strong>The results demonstrated that tDCS can attenuate OA-induced chronic pain in rats via reducing NMDAR2B expressions in the spinal cord. We believe that this may be the result of tDCS participating in the top-down modulation of pain pathway in the endogenous analgesic system.</p>\",\"PeriodicalId\":16430,\"journal\":{\"name\":\"Journal of musculoskeletal & neuronal interactions\",\"volume\":\"23 4\",\"pages\":\"498-505\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2023-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10696371/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of musculoskeletal & neuronal interactions\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"NEUROSCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of musculoskeletal & neuronal interactions","FirstCategoryId":"3","ListUrlMain":"","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"NEUROSCIENCES","Score":null,"Total":0}
Transcranial Direct Current Stimulation Attenuates the Chronic Pain of Osteoarthritis in Rats via Reducing NMDAR2B Expressions in the Spinal Cord.
Objectives: Osteoarthritis (OA) has been the common cause to lead to chronic pain. Transcranial direct current stimulation (tDCS) is effective in the treatment of chronic pain, but its analgesic mechanism is still unclear. This study observed the analgesic effects of tDCS in rats to explore the top-down analgesic modulation mechanism of tDCS.
Methods: Monosodium iodoacetate (MIA) was used to establish OA chronic pain model. After 21 days, the rats received tDCS for 14 consecutive days (20 min/day). We assessed the pain-related behaviors of rats at different time points. Western blot and Immunohistochemistry were performed to observe the expression level of NMDAR2B in the spinal cord after tDCS treatment.
Results: After MIA injection, rats developed apparent mechanical hyperalgesia and thermal hyperalgesia. However, the pain-related behaviors of rats were significantly improved after tDCS treatment. In addition, the expression of NMDAR2B and the proportion of positive stained cells of NMDAR2B were reversed by tDCS treatment.
Conclusions: The results demonstrated that tDCS can attenuate OA-induced chronic pain in rats via reducing NMDAR2B expressions in the spinal cord. We believe that this may be the result of tDCS participating in the top-down modulation of pain pathway in the endogenous analgesic system.
期刊介绍：
The Journal of Musculoskeletal and Neuronal Interactions (JMNI) is an academic journal dealing with the pathophysiology and treatment of musculoskeletal disorders. It is published quarterly (months of issue March, June, September, December). Its purpose is to publish original, peer-reviewed papers of research and clinical experience in all areas of the musculoskeletal system and its interactions with the nervous system, especially metabolic bone diseases, with particular emphasis on osteoporosis. Additionally, JMNI publishes the Abstracts from the biannual meetings of the International Society of Musculoskeletal and Neuronal Interactions, and hosts Abstracts of other meetings on topics related to the aims and scope of JMNI.
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