毛茛叶水提物对百草枯诱导的帕金森病样成年Wistar大鼠氧化应激和多巴胺能神经元失能的保护作用。

IF 2.7 4区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Felix U. Enemali, Kingsley Afoke Iteire, Raphael E. Uweigho, Ogunberi Blessing, Gbayisomore Tolulope Judah
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引用次数: 0

摘要

研究背景:毛茛具有很高的营养价值,对多种疾病的管理和治疗有益。本研究评价了毛茛叶水提物对百草枯(PQ)致Wistar大鼠黑质神经毒性的作用。材料和方法:采用开放式实验(OFT)评估毛茛叶水提取物的运动活性,氧化应激生物标志物[过氧化氢酶(CAT)和超氧化物歧化酶(SOD)],组织学检查(H和E染色)检测细胞结构变化,免疫组织化学研究使用酪氨酸羟化酶(TH)作为多巴胺能神经元的标记。42只大鼠分为6组(n = 7);组1:对照组给予0.5ml/kg蒸馏水,组2:给予10mg/kg PQ + 10mg/kg左旋多巴作为对照药,组3;第4组:分别给予10mg/kg PQ + 200mg/kg阿玛兰,第5组:给予10mg/kg PQ + 300mg/kg阿玛兰,第6组:给予10mg/kg PQ + 400mg/kg阿玛兰,连续14天。所有给药均为口服;结果和讨论中有一个显著的差异:该研究的野外测试(OFT)显示百草枯(PQ)暴露后没有运动活动缺陷。此外,与对照组和其他经左旋多巴和紫花草预处理的组相比,仅使用百草枯(PQ)治疗组未观察到细胞结构扭曲。此外,与对照组和左旋多巴预处理组相比,百草枯(PQ)处理组表现出氧化应激,抗氧化酶(SOD)显著降低。在百草枯(PQ)单独处理下,酪氨酸羟化酶(TH)表达多巴胺能神经元显著减少。然而,相对于对照组,野田螺处理通过显著增加酪氨酸羟化酶(TH)表达多巴胺能神经元水平显示出治疗特性。结论:毛茛叶水提物对百草枯(PQ)致Wistar大鼠黑质改变具有治疗作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Aqueous leaf extract of Phyllanthus amarus protects against oxidative stress and misfiring of dopaminergic neurons in Paraquat-induced Parkinson’s disease-like model of adult Wistar rats

Background of the study

Phyllanthus amarus has high nutritional value and is beneficial in managing and treating diverse ailments. This study assessed the role of aqueous leaf extract of Phyllanthus amarus on Paraquat (PQ) induced neurotoxicity in the substantia nigra of Wistar rats.

Materials and methods

The role of aqueous leaves extract of Phyllanthus amarus was assessed using an open field test (OFT) for motor activity, oxidative stress biomarkers [Catalase (CAT), and Superoxide Dismutase (SOD)], histological examination (H and E stained) for cytoarchitectural changes and immunohistochemical studies using tyrosine hydroxylase (TH) as a marker for dopaminergic neurons. Forty-two (42) rats were categorized into six groups (n = 7); group 1: control was administered 0.5 ml/kg distilled water, group 2: received 10 mg/kg PQ + 10 mg/kg L-dopa as reference drug, group 3; received 10 mg/kg PQ, while group 4: received 10 mg/kg PQ + 200 mg/kg P. amarus, group 5: received 10 mg/kg PQ + 300 mg/kg P. amarus, and group 6: received 10 mg/kg PQ + 400 mg/kg P. amarus respectively, for 14 days. All administrations were done orally; a significant difference was set at p < 0.05.

Results and discussion

The study's open field test (OFT) revealed no motor activity deficit with Paraquat (PQ) exposure. Also, cytoarchitectural distortions were not observed with Paraquat (PQ) only treatment group compared to the control and other groups pretreated with P. amarus and L-dopa. Moreover, the Paraquat (PQ) only treatment group showed oxidative stress by significantly decreasing the antioxidant enzyme (SOD) compared to the control and L-dopa pretreated group. A significant decrease in tyrosine hydroxylase (TH) expressing dopaminergic neurons was also observed in Paraquat (PQ) only treatment. However, P. amarus treatment showed therapeutic properties by significantly increasing tyrosine hydroxylase (TH) expressing dopaminergic neuron levels relative to control.

Conclusion

Aqueous leaf extract of Phyllanthus amarus possesses therapeutic properties against Paraquat (PQ) induced changes in the substantia nigra of Wistar rats.

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来源期刊
Journal of chemical neuroanatomy
Journal of chemical neuroanatomy 医学-神经科学
CiteScore
4.50
自引率
3.60%
发文量
87
审稿时长
62 days
期刊介绍: The Journal of Chemical Neuroanatomy publishes scientific reports relating the functional and biochemical aspects of the nervous system with its microanatomical organization. The scope of the journal concentrates on reports which combine microanatomical, biochemical, pharmacological and behavioural approaches. Papers should offer original data correlating the morphology of the nervous system (the brain and spinal cord in particular) with its biochemistry. The Journal of Chemical Neuroanatomy is particularly interested in publishing important studies performed with up-to-date methodology utilizing sensitive chemical microassays, hybridoma technology, immunocytochemistry, in situ hybridization and receptor radioautography, to name a few examples. The Journal of Chemical Neuroanatomy is the natural vehicle for integrated studies utilizing these approaches. The articles will be selected by the editorial board and invited reviewers on the basis of their excellence and potential contribution to this field of neurosciences. Both in vivo and in vitro integrated studies in chemical neuroanatomy are appropriate subjects of interest to the journal. These studies should relate only to vertebrate species with particular emphasis on the mammalian and primate nervous systems.
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