慢性鼻窦炎鼻息肉和鼻液蛋白和基因表达特征的比较。

IF 2.5 4区 医学 Q3 ALLERGY
International Archives of Allergy and Immunology Pub Date : 2024-01-01 Epub Date: 2023-11-29 DOI:10.1159/000534226
Yilin Hou, Changhui Chen, Zhengqi Li, Tong Lu, Lin Sun, Yi Wei, Jian Li, Weiping Wen
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引用次数: 0

摘要

慢性鼻窦炎伴鼻息肉(CRSwNP)是一种严重的炎症性疾病。鼻液(NFs)为研究CRSwNP发病机制提供了一种非侵入性的鼻活检替代方法。我们的目的是比较鼻息肉(NPs)和NFs之间的蛋白质和mRNA炎症特征。方法:采用Luminex法比较聚乙烯醇(PVA)海绵和NFs可吸收装置(NFAD)采集20例CRSwNP患者NFs的性能。另一组包括4名健康对照和另外21名CRSwNP患者(包括嗜酸性CRSwNP [ECRSwNP]和非嗜酸性CRSwNP [NECRSwNP]),通过Olink平台进行蛋白定量和rna测序进行基因表达评估。采用Spearman分析检测NFs中蛋白表达水平与临床评估变量之间的相关性。结果:nfad收集的NFs中细胞因子浓度比PVA海绵中细胞因子浓度至少高2倍,且细胞因子水平与NPs显著相关(ρ >0.45, p <0.05)。与对照组相比,在ECRSwNP亚组中,NFs和NPs之间的差异表达蛋白显著相关(ρ = 0.41, p <0.01)。在ECRSwNP患者中,Th2/IL-13、MCP4和CCL4水平升高,这是嗜酸性粒细胞浸润的特征。基因与蛋白表达呈显著相关(ρ = 0.34, p <0.01)。NFs患者的PDL2水平与ECRSwNP术后复发、鼻腔VAS和SNOT-22评分呈正相关(ρ >0.68, p <0.05)。结论:我们的研究揭示了同一CRSwNP患者中NPs和NFs之间炎症特征的相似性和差异性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparing Protein and Gene Expression Signature between Nasal Polyps and Nasal Fluids in Chronic Rhinosinusitis.

Introduction: Chronic rhinosinusitis with nasal polyps (CRSwNP) is a serious inflammatory condition. Nasal fluids (NFs) present a noninvasive alternative to nasal biopsy for studying CRSwNP pathogenesis. We aimed to compare the protein and mRNA inflammation signature between nasal polyps (NPs) and NFs.

Method: The performance of polyvinyl alcohol (PVA) sponges and NFs absorbable device (NFAD) for collecting NFs from 20 patients with CRSwNP was compared using the Luminex assay. The other group consisted of four healthy controls and an additional 21 CRSwNP patients (including eosinophilic CRSwNP [ECRSwNP] and non-eosinophilic CRSwNP [NECRSwNP]) for protein quantification by Olink platform and gene expression evaluation by RNA-sequencing. Spearman's analysis was performed to detect correlations between protein expression levels in NFs and clinical assessment variables.

Results: NFAD-collected NFs contained at least a 2-fold higher concentration of cytokines than that obtained using PVA sponge, and these cytokines levels are significantly associated with NPs (ρ > 0.45, p < 0.05). Differentially expressed proteins between NFs and NPs were significantly correlated in the ECRSwNP subgroup compared with controls (ρ = 0.41, p < 0.01). Levels of Th2/IL-13, MCP4, and CCL4, characteristic of eosinophilic infiltration, were increased in ECRSwNP patients. A significant correlation between gene and protein expression was observed (ρ = 0.34, p < 0.01). PDL2 levels in NFs were positively correlated with ECRSwNP postoperative recurrence, the nasal VAS, and SNOT-22 scores (ρ > 0.68, p < 0.05 for all).

Conclusion: Our study revealed similarities and discrepancies in inflammatory signatures between NPs and NFs in the same CRSwNP patient.

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来源期刊
CiteScore
5.60
自引率
3.60%
发文量
105
审稿时长
2 months
期刊介绍: ''International Archives of Allergy and Immunology'' provides a forum for basic and clinical research in modern molecular and cellular allergology and immunology. Appearing monthly, the journal publishes original work in the fields of allergy, immunopathology, immunogenetics, immunopharmacology, immunoendocrinology, tumor immunology, mucosal immunity, transplantation and immunology of infectious and connective tissue diseases.
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