催化o -甲基化黄酮形成的o -甲基转移酶的多样性和区域选择性。

IF 8.1 2区 工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Critical Reviews in Biotechnology Pub Date : 2024-09-01 Epub Date: 2023-11-30 DOI:10.1080/07388551.2023.2280755
Juan Wang, Ning Liao, Guanwen Liu, Yinghui Li, Fengqin Xu, Junling Shi
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引用次数: 0

摘要

黄酮类化合物及其甲基化衍生物具有抗菌、抗炎、抗癌等多种功效,在食品和生物医药领域具有巨大的市场潜力。类黄酮及其衍生物的生物合成通常是通过使用转基因微生物来确保大规模生产。因此,了解介导类黄酮甲基化的o -甲基转移酶(OMTs)的特性是至关重要的。然而,这些omt的性质取决于它们的来源、底物特异性、活性位点的氨基酸残基以及复杂的机制。为了获得选择合适的omt用于目标甲基化类黄酮生物合成的线索,我们对目前报道的结果进行了全面的回顾,特别关注它们对不同类黄酮底物的比较区域选择性。此外,利用分子模拟技术探讨了该类酶多样性的可能机制。最后,讨论了我们在认识上的主要差距和未来研究的领域。该研究结果可能有助于选择编码omt的基因和设计基于酶的合成o -甲基化黄酮类化合物的过程。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Diversity and regioselectivity of O-methyltransferases catalyzing the formation of O-methylated flavonoids.

Flavonoids and their methylated derivatives have immense market potential in the food and biomedical industries due to their multiple beneficial effects, such as antimicrobial, anti-inflammatory, and anticancer activities. The biological synthesis of flavonoids and their derivatives is often accomplished via the use of genetically modified microorganisms to ensure large-scale production. Therefore, it is pivotal to understand the properties of O-methyltransferases (OMTs) that mediate the methylation of flavonoids. However, the properties of these OMTs are governed by their: sources, substrate specificity, amino acid residues in the active sites, and the intricate mechanism. In order to obtain a clue for the selection of suitable OMTs for the biosynthesis of a target methylated flavonoid, we made a comprehensive review of the currently reported results, with a particular focus on their comparative regioselectivity for different flavonoid substrates. Additionally, the possible mechanisms for the diversity of this class of enzymes were explored using molecular simulation technology. Finally, major gaps in our understanding and areas for future studies were discussed. The findings of this study may be useful in selecting genes that encode OMTs and designing enzyme-based processes for synthesizing O-methylated flavonoids.

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来源期刊
Critical Reviews in Biotechnology
Critical Reviews in Biotechnology 工程技术-生物工程与应用微生物
CiteScore
20.80
自引率
1.10%
发文量
71
审稿时长
4.8 months
期刊介绍: Biotechnological techniques, from fermentation to genetic manipulation, have become increasingly relevant to the food and beverage, fuel production, chemical and pharmaceutical, and waste management industries. Consequently, academic as well as industrial institutions need to keep abreast of the concepts, data, and methodologies evolved by continuing research. This journal provides a forum of critical evaluation of recent and current publications and, periodically, for state-of-the-art reports from various geographic areas around the world. Contributing authors are recognized experts in their fields, and each article is reviewed by an objective expert to ensure accuracy and objectivity of the presentation.
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