双酚A通过促进NLRP3的o - glcn酰化诱导非酒精性脂肪肝。

IF 2.5 4区 医学 Q3 ENDOCRINOLOGY & METABOLISM
Yonghong Zhang, Shujuan Han, Tian Li, Li Zhu, Feng Wei
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引用次数: 0

摘要

非酒精性脂肪性肝病(NAFLD)是最常见的肝病。双酚A (BPA)促进NAFLD的机制尚不清楚。采用棕榈酸(PA)和脂多糖(LPS)体外模拟HepG2细胞NAFLD。测定总胆固醇(TC)、甘油三酯(TG)含量和脂质积累来评估脂质代谢。观察caspase-1染色的细胞和NLRP3炎性小体相关蛋白是否存在焦亡。Western blot法检测蛋白水平,Co-IP法检测蛋白之间的相关性。环己亚胺(CHX)联合western blot检测蛋白稳定性。这些数据表明BPA通过上调O-GlcNAc转移酶(OGT)水平诱导脂质代谢功能障碍和焦亡。NLRP3直接与OGT相互作用,升高的OGT增强了NLRP3蛋白的稳定性。BPA促进ogt介导的o - glcn酰化以稳定NLRP3,从而加速体外NAFLD的进展。我们的研究表明BPA作为一种环境因素可能参与了NAFLD的促进,靶向NLRP3和OGT可能抑制BPA对NAFLD的诱导。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Bisphenol A induces non-alcoholic fatty liver disease by promoting the O-GlcNAcylation of NLRP3.

Non-alcoholic fatty liver disease (NAFLD) is the most common liver disease. The mechanism by which bisphenol A (BPA) promots NAFLD remains unclear. Palmitic acid (PA) and lipopolysaccharide (LPS) were used to simulate NAFLD in HepG2 cells in vitro. Total cholesterol (TC), triglyceride (TG) content, and lipid accumulation were measured to evaluate lipid metabolism. The caspase-1-stained cells and NLRP3 inflammasome-associated proteins were evaluated for pyroptosis. Western blot analysis was used to detect protein levels and co-immunoprecipitation (Co-IP) was used to detect the association between the proteins. Cycloheximide (CHX) treatment combined with western blot was performed to access protein stability. This data have shown that BPA induces lipid metabolism dysfunction and pyroptosis by upregulating O-GlcNAc transferase (OGT) level. NLRP3 directly interacts with OGT, and elevated OGT enhanced the stability of NLRP3 protein. BPA promoted OGT-mediated O-GlcNAcylation to stabilised NLRP3, thus accelerating NAFLD progress in vitro. Our study reveals that BPA, as an environmental factor, may be involved in the promotion of NAFLD, and that targeting NLRP3 and OGT may inhibit BPA's induction of NAFLD.

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来源期刊
Archives of Physiology and Biochemistry
Archives of Physiology and Biochemistry ENDOCRINOLOGY & METABOLISM-PHYSIOLOGY
CiteScore
6.90
自引率
3.30%
发文量
21
期刊介绍: Archives of Physiology and Biochemistry: The Journal of Metabolic Diseases is an international peer-reviewed journal which has been relaunched to meet the increasing demand for integrated publication on molecular, biochemical and cellular aspects of metabolic diseases, as well as clinical and therapeutic strategies for their treatment. It publishes full-length original articles, rapid papers, reviews and mini-reviews on selected topics. It is the overall goal of the journal to disseminate novel approaches to an improved understanding of major metabolic disorders. The scope encompasses all topics related to the molecular and cellular pathophysiology of metabolic diseases like obesity, type 2 diabetes and the metabolic syndrome, and their associated complications. Clinical studies are considered as an integral part of the Journal and should be related to one of the following topics: -Dysregulation of hormone receptors and signal transduction -Contribution of gene variants and gene regulatory processes -Impairment of intermediary metabolism at the cellular level -Secretion and metabolism of peptides and other factors that mediate cellular crosstalk -Therapeutic strategies for managing metabolic diseases Special issues dedicated to topics in the field will be published regularly.
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