Povilas Sakalauskas , Evelina Kaminskienė , Dovilė Bukauskaitė , Vytautas Eigirdas , Justina Snegiriovaitė , Dalytė Mardosaitė-Busaitienė , Algimantas Paulauskas
{"title":"立陶宛蝙蝠巴贝斯虫的分子检测","authors":"Povilas Sakalauskas , Evelina Kaminskienė , Dovilė Bukauskaitė , Vytautas Eigirdas , Justina Snegiriovaitė , Dalytė Mardosaitė-Busaitienė , Algimantas Paulauskas","doi":"10.1016/j.ttbdis.2023.102283","DOIUrl":null,"url":null,"abstract":"<div><p><em>Babesia vesperuginis</em> is an intraerythrocytic protozoan parasite that circulates among bats and ticks in many countries worldwide. However, the distribution of <em>B. vesperuginis</em> in the Baltic region has not been studied. A total of 86 dead bats from eight different species were collected and screened for <em>Babesia</em> spp. using real-time PCR. Overall, 52.3% (45/86) of the bats were found positive for <em>Babesia</em> spp. The prevalence of <em>Babesia</em> spp. in different organs varied, with the highest prevalence observed in heart tissues (37.0%) and the lowest in liver tissues (22.2%). However, the observed differences in prevalence among organs were not statistically significant. Blood samples from 125 bats of nine different species were also analyzed for <em>Babesia</em> spp. prevalence using real-time PCR and nested PCR. The results showed a prevalence of 35.2% and 22.4%, respectively. Moreover, 28.3% (17/60) of the examined blood samples were confirmed positive for <em>Babesia</em> spp. through blood smear analysis. The total of 32 partial sequences of the 18S rRNA gene derived in this study were 100% identical to <em>B. vesperuginis</em> sequences from GenBank. In eight species of bats, <em>Pipistrellus nathusii, Pipistrellus pipistrellus, Pipistrellus pygmaeus, Vespertilio murinus, Eptesicus nilssonii, Eptesicus serotinus, Myotis daubentonii</em> and <em>Nyctalus noctula, Babesia</em> parasites were identified. In <em>E. nilssonii, Babesia</em> spp. was identified for the first time.</p></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 1","pages":"Article 102283"},"PeriodicalIF":3.1000,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1877959X23001644/pdfft?md5=725ac442a54f4e4593358c87194c2123&pid=1-s2.0-S1877959X23001644-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Molecular detection of Babesia vesperuginis in bats from Lithuania\",\"authors\":\"Povilas Sakalauskas , Evelina Kaminskienė , Dovilė Bukauskaitė , Vytautas Eigirdas , Justina Snegiriovaitė , Dalytė Mardosaitė-Busaitienė , Algimantas Paulauskas\",\"doi\":\"10.1016/j.ttbdis.2023.102283\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><em>Babesia vesperuginis</em> is an intraerythrocytic protozoan parasite that circulates among bats and ticks in many countries worldwide. However, the distribution of <em>B. vesperuginis</em> in the Baltic region has not been studied. A total of 86 dead bats from eight different species were collected and screened for <em>Babesia</em> spp. using real-time PCR. Overall, 52.3% (45/86) of the bats were found positive for <em>Babesia</em> spp. The prevalence of <em>Babesia</em> spp. in different organs varied, with the highest prevalence observed in heart tissues (37.0%) and the lowest in liver tissues (22.2%). However, the observed differences in prevalence among organs were not statistically significant. Blood samples from 125 bats of nine different species were also analyzed for <em>Babesia</em> spp. prevalence using real-time PCR and nested PCR. The results showed a prevalence of 35.2% and 22.4%, respectively. Moreover, 28.3% (17/60) of the examined blood samples were confirmed positive for <em>Babesia</em> spp. through blood smear analysis. The total of 32 partial sequences of the 18S rRNA gene derived in this study were 100% identical to <em>B. vesperuginis</em> sequences from GenBank. In eight species of bats, <em>Pipistrellus nathusii, Pipistrellus pipistrellus, Pipistrellus pygmaeus, Vespertilio murinus, Eptesicus nilssonii, Eptesicus serotinus, Myotis daubentonii</em> and <em>Nyctalus noctula, Babesia</em> parasites were identified. In <em>E. nilssonii, Babesia</em> spp. was identified for the first time.</p></div>\",\"PeriodicalId\":49320,\"journal\":{\"name\":\"Ticks and Tick-borne Diseases\",\"volume\":\"15 1\",\"pages\":\"Article 102283\"},\"PeriodicalIF\":3.1000,\"publicationDate\":\"2023-11-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1877959X23001644/pdfft?md5=725ac442a54f4e4593358c87194c2123&pid=1-s2.0-S1877959X23001644-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Ticks and Tick-borne Diseases\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1877959X23001644\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ticks and Tick-borne Diseases","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1877959X23001644","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Molecular detection of Babesia vesperuginis in bats from Lithuania
Babesia vesperuginis is an intraerythrocytic protozoan parasite that circulates among bats and ticks in many countries worldwide. However, the distribution of B. vesperuginis in the Baltic region has not been studied. A total of 86 dead bats from eight different species were collected and screened for Babesia spp. using real-time PCR. Overall, 52.3% (45/86) of the bats were found positive for Babesia spp. The prevalence of Babesia spp. in different organs varied, with the highest prevalence observed in heart tissues (37.0%) and the lowest in liver tissues (22.2%). However, the observed differences in prevalence among organs were not statistically significant. Blood samples from 125 bats of nine different species were also analyzed for Babesia spp. prevalence using real-time PCR and nested PCR. The results showed a prevalence of 35.2% and 22.4%, respectively. Moreover, 28.3% (17/60) of the examined blood samples were confirmed positive for Babesia spp. through blood smear analysis. The total of 32 partial sequences of the 18S rRNA gene derived in this study were 100% identical to B. vesperuginis sequences from GenBank. In eight species of bats, Pipistrellus nathusii, Pipistrellus pipistrellus, Pipistrellus pygmaeus, Vespertilio murinus, Eptesicus nilssonii, Eptesicus serotinus, Myotis daubentonii and Nyctalus noctula, Babesia parasites were identified. In E. nilssonii, Babesia spp. was identified for the first time.
期刊介绍：
Ticks and Tick-borne Diseases is an international, peer-reviewed scientific journal. It publishes original research papers, short communications, state-of-the-art mini-reviews, letters to the editor, clinical-case studies, announcements of pertinent international meetings, and editorials.
The journal covers a broad spectrum and brings together various disciplines, for example, zoology, microbiology, molecular biology, genetics, mathematical modelling, veterinary and human medicine. Multidisciplinary approaches and the use of conventional and novel methods/methodologies (in the field and in the laboratory) are crucial for deeper understanding of the natural processes and human behaviour/activities that result in human or animal diseases and in economic effects of ticks and tick-borne pathogens. Such understanding is essential for management of tick populations and tick-borne diseases in an effective and environmentally acceptable manner.
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