没食子酸改善应激大鼠前额皮质和海马的行为功能障碍、氧化损伤和神经元丢失。

IF 2.7 4区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Gholam Hossein Meftahi , Nahid Aboutaleb
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引用次数: 0

摘要

没食子酸(GA)是一种天然酚类化合物,具有抗氧化和神经保护作用。本研究旨在探讨GA对雄性Wistar大鼠应激性氧化损伤、焦虑样行为、神经元丧失和空间学习记忆障碍的影响。将大鼠分为4组(n = 8),分别连续14天每天施加约束应激4小时或不受干扰(对照组不诱导应激)。治疗组给予生理盐水2mL + 100mg/kg GA / d,连续14 d (STR + GA组)。小鼠在诱导约束应激前2h灌胃给药或生理盐水。ELISA法测定氧化应激因子。高架迷宫和莫里斯水迷宫测试分别评估了焦虑样行为和空间学习和记忆。采用尼氏染色法测定神经元密度。抑制应激使应激大鼠MDA含量显著升高,GPX和SOD活性显著降低,而100mg/kg GA对应激大鼠的GPX和SOD活性有保留作用。约束应激显著增强了GA保留的焦虑样行为和空间学习记忆障碍。此外,GA治疗减少了应激大鼠海马和前额叶皮质(PFC)区域的神经元损失。综上所述,我们的研究结果表明,GA有可能通过减少氧化损伤来减轻由约束应激引起的神经行为障碍以及海马和PFC中的神经元损失。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Gallic acid ameliorates behavioral dysfunction, oxidative damage, and neuronal loss in the prefrontal cortex and hippocampus in stressed rats

Gallic acid (GA) is known to be a natural phenolic compound with antioxidant and neuroprotective effects. This study aims to investigate the impact of GA against restraint stress-induced oxidative damage, anxiety-like behavior, neuronal loss, and spatial learning and memory impairment in male Wistar rats. The animals were divided into four groups (n = 8) and subjected to restraint stress for 4 h per day for 14 consecutive days or left undisturbed (control without inducing stress). In the treatment group, the animals were treated with 2 mL normal saline plus 100 mg/kg GA per day for 14 consecutive days (STR + GA group). The animals received the drug or normal saline by gavage 2 h before inducing restraint stress. ELISA assay measured oxidative stress factors. Elevated-plus maze and Morris water maze tests assessed anxiety-like behavior and spatial learning and memory, respectively. Also, neuronal density was determined using Nissl staining. Restraint stress significantly increased MDA and reduced the activities of GPX and SOD in the stressed rats, which were reserved by treatment with 100 mg/kg GA. Restraint stress markedly enhanced the anxiety-like behavior and spatial learning and memory impairment that were reserved by GA. In addition, treatment with GA reduced the neuronal loss in the stressed rats in the hippocampus and prefrontal cortex (PFC) regions. Taken together, our findings suggest that GA has the potential to be used as a good candidate to attenuate neurobehavioral disorders as well as neuronal loss in the hippocampus and PFC induced by restraint stress via reducing oxidative damage.

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来源期刊
Journal of chemical neuroanatomy
Journal of chemical neuroanatomy 医学-神经科学
CiteScore
4.50
自引率
3.60%
发文量
87
审稿时长
62 days
期刊介绍: The Journal of Chemical Neuroanatomy publishes scientific reports relating the functional and biochemical aspects of the nervous system with its microanatomical organization. The scope of the journal concentrates on reports which combine microanatomical, biochemical, pharmacological and behavioural approaches. Papers should offer original data correlating the morphology of the nervous system (the brain and spinal cord in particular) with its biochemistry. The Journal of Chemical Neuroanatomy is particularly interested in publishing important studies performed with up-to-date methodology utilizing sensitive chemical microassays, hybridoma technology, immunocytochemistry, in situ hybridization and receptor radioautography, to name a few examples. The Journal of Chemical Neuroanatomy is the natural vehicle for integrated studies utilizing these approaches. The articles will be selected by the editorial board and invited reviewers on the basis of their excellence and potential contribution to this field of neurosciences. Both in vivo and in vitro integrated studies in chemical neuroanatomy are appropriate subjects of interest to the journal. These studies should relate only to vertebrate species with particular emphasis on the mammalian and primate nervous systems.
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