{"title":"与胃肠道感染相关的肠杆菌科病原菌外膜孔蛋白(Omp) L中保守免疫优势表位肽的定位","authors":"Harish Babu Kolla , Shivakiran Satyanarayan Makam , Prakash Narayana Reddy","doi":"10.1186/s43141-023-00622-6","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>Members of <em>Enterobacteriaceae</em> such as <em>Escherichia coli</em> O 157:H7, <em>Salmonella</em> sp., <em>Shigella</em> sp., <em>Klebsiella</em> sp., and <em>Citrobacter freundii</em> are responsible for the outbreak of serious foodborne illness and other mucosal infections across the globe. The outer membrane proteins (OMPs) of <em>Enterobacteriaceae</em> are highly immunogenic in eliciting immune responses against pathogens. Moreover, the OMPs are highly conserved in the <em>Enterobacteriaceae</em> family. Sequence homology in the OMPs will ensure the presence of conserved immunodominant regions with predominant epitopes. The OmpL is such an immunogen that is highly conserved among the <em>Enterobacteriaceae</em> pathogens. In this study, we performed computational analysis on the outer membrane porin (Omp) L of prominent <em>Enterobacteriaceae</em> pathogens.</div></div><div><h3>Results</h3><div>Multiple sequence and structural alignment analysis have revealed that the OmpL protein is highly conserved among the selected <em>Enterobacteriaceae</em> pathogens. This amount of sequence and structural homology uncovered the conserved antibody binding B-cell epitopes in the OmpL protein. The B-cell epitopes predicted in the OmpL of <em>Salmonella typhimurium</em> are highly conserved among the other <em>Enterobacteriaceae</em> pathogens.</div></div><div><h3>Conclusion</h3><div>In conclusion, these conserved B-cell epitopes will vouch for the generation of heterologous humoral immune response in conferring cross protection against the <em>Enterobacteriaceae</em> pathogens and control their outbreaks across the globe.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"21 1","pages":"Article 146"},"PeriodicalIF":3.5000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10682294/pdf/","citationCount":"0","resultStr":"{\"title\":\"Mapping of conserved immunodominant epitope peptides in the outer membrane porin (Omp) L of prominent Enterobacteriaceae pathogens associated with gastrointestinal infections\",\"authors\":\"Harish Babu Kolla , Shivakiran Satyanarayan Makam , Prakash Narayana Reddy\",\"doi\":\"10.1186/s43141-023-00622-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div>Members of <em>Enterobacteriaceae</em> such as <em>Escherichia coli</em> O 157:H7, <em>Salmonella</em> sp., <em>Shigella</em> sp., <em>Klebsiella</em> sp., and <em>Citrobacter freundii</em> are responsible for the outbreak of serious foodborne illness and other mucosal infections across the globe. The outer membrane proteins (OMPs) of <em>Enterobacteriaceae</em> are highly immunogenic in eliciting immune responses against pathogens. Moreover, the OMPs are highly conserved in the <em>Enterobacteriaceae</em> family. Sequence homology in the OMPs will ensure the presence of conserved immunodominant regions with predominant epitopes. The OmpL is such an immunogen that is highly conserved among the <em>Enterobacteriaceae</em> pathogens. In this study, we performed computational analysis on the outer membrane porin (Omp) L of prominent <em>Enterobacteriaceae</em> pathogens.</div></div><div><h3>Results</h3><div>Multiple sequence and structural alignment analysis have revealed that the OmpL protein is highly conserved among the selected <em>Enterobacteriaceae</em> pathogens. This amount of sequence and structural homology uncovered the conserved antibody binding B-cell epitopes in the OmpL protein. The B-cell epitopes predicted in the OmpL of <em>Salmonella typhimurium</em> are highly conserved among the other <em>Enterobacteriaceae</em> pathogens.</div></div><div><h3>Conclusion</h3><div>In conclusion, these conserved B-cell epitopes will vouch for the generation of heterologous humoral immune response in conferring cross protection against the <em>Enterobacteriaceae</em> pathogens and control their outbreaks across the globe.</div></div>\",\"PeriodicalId\":53463,\"journal\":{\"name\":\"Journal of Genetic Engineering and Biotechnology\",\"volume\":\"21 1\",\"pages\":\"Article 146\"},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2023-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10682294/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Genetic Engineering and Biotechnology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1687157X23009769\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Genetic Engineering and Biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1687157X23009769","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
Mapping of conserved immunodominant epitope peptides in the outer membrane porin (Omp) L of prominent Enterobacteriaceae pathogens associated with gastrointestinal infections
Background
Members of Enterobacteriaceae such as Escherichia coli O 157:H7, Salmonella sp., Shigella sp., Klebsiella sp., and Citrobacter freundii are responsible for the outbreak of serious foodborne illness and other mucosal infections across the globe. The outer membrane proteins (OMPs) of Enterobacteriaceae are highly immunogenic in eliciting immune responses against pathogens. Moreover, the OMPs are highly conserved in the Enterobacteriaceae family. Sequence homology in the OMPs will ensure the presence of conserved immunodominant regions with predominant epitopes. The OmpL is such an immunogen that is highly conserved among the Enterobacteriaceae pathogens. In this study, we performed computational analysis on the outer membrane porin (Omp) L of prominent Enterobacteriaceae pathogens.
Results
Multiple sequence and structural alignment analysis have revealed that the OmpL protein is highly conserved among the selected Enterobacteriaceae pathogens. This amount of sequence and structural homology uncovered the conserved antibody binding B-cell epitopes in the OmpL protein. The B-cell epitopes predicted in the OmpL of Salmonella typhimurium are highly conserved among the other Enterobacteriaceae pathogens.
Conclusion
In conclusion, these conserved B-cell epitopes will vouch for the generation of heterologous humoral immune response in conferring cross protection against the Enterobacteriaceae pathogens and control their outbreaks across the globe.
期刊介绍:
Journal of genetic engineering and biotechnology is devoted to rapid publication of full-length research papers that leads to significant contribution in advancing knowledge in genetic engineering and biotechnology and provide novel perspectives in this research area. JGEB includes all major themes related to genetic engineering and recombinant DNA. The area of interest of JGEB includes but not restricted to: •Plant genetics •Animal genetics •Bacterial enzymes •Agricultural Biotechnology, •Biochemistry, •Biophysics, •Bioinformatics, •Environmental Biotechnology, •Industrial Biotechnology, •Microbial biotechnology, •Medical Biotechnology, •Bioenergy, Biosafety, •Biosecurity, •Bioethics, •GMOS, •Genomic, •Proteomic JGEB accepts