尿细胞学样本中过氧化物酶体增殖物激活受体γ的诊断免疫细胞染色。

IF 1.6 4区 医学 Q3 PATHOLOGY
Acta Cytologica Pub Date : 2024-01-01 Epub Date: 2023-11-26 DOI:10.1159/000535448
Shinichi Tanaka, Yasunori Tokuhara, Sanae Ariyasu, Tatsuya Morinishi, Tamami Yamamoto, Norihiro Teramoto, Eiichiro Hirakawa
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引用次数: 0

摘要

导读:尿细胞学检查是检测尿路上皮癌(UC)的常用方法,但其灵敏度不高。需要提高免疫细胞染色作为辅助方法的细胞诊断的准确性。本研究旨在确定尿细胞学中过氧化物酶体增殖物激活受体γ (PPAR-γ)免疫细胞染色在尿路上皮癌,特别是低级别尿路上皮癌(LGUC)检测中的细胞诊断价值。方法:对37例尿路上皮癌和26例良性尿路上皮癌进行PPAR-γ免疫细胞染色。在UC病例中,22例为乳头状增生型,不包括乳头状和扁平生长的混合型。所有乳头状增生类型的LGUC病例共15例。为了比较,同样的样本也进行了p53和Ki-67的免疫细胞染色。结果:37例UC患者PPAR-γ阳性25例,26例良性患者PPAR-γ阴性24例。无论组织学分级如何,22例伴有乳头状增生的UC患者中有13例PPAR-γ阳性。特别是,PPAR-γ免疫细胞染色对LGUC病例的敏感性高于其他生物标志物。对于LGUC, 10例未通过原代细胞学鉴定的病例中有4例通过PPAR-γ免疫细胞染色检测到。结论:PPAR-γ免疫细胞染色提高了尿细胞诊断的准确性。此外,PPAR-γ在尿细胞学中是一种比p53和Ki-67更有用的免疫生物标志物,而p53和Ki-67是常用的恶性细胞检测免疫生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Diagnostic Immunocytostaining with Peroxisome Proliferator-Activated Receptor-Gamma in Urine Cytology Samples.

Introduction: Urine cytology is a common method for detection of urothelial carcinoma (UC), however, is not high sensitivity. Improvement of the accuracy of cytodiagnosis using immunocytostaining as an auxiliary method is needed. This study aimed to determine the cytodiagnostic usefulness of peroxisome proliferator-activated receptor-gamma (PPAR-γ) immunocytostaining in urine cytology for the detection of UCs, particularly low-grade urothelial carcinomas (LGUC).

Methods: PPAR-γ immunocytostaining was performed for 37 UC cases and 26 benign cases. Among the UC cases, 22 cases were of the papillary proliferation type, not including the mixed type comprising both papillary and flat growth. Fifteen LGUC cases of all papillary proliferation types were included. For comparison, the same samples were also immunocytostained for p53 and Ki-67.

Results: Of the UC cases, 25 of 37 were positive for PPAR-γ, while 24 of the 26 benign cases were PPAR-γ-negative. Regardless of histological grading, 13 of the 22 UC cases with papillary proliferation were PPAR-γ-positive. In particular, PPAR-γ immunocytostaining showed higher sensitivity for LGUC cases than that of the other biomarkers. Regarding LGUC specifically, 4 of 10 cases not identified by primary cytology were detected by PPAR-γ immunocytostaining.

Conclusion: PPAR-γ immunocytostaining enhances the accuracy of urine cytodiagnosis. Furthermore, PPAR-γ is a more useful immunobiomarker in urine cytology than p53 and Ki-67, the commonly used immunobiomarkers for malignant cell detection.

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来源期刊
Acta Cytologica
Acta Cytologica 生物-病理学
CiteScore
3.70
自引率
11.10%
发文量
46
审稿时长
4-8 weeks
期刊介绍: With articles offering an excellent balance between clinical cytology and cytopathology, ''Acta Cytologica'' fosters the understanding of the pathogenetic mechanisms behind cytomorphology and thus facilitates the translation of frontline research into clinical practice. As the official journal of the International Academy of Cytology and affiliated to over 50 national cytology societies around the world, ''Acta Cytologica'' evaluates new and existing diagnostic applications of scientific advances as well as their clinical correlations. Original papers, review articles, meta-analyses, novel insights from clinical practice, and letters to the editor cover topics from diagnostic cytopathology, gynecologic and non-gynecologic cytopathology to fine needle aspiration, molecular techniques and their diagnostic applications. As the perfect reference for practical use, ''Acta Cytologica'' addresses a multidisciplinary audience practicing clinical cytopathology, cell biology, oncology, interventional radiology, otorhinolaryngology, gastroenterology, urology, pulmonology and preventive medicine.
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