市售胎牛血清及其替代品在培养肉发育中的应用分析。

Da Young Lee, Seung Hyeon Yun, Seung Yun Lee, Juhyun Lee, Ermie Jr Mariano, Seon-Tea Joo, Inho Choi, Jung Seok Choi, Gap-Don Kim, Jihyun Lee, Seong-Ho Choi, Sun Jin Hur
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引用次数: 0

摘要

胎牛血清(FBS)是一种极其重要的培养生长补充剂,约占细胞培养基成本的60%;因此,降低人造肉产业化所需的fbs获取成本势在必行。本研究试图利用废弃的牲畜副产品生产一种FBS替代品,并特别关注与FBS成分相似的产品配方,以提高有效性。然而,到目前为止,还没有研究精确地分析了FBS的商业成分,而本研究是第一个将FBS和从美国或欧洲购买的市售马血清的化学成分与我们团队开发的FBS替代品进行比较的研究。本研究通过血液、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和独立成分分析,对我们团队近3年来购买的FBS产品进行了化学成分分析。虽然已知商业FBS产品的成分和质量各不相同,但无论公司,品牌或原产国如何,我们购买的产品的FBS成分相对统一。相比之下,从三种主要家畜物种(牛、猪和鸡)中获得的FBS替代品在成分上明显存在差异,这一现象在比较FBS和不同物种之间也可以观察到。因此,为了完全取代商业FBS,需要生产一种含有相同或类似成分的比例有效的替代产品,而本研究的结果可以成为实现这一目标的踏脚石。此外,以廉价的屠宰副产品为原料生产的人造肉替代品有望最终降低养殖肉生产的单位成本。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Analysis of commercial fetal bovine serum (FBS) and its substitutes in the development of cultured meat.

Fetal bovine serum (FBS) is an extremely important culture growth supplement, accounting for approximately 60 % of cell-culture-media costs; therefore, lowering FBS-acquisition costs for the industrialization of cultured meat is imperative. This study attempted to produce an FBS substitute using discarded livestock by-products, with particular focus on formulating a product with a composition similar to that of FBS to improve effectiveness. However, to date, no study has precisely analyzed the commercial components of FBS, and this study is the first to compare the chemical composition of FBS and commercially available horse serum purchased from the United States or Europe with that of FBS substitutes developed by our team. This study analyzed the chemical composition of the FBS products purchased by our team over the past 3 years via blood, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and independent composition analyses. While the composition and quality of commercial FBS products are known to vary, the FBS composition of our purchased products was relatively uniform regardless of company, brand, or country of origin. In contrast, FBS substitutes obtained from three major livestock species (cattle, pig, and chicken) clearly exhibited differences in composition, a phenomenon that was also observed upon comparing with FBS as well as among different species. Therefore, to replace commercial FBS entirely, the production of a proportionately effective substitute product comprising an equal or similar composition is required, and the results of this study can be a steppingstone to achieving this. In addition, FBS substitutes manufactured using inexpensive slaughter by-products as raw materials are expected to ultimately reduce the unit cost of cultured meat production.

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