基于智能手机的聚二乙炔比色传感器,用于即时诊断细菌感染

Q1 Engineering
Yue Zhou , Yumeng Xue , Xubo Lin , Menglong Duan , Weili Hong , Lina Geng , Jin Zhou , Yubo Fan
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引用次数: 0

摘要

特别是在资源有限的环境中,即时检测(POCT)已成为一种很有前途的以患者为中心的分散传染病控制方法。POCT设备应价格低廉、快速、操作简单,最好不需要电源。在这里,我们开发了一个简单的细菌传感平台,可以通过智能手机操作,基于聚二乙炔(PDA)阵列膜芯片进行细菌鉴定和抗菌药物敏感性测试(AST)。每个PDA阵列根据细菌毒素在PDA-脂质组件内的仿生脂质双层上的不同作用模式,为每种细菌产生独特的彩色“指纹”图案。研究人员发现,在智能手机应用程序的帮助下,基于pda的设备可以在1.5小时内检测到低至104 CFU/mL的细菌活细胞,而传统的细菌鉴定需要几天时间。该设备还可以在4小时内用于至少两种广谱抗菌素的抗生素敏感性试验(AST),并提供抗生素敏感性和耐药性鉴定,从而选择合适的治疗方法。这种基于pda的传感平台为细菌检测提供了另一种方法,可以作为一种便携式和廉价的POCT设备,用于在资源有限的环境中快速检测细菌感染。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Smartphone-based polydiacetylene colorimetric sensor for point-of-care diagnosis of bacterial infections

Smartphone-based polydiacetylene colorimetric sensor for point-of-care diagnosis of bacterial infections

The rapid progress in point-of-care testing (POCT) has become a promising decentralized patient-centered approach for the control of infectious diseases, especially in resource-limited settings. POCT devices should be inexpensive, rapid, simple operation and preferably require no power supply. Here, we developed a simple bacterial sensing platform that can be operated by a smartphone for bacteria identification and antimicrobial susceptibility testing (AST) based on using a polydiacetylene (PDA) arrayed membrane chip. Each PDA array produced a unique color ‘fingerprint’ pattern for each bacteria based on different modes of action of toxins from bacteria on biomimetic lipid bilayers within PDA-lipid assemblies. We show that the PDA-based device can detect viable cells of bacteria as low as 104 ​CFU/mL within 1.5 ​h compared with several days of conventional bacterial identification, with the aid of a smartphone app. The device can also be used for an antimicrobial susceptibility test (AST) for at least two broad-spectrum antimicrobials within 4 ​h and provide identification of antimicrobial susceptibility and resistance, enabling the selection of appropriate therapies. This PDA-based sensing platform provides an alternative way for bacterial detection and could be used as a portable and inexpensive POCT device for the rapid detection of bacterial infection in limited-resource settings.

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来源期刊
Smart Materials in Medicine
Smart Materials in Medicine Engineering-Biomedical Engineering
CiteScore
14.00
自引率
0.00%
发文量
41
审稿时长
48 days
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