具有GABA受体的外毛细胞亚群。

P K Plinkert, H Möhler, H P Zenner
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引用次数: 16

摘要

在哺乳动物耳蜗中,橄榄耳蜗神经支配已被假定为调节主动机械过程。组织化学研究表明,这些主要终止于外毛细胞(ohc)的传出神经亚群是γ -氨基丁酸(GABA)能神经。通过两种单克隆抗体,我们能够可视化gabaa受体在分离的感觉细胞基极的免疫反应性。GABAA受体的两个亚基,α -亚基和β -亚基,已知形成跨膜GABA/苯二氮卓类受体复合物,并存在于ohc上。此外,这些抑制受体在耳蜗的尖匝中数量更多,这是区分耳蜗尖匝和底匝的另一个标准。这些结果支持了OHCs基极轴体突触亚群释放抑制性神经递质GABA进入突触间隙的概念。递质与这些新观察到的突触下受体结合后,可能会发生OHC运动的改变和随后基底膜运动的调节。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A subpopulation of outer hair cells possessing GABA receptors with tonotopic organization.

The olivocochlear innervation has been postulated to regulate active mechanical processes in the mammalian cochlea. Histochemical studies led to the suggestion that a subpopulation of these efferent nerves, which predominantly terminate on outer hair cells (OHCs), are gamma-aminobutyric acid (GABA)-ergic. By means of two monoclonal antibodies, we were able to visualize GABAA-receptor immunoreactivity at the basal pole of isolated sensory cells. Both subunits of the GABAA receptor, the alpha- and beta-subunit, are known to form the transmembranous GABA/benzodiazepine-receptor complex and were present on OHCs. In addition, these inhibitory receptors were more numerous in the apical turns of the cochlea, indicating another criterion for distinguishing the apical from basal turns of the cochlea. These results support the concept that a subpopulation of axosomatic synapses at the basal pole of OHCs liberate the inhibitory neurotransmitter GABA into the synaptic cleft. Binding of the transmitter to these newly observed subsynaptic receptors is possibly followed by a change in OHC motility and a subsequent modulation of the movement of the basilar membrane.

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