[G1精原染色体]。

E Bustos-Obregón
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引用次数: 0

摘要

Chalones是细胞增殖的生理抑制剂,在细胞周期的G1或G2期起作用。它们已被描述为多种组织,包括精原上皮。对大鼠G1精原细胞chalone的体内和体外表征表明,它是一种糖蛋白,热不稳定,分子量在5000 D以下,具有组织特异性而非物种特异性,在生理pH下具有活性,其细胞作用机制由环AMP介导。该物质的来源是原代精母细胞到圆形精母细胞的精子发生分化细胞。目标细胞是A型(可能只有A0型)精原细胞。这种抑制作用是通过减少睾丸DNA对h3 -胸腺嘧啶的吸收来测量的,它不依赖于睾丸类固醇、支持细胞产物(抑制素或类似物质),也不依赖于下丘脑-垂体-性腺轴,因为它发生在体外。在小鼠中,G1精原染色体的生物半衰期(体内)约为14小时。在小鼠精子发生的整个过程中,长期给药不会改变生精动力学,也不会导致无精子症。G1精原chalone的生物学效应可以通过兔免疫血清对抗部分纯化的大鼠睾丸提取物(chalone的来源)在体外抵消。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[G1 spermatogonial chalone].

Chalones are physiological inhibitors of cell proliferation that act either at the G1 or G2 phase of the cell cycle. They have been described for a variety of tissues, including the seminiferous epithelium. In vivo and in vitro characterization of rat G1 spermatogonial chalone demonstrate that it is a glycoprotein, heat-labile, molecular weight under 5,000 D, tissue specific but not species-specific, active at physiological pH, with a mechanism of cell action mediated by cyclic AMP. The origin of the substance are the differentiated cells of the spermatogenesis from primary spermatocytes up to round spermatids. The target cells are the type A (and perhaps only the A0) spermatogonia. The inhibitory effect, measured as a decrease in the uptake of H3-thymidine into testicular DNA, is not dependent on testicular steroids, Sertoli cell products (inhibin or the like) nor on the hypothalamic-hypophyseal-gonadal axis, since it occurs in vitro. In the mouse, the biological half-life (in vivo) of the G1 spermatogonial chalone is around 14 hs. Chronic administration for the entire length of mouse spermatogenesis does not alter spermatogenic kinetics nor does it result in azoospermia. The biological effect of the G1 spermatogonial chalone can be counteracted in vitro by means of an immune rabbit serum raised against a partially purified rat testicular extract (source of the chalone).

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