{"title":"与非核糖体肽合成酶基因连接的mmtN细菌生产二甲基磺酰丙酸。","authors":"Jinmei Li, Jonathan Todd, Zhisheng Yu","doi":"10.1080/09593330.2023.2283792","DOIUrl":null,"url":null,"abstract":"<p><p>Dimethylsulfoniopropionate (DMSP) is a vital sulfur-containing compound with worldwide significance, serving as the primary precursor for dimethyl sulfide (DMS), a volatile sulfur compound that plays a role in atmospheric chemistry and influences the Earth's climate on a global scale. The study investigated the ability of four bacterial strains, namely <i>Acidimangrovimonas sediminis</i> MS2-2 (MS2-2), <i>Hartmannibacter diazotrophicus</i> E18<sup>T</sup> (E18<sup>T</sup>), <i>Rhizobium lusitanum</i> 22705 (22705), and <i>Nitrospirillum iridis</i> DSM22198 (DSM22198), to produce and degrade DMSP. These strains were assessed for their DMSP synthesis ability with the <i>mmtN</i> linked to non-ribosomal peptide synthase (NRPS) gene. The results showed that MS2-2, and E18<sup>T</sup> bacteria, which contained the <i>mmtN</i> but not linked to an NRPS gene, increased DMSP production with increasing salinity. The highest production of DMSP was achieved at 25 PSU when either methionine was added or low nitrogen conditions were present, yielding 1656.03 ± 41.04 and 265.59 ± 9.17 nmol/mg protein, respectively, and subsequently under the conditions of methionine addition or low nitrogen, both strains reached their maximum DMSP production at 25 PSU. Furthermore, the strains MS2-2, E18<sup>T</sup>, and 22705 with the <i>mmtN</i> gene but not linked to an NRPS gene were found to be involved in DMS production. This research contributes to the understanding of the genes involved in DMSP biosynthesis in bacteria that produce DMSP.</p>","PeriodicalId":12009,"journal":{"name":"Environmental Technology","volume":null,"pages":null},"PeriodicalIF":2.2000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The production of dimethylsulfoniopropionate by bacteria with <i>mmtN</i> linked to non-ribosomal peptide synthase gene.\",\"authors\":\"Jinmei Li, Jonathan Todd, Zhisheng Yu\",\"doi\":\"10.1080/09593330.2023.2283792\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Dimethylsulfoniopropionate (DMSP) is a vital sulfur-containing compound with worldwide significance, serving as the primary precursor for dimethyl sulfide (DMS), a volatile sulfur compound that plays a role in atmospheric chemistry and influences the Earth's climate on a global scale. The study investigated the ability of four bacterial strains, namely <i>Acidimangrovimonas sediminis</i> MS2-2 (MS2-2), <i>Hartmannibacter diazotrophicus</i> E18<sup>T</sup> (E18<sup>T</sup>), <i>Rhizobium lusitanum</i> 22705 (22705), and <i>Nitrospirillum iridis</i> DSM22198 (DSM22198), to produce and degrade DMSP. These strains were assessed for their DMSP synthesis ability with the <i>mmtN</i> linked to non-ribosomal peptide synthase (NRPS) gene. The results showed that MS2-2, and E18<sup>T</sup> bacteria, which contained the <i>mmtN</i> but not linked to an NRPS gene, increased DMSP production with increasing salinity. The highest production of DMSP was achieved at 25 PSU when either methionine was added or low nitrogen conditions were present, yielding 1656.03 ± 41.04 and 265.59 ± 9.17 nmol/mg protein, respectively, and subsequently under the conditions of methionine addition or low nitrogen, both strains reached their maximum DMSP production at 25 PSU. Furthermore, the strains MS2-2, E18<sup>T</sup>, and 22705 with the <i>mmtN</i> gene but not linked to an NRPS gene were found to be involved in DMS production. This research contributes to the understanding of the genes involved in DMSP biosynthesis in bacteria that produce DMSP.</p>\",\"PeriodicalId\":12009,\"journal\":{\"name\":\"Environmental Technology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2024-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environmental Technology\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://doi.org/10.1080/09593330.2023.2283792\",\"RegionNum\":4,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/11/24 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Technology","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1080/09593330.2023.2283792","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/11/24 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
The production of dimethylsulfoniopropionate by bacteria with mmtN linked to non-ribosomal peptide synthase gene.
Dimethylsulfoniopropionate (DMSP) is a vital sulfur-containing compound with worldwide significance, serving as the primary precursor for dimethyl sulfide (DMS), a volatile sulfur compound that plays a role in atmospheric chemistry and influences the Earth's climate on a global scale. The study investigated the ability of four bacterial strains, namely Acidimangrovimonas sediminis MS2-2 (MS2-2), Hartmannibacter diazotrophicus E18T (E18T), Rhizobium lusitanum 22705 (22705), and Nitrospirillum iridis DSM22198 (DSM22198), to produce and degrade DMSP. These strains were assessed for their DMSP synthesis ability with the mmtN linked to non-ribosomal peptide synthase (NRPS) gene. The results showed that MS2-2, and E18T bacteria, which contained the mmtN but not linked to an NRPS gene, increased DMSP production with increasing salinity. The highest production of DMSP was achieved at 25 PSU when either methionine was added or low nitrogen conditions were present, yielding 1656.03 ± 41.04 and 265.59 ± 9.17 nmol/mg protein, respectively, and subsequently under the conditions of methionine addition or low nitrogen, both strains reached their maximum DMSP production at 25 PSU. Furthermore, the strains MS2-2, E18T, and 22705 with the mmtN gene but not linked to an NRPS gene were found to be involved in DMS production. This research contributes to the understanding of the genes involved in DMSP biosynthesis in bacteria that produce DMSP.
期刊介绍:
Environmental Technology is a leading journal for the rapid publication of science and technology papers on a wide range of topics in applied environmental studies, from environmental engineering to environmental biotechnology, the circular economy, municipal and industrial wastewater management, drinking-water treatment, air- and water-pollution control, solid-waste management, industrial hygiene and associated technologies.
Environmental Technology is intended to provide rapid publication of new developments in environmental technology. The journal has an international readership with a broad scientific base. Contributions will be accepted from scientists and engineers in industry, government and universities. Accepted manuscripts are generally published within four months.
Please note that Environmental Technology does not publish any review papers unless for a specified special issue which is decided by the Editor. Please do submit your review papers to our sister journal Environmental Technology Reviews at http://www.tandfonline.com/toc/tetr20/current