反式铁力内酯对HepG2细胞和小鼠肝脏极低密度脂蛋白分泌的抑制作用。

IF 2.5 4区 医学 Q3 CHEMISTRY, MEDICINAL
Akifumi Nagatomo, Mamiko Kohno, Hirosato Kawakami, Yoshiaki Manse, Toshio Morikawa
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引用次数: 0

摘要

一种酰基化的黄酮醇苷,反式tiliroside(1),存在于不同草药的某些部分,包括Rosa canina(蔷薇科)的种子。以往对化合物1的研究主要集中在甘油三酯(TG)代谢方面,包括其抗肥胖和降低细胞内TG的作用。本研究利用人肝细胞癌源性HepG2细胞和小鼠研究了化合物1对胆固醇(CHO)代谢的影响。化合物1降低HepG2细胞的CHO分泌,甲羟戊酸呈浓度依赖性增强CHO分泌,并降低载脂蛋白B (apoB)-100的分泌,载脂蛋白B是极低密度脂蛋白(VLDL)的标志。化合物1还抑制微粒体甘油三酯转移蛋白的活性,该蛋白介导肝脏中胆固醇和甘油三酯形成VLDL。在体内,化合物1抑制Triton wr -1339诱导的TG在禁食小鼠血液中的积累,并维持低水平的apoB-100。这些结果表明,化合物1可以抑制肝脏以VLDL形式分泌CHO,并有可能用于预防血脂异常。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Inhibitory effect of trans-tiliroside on very low-density lipoprotein secretion in HepG2 cells and mouse liver

Inhibitory effect of trans-tiliroside on very low-density lipoprotein secretion in HepG2 cells and mouse liver

An acylated flavonol glycoside, trans-tiliroside (1), is found in certain parts of different herbs, including the seeds of Rosa canina (Rosaceae). Previous studies on compound 1 have focused on triglyceride (TG) metabolism, including its anti-obesity and intracellular TG reduction effects. In the present study, the effects of compound 1 on cholesterol (CHO) metabolism were investigated using human hepatocellular carcinoma-derived HepG2 cells and mice. Compound 1 decreased CHO secretion in HepG2 cells, which was enhanced by mevalonate in a concentration-dependent manner and decreased the secretion of apoprotein B (apoB)-100, a marker of very low-density lipoprotein (VLDL). Compound 1 also inhibited the activity of microsomal triglyceride transfer proteins, which mediate VLDL formation from cholesterol and triglycerides in the liver. In vivo, compound 1 inhibited the accumulation of Triton WR-1339-induced TG in the blood of fasted mice and maintained low levels of apoB-100. These results suggest that compound 1 inhibits the secretion of CHO as VLDL from the liver and has the potential for use for the prevention of dyslipidemia.

Graphical abstract

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来源期刊
CiteScore
6.90
自引率
3.00%
发文量
79
审稿时长
1.7 months
期刊介绍: The Journal of Natural Medicines is an international journal publishing original research in naturally occurring medicines and their related foods and cosmetics. It covers: -chemistry of natural products -biochemistry of medicinal plants -pharmacology of natural products and herbs, including Kampo formulas and traditional herbs -botanical anatomy -cultivation of medicinal plants. The journal accepts Original Papers, Notes, Rapid Communications and Natural Resource Letters. Reviews and Mini-Reviews are generally invited.
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