[大鼠胆道胆固醇高分泌状态下脂蛋白分解代谢的表征]。

M P Marzolo, F Nervi
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引用次数: 0

摘要

肝脏是体内固醇排泄的主要途径。此外,大部分血清脂蛋白在肝脏中分解代谢。已知薯蓣皂苷元和豆类饮食可显著诱导胆汁胆固醇分泌。在这一系列的研究中,我们描述了几种脂蛋白颗粒在喂食薯蓣皂苷元或富含豆类的饮食中的分解代谢(这些动物的胆道胆固醇输出增加了300%以上)。用125I标记人低密度脂蛋白(hLDL)和大鼠无载脂蛋白e (rHDL)。用胆固醇-(3H)-油酸酯标记大鼠乳糜微粒。豆食大鼠的hLDL清除率从381 +/- 39增加到628 +/- 44(微升/小时× 100 g体重量)(p < 0.005)。半衰期(t1/2)由12.4 +/- 1降至9.8 +/- 0.7 h (p < 0.005)。黄芪皂苷元喂养动物的rHDL - apo-E清除率从579 +/- 8增加到680 +/- 36(微升/小时× 100 g体重)(p < 0.05)。t1/2由8.2 +/- 0.7 h显著降低至7.0 +/- 0.3 h (p < 0.05)。相反,乳糜微粒清除率和t1/2不受薯蓣皂苷元或豆类饲料的影响。这些结果与假设一致,即B/E和A-I受体的数量和/或亲和力增加,而不是E受体。这些研究表明肝脏胆固醇的胆道通路和窦道通路之间存在着功能上的相互关系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Characterization of lipoprotein catabolism in biliary cholesterol hypersecretion conditions in rats].

The liver represents the principal pathway for sterol excretion from the organism. In addition, the major proportion of serum lipoproteins is catabolized in the liver. It is known that diosgenin and bean diet markedly induce biliary cholesterol output. In this series of studies we characterized the catabolism of several lipoprotein particles in animals fed diosgenin or a bean-rich diet (biliary cholesterol output is increased greater than 300% in these animals). Human low density lipoprotein (hLDL) and rat high density lipoprotein apo-E free (rHDL) were labeled with 125I. Rat chylomicrons were labeled with cholesterol-(3H)-oleate. hLDL clearance increased from 381 +/- 39 to 628 +/- 44 (microliters/h x 100 g body wt) (p less than 0.005) in bean-fed rats. The half life (t1/2) decreased from 12.4 +/- 1 to 9.8 +/- 0.7 h (p less than 0.005) in these rats. The clearance of rHDL apo-E free increased from 579 +/- 8 to 680 +/- 36 (microliters/h x 100 g body wt) (p less than 0.05) in diosgenin-fed animals. The t1/2 significantly decreased from 8.2 +/- 0.7 h to 7.0 +/- 0.3 h (p less than 0.05) in these rats. In contrast, chylomicron clearance and t1/2 were not modified by the diosgenin or bean diets. These results are consistent with the hypothesis that the number and/or affinity of the B/E and A-I receptors, but not E receptors are increased in rats fed biliary cholesterol hypersecretory diets. These studies suggests the presence of a functional inter-relationship between the biliary and sinusoidal pathways of hepatic cholesterol.

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