姜水提物和运动通过上调血清Sirtuin-1改善果糖诱导的2型糖尿病大鼠胰岛素抵抗的作用

None Seher Naeem, Muniza Saeed, Jawaria Ilyas, Asma Ashraf, Madiha Akram, Ruqayyah Nazir
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引用次数: 0

摘要

导论:2型糖尿病(T2DM)在发展中国家的发病率越来越高,迫切需要寻找新的治疗策略,不仅针对其潜在的病理过程,而且安全且经济有效。目的:探讨补充生姜、运动及两者联合对果糖诱导的2型糖尿病大鼠胰岛素抵抗(IR)和血清sirtuin 1 (SIRT1)水平的影响。材料&方法:本试验于2021年1月至2021年6月在巴基斯坦拉合尔研究生医学研究所动物实验室进行随机对照试验,30只大鼠随机分为5组,每组6只大鼠。1组(正常对照组)给予正常鼠粮。2组、3组和4组小鼠给予25%果糖饮食诱导糖尿病,随后,2组作为糖尿病/阳性对照(PC),继续只给予25%果糖饮食。第3组给予生姜水提液(GE),第4组给予游泳运动(EX),第5组给予二者联合(GE+EX),为期8周。在PGMI药理学实验室制备生姜水提物,将50 gm鲜姜与75ml 0.9% NaCl混合,过滤三次。将所得滤液以2000 rpm的转速离心10分钟。用NaCl使清上清部分达到100 ml标记,浓度为500 mg/ml,用于口服。数据输入和分析使用SPSS version 26, p值为0.05认为显著。结果:本研究结果显示,PC组在补充25%果糖的情况下发展为T2DM,导致IR水平显著升高,同时血清SIRT1水平显著降低。与糖尿病组相比,姜水提取物组和运动组的IR均显著降低,这与血清SIRT1水平显著升高有关(p<0.05)。然而,最显著的效果出现在联合用药组,IR水平最低,SIRT1水平显著升高(p < 0.05)。结论:生姜水提取物补充和运动训练单独或联合使用,通过对血清SIRT1的积极影响,有可能显著改善T2DM患者的IR。然而,联合组的效果最明显,反映了两种干预措施的潜在协同效应。生姜补充剂和运动可以作为抗糖尿病药物的更安全、更经济、更自然的辅助手段,从而降低其潜在的有害副作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Ameliorating Effect of Aqueous Ginger Extract And Exercise On Insulin Resistance in Fructose Induced Type 2 Diabetic Rats Through Upregulation of Serum Sirtuin-1
Introduction:The mounting prevalence of type 2 diabetes mellitus (T2DM) in developing countries calls for an immediate need to find novel treatment strategies that not only target its underlying pathological process but are also safe and cost effective. Aims &Objectives:To determine the effect of ginger supplementation, exercise and their combination on insulin resistance (IR) and levels of serum sirtuin 1 (SIRT1) in fructose induced type 2 diabetic rat model. Material &Methods:This randomized controlled trial was conducted at animal laboratory of Postgraduate Medical Institute Lahore, Pakistan, from January 2021 to June 2021, in which thirty rats were randomly allocated to five groups with six rats in each.Rats belonging to group1 (Normal Control) were given normal rat chow diet. Diabetes was induced in groups 2, 3 and 4 by administering 25% fructose diet, following which, group 2 was reserved as diabetic/positive control (PC) and continued to receive 25 %fructose diet only. Animals of group 3 received aqueous ginger extract (GE), group 4 underwent swimming exercise (EX) and group 5 received their combination (GE+EX) for 8 weeks. Aqueous ginger extract was prepared in the Pharmacology lab at PGMI where 50 gm of fresh ginger was blended with 75ml of 0.9% NaCl and filtered thrice. The obtained filtrate was centrifuged at a speed of 2000 rpm for ten minutes. The clear supernatant fraction was made to reach 100 ml mark using NaCl resulting in a concentration of 500 mg/ml which was used for oral administration. Data was entered and analyzed using SPSS version 26, a p-value of ?0.05 was considered significant. Results:The results of the current study showed development of T2DM with 25% fructose supplementation in PC group, resulting in significantly high level of IR along with a significant reduction in levels of serum SIRT1. Aqueousginger extract group as well as the exercise group, both individually showed a significant reduction in IR as compared to the diabetic group and this was associated with significantly increased level of serum SIRT1 (p<0.05). However, most pronounced effect was seen in the combination group having lowest level of IR associated with a statistically significant increase in SIRT1 levels (p<0.05). Conclusion: Aqueousginger extract supplementation and exercise training, alone and in combination have the potential to significantly ameliorate IR in T2DM through its positive influence on serum SIRT1.However, the combination group has the most pronounced effect reflecting a potential synergistic effect of both interventions. Ginger supplementation and exercise may be introduced as safer, cost effective and natural adjunct to anti diabetic drugs hence lowering their potentially harmful side effects.
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