{"title":"雪山荨麻疹提取物对解冻后山羊精液活力及其他特性的特殊抗氧化作用","authors":"K. AL-JAF, C. OMAR, A. VAZIRY","doi":"10.15666/aeer/2105_38653885","DOIUrl":null,"url":null,"abstract":". This study was carried out to examine the effects of Urtica dioica extract on goat cryopreserved semen quality. The ejaculates of Markhoz goats (n = 12) were collected by an electro-ejaculator. The semen was diluted and supplemented with different concentration of aqueous or hydroalcoholic Urtica dioica extract of aerial or root parts as follows: aerial-aqueous (AA1 5μg/ml , AA2 15μg/ml and AA3 45μg/ml ); aerial-ethanolic (AE1 5μg/ml , AE2 15μg/ml and AE3 45μg/ml ); root aqueous (RA1 5μg/ml , RA2 15μg/ml and RA3 45μg/ml ); root-ethanolic (RE1 5μg/ml , RE 215μg/ml and RE3 45μg/ml ) and control (CCC 0mg/ml ) without additives. After thawing, sperm samples were subjected to viability, acrosome integrity, plasma membrane integrity, motility, sperm chromatin dispersion (SCD) and concentration of malondialdehyde (MDA) assays. Addition of AA1 5μg/ml to the extender significantly (P < 0.05) improved progressive motility, viability, plasma membrane integrity, acrosome integrity, and DNA integrity of the semen samples. Sperm viability and plasma membrane integrity in aqueous extract (both aerial & root parts)-supplemented extenders were relatively higher, as compared to the control. Moreover, a significant decrease in MDA concentration was detected in semen supplemented with Urtica dioica extract treatments. In conclusion, the results indicate that 5μg/ml of aqueous extract of Urtica dioica aerial part improved sperm quality and could be recommended as an additive to the extender for cryopreservation. This study shows a novel approach in goat semen cryopreservation due to the remarkable influence of Urtica dioica on post-thawed sperm parameters.","PeriodicalId":7975,"journal":{"name":"Applied Ecology and Environmental Research","volume":"97 1","pages":"0"},"PeriodicalIF":0.6000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"EXCEPTIONAL ANTIOXIDANT EFFECT OF URTICA DIOICA EXTRACT ON MOTILITY AND SOME OTHER POST THAWED GOAT SEMEN CHARACTERISTICS\",\"authors\":\"K. AL-JAF, C. OMAR, A. VAZIRY\",\"doi\":\"10.15666/aeer/2105_38653885\",\"DOIUrl\":null,\"url\":null,\"abstract\":\". This study was carried out to examine the effects of Urtica dioica extract on goat cryopreserved semen quality. The ejaculates of Markhoz goats (n = 12) were collected by an electro-ejaculator. The semen was diluted and supplemented with different concentration of aqueous or hydroalcoholic Urtica dioica extract of aerial or root parts as follows: aerial-aqueous (AA1 5μg/ml , AA2 15μg/ml and AA3 45μg/ml ); aerial-ethanolic (AE1 5μg/ml , AE2 15μg/ml and AE3 45μg/ml ); root aqueous (RA1 5μg/ml , RA2 15μg/ml and RA3 45μg/ml ); root-ethanolic (RE1 5μg/ml , RE 215μg/ml and RE3 45μg/ml ) and control (CCC 0mg/ml ) without additives. After thawing, sperm samples were subjected to viability, acrosome integrity, plasma membrane integrity, motility, sperm chromatin dispersion (SCD) and concentration of malondialdehyde (MDA) assays. Addition of AA1 5μg/ml to the extender significantly (P < 0.05) improved progressive motility, viability, plasma membrane integrity, acrosome integrity, and DNA integrity of the semen samples. Sperm viability and plasma membrane integrity in aqueous extract (both aerial & root parts)-supplemented extenders were relatively higher, as compared to the control. Moreover, a significant decrease in MDA concentration was detected in semen supplemented with Urtica dioica extract treatments. In conclusion, the results indicate that 5μg/ml of aqueous extract of Urtica dioica aerial part improved sperm quality and could be recommended as an additive to the extender for cryopreservation. This study shows a novel approach in goat semen cryopreservation due to the remarkable influence of Urtica dioica on post-thawed sperm parameters.\",\"PeriodicalId\":7975,\"journal\":{\"name\":\"Applied Ecology and Environmental Research\",\"volume\":\"97 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.6000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Applied Ecology and Environmental Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15666/aeer/2105_38653885\",\"RegionNum\":4,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"ECOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Ecology and Environmental Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15666/aeer/2105_38653885","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ECOLOGY","Score":null,"Total":0}
EXCEPTIONAL ANTIOXIDANT EFFECT OF URTICA DIOICA EXTRACT ON MOTILITY AND SOME OTHER POST THAWED GOAT SEMEN CHARACTERISTICS
. This study was carried out to examine the effects of Urtica dioica extract on goat cryopreserved semen quality. The ejaculates of Markhoz goats (n = 12) were collected by an electro-ejaculator. The semen was diluted and supplemented with different concentration of aqueous or hydroalcoholic Urtica dioica extract of aerial or root parts as follows: aerial-aqueous (AA1 5μg/ml , AA2 15μg/ml and AA3 45μg/ml ); aerial-ethanolic (AE1 5μg/ml , AE2 15μg/ml and AE3 45μg/ml ); root aqueous (RA1 5μg/ml , RA2 15μg/ml and RA3 45μg/ml ); root-ethanolic (RE1 5μg/ml , RE 215μg/ml and RE3 45μg/ml ) and control (CCC 0mg/ml ) without additives. After thawing, sperm samples were subjected to viability, acrosome integrity, plasma membrane integrity, motility, sperm chromatin dispersion (SCD) and concentration of malondialdehyde (MDA) assays. Addition of AA1 5μg/ml to the extender significantly (P < 0.05) improved progressive motility, viability, plasma membrane integrity, acrosome integrity, and DNA integrity of the semen samples. Sperm viability and plasma membrane integrity in aqueous extract (both aerial & root parts)-supplemented extenders were relatively higher, as compared to the control. Moreover, a significant decrease in MDA concentration was detected in semen supplemented with Urtica dioica extract treatments. In conclusion, the results indicate that 5μg/ml of aqueous extract of Urtica dioica aerial part improved sperm quality and could be recommended as an additive to the extender for cryopreservation. This study shows a novel approach in goat semen cryopreservation due to the remarkable influence of Urtica dioica on post-thawed sperm parameters.
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