长春花中长春胺的高效液相色谱定量测定方法的建立与验证。叶萃取精华

Q4 Pharmacology, Toxicology and Pharmaceutics

INDIAN DRUGS Pub Date : 2023-10-28 DOI:10.53879/id.60.10.14128

Christina Viju, Sneha A. Agrawal, Aruna P. Jadhav

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引用次数: 0

摘要

Catharanthus roseus(夹竹桃科)，俗称长春花，是一种多年生植物，主要分布在南亚。长春花富含吲哚类生物碱。本研究工作包括用HPTLC对吲哚类生物碱长春胺进行开发和验证。以氯仿、丙酮、甲酸为洗脱液，以5:1:0.5 V/V/V的比例在波长222 nm下进行色谱分离。该方法对长春花胺的分离效果较好，Rf值为0.33±0.02。根据回归分析数据，长春胺在200 ~ 1000 ng spot-1范围内呈良好的线性关系，相关系数(R2)为0.9957。遵循ICH Q2 (R1)指南进行验证。

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DEVELOPMENT AND VALIDATION OF A NOVEL HPTLC METHOD FOR QUANTITATIVE ESTIMATION OF VINCAMINE FROM CATHARANTHUS ROSEUS LINN. LEAVES EXTRACT

Catharanthus roseus (Apocynaceae), commonly known as periwinkle, is a perennial plant that is mostly found in Southern Asia. Periwinkle is rich in indole alkaloids. This research work consists of the development and validation of one of the indole alkaloids, vincamine, by HPTLC. The chromatographic separation was achieved by using eluent chloroform, acetone, and formic acid in the ratio of 5:1:0.5 V/V/V at wavelength 222 nm. With an Rf value of 0.33±0.02, this method showed good separation of vincamine in the extract. Vincamine in the range of 200-1000 ng spot-1 showed a satisfactory linear relationship according to the regression analysis data, and the correlation coefficient (R2 ) was found to be 0.9957. ICH Q2 (R1) guidelines were followed for validation.

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来源期刊

INDIAN DRUGS Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science

CiteScore

0.30

自引率

0.00%

发文量