百里香叶粉水提物对昆虫幼虫α -淀粉酶抑制作用的研究

Q4 Medicine
Sarah Raad Shaker, Ibtehaj Mustafa Hakeem, Hasan Moamin Lilo
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引用次数: 1

摘要

本研究包括评价百里香叶粉水提物对昆虫幼虫(Trogoderma granarium)产生的α -淀粉酶的抑制作用,并利用高效液相色谱技术研究其中最重要的活性成分,了解其对α -淀粉酶的生物学效应。确定了最佳的温度和pH条件来测定抑制剂对酶的活性和稳定性。HPLC结果表明百里香水提物中单宁含量较高,为9微克/毫升。化学试验表明,百里香叶中含有皂苷、黄酮类化合物、苷类化合物、树脂和生物碱。结果表明,水冻干法提取率最高,为68%,且不加热也能有效保存活性成分。从研究结果可以看出,当使用浓度为10%时,对酶的抑制效果最好,这意味着抑制剂的浓度越高,其有效性越高。通过对各种因素的研究,我们发现最佳的缓蚀温度为30℃。PH值在PH = 7时。本研究认为,利用百里香提取物抑制α -淀粉酶的可能性是由昆虫幼虫(Trogoderma granarium)产生的。关键词:a-淀粉酶,谷物原皮,百里香,冻干,高效液相色谱
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Study of the inhibitory effect of aqueous extract of thyme leaf powder on alpha-amylase enzyme produced by insect larvae (Trogoderma granarium)
The study included evaluating the effectiveness of aqueous extract of Thyme leaf powder on the inhibition of the alpha-amylase enzyme produced by insect larvae (Trogoderma granarium), as well as studying the most important active compounds to know their biological effect on the enzyme by using HPLC technology. Optimum conditions of temperature and pH were determined to measure the inhibitor activity and stability toward the enzyme. The results of HPLC cleared that the thyme aqueous extract had a high level of tannin, 9 micrograms/ml. The chemical tests for this study indicated that thyme leaves contain saponins, flavonoids, glycosides, resins and alkaloids. The results showed that the aqueous lyophilization process gave the best extraction rate of 68% and also preserved the active compounds without heat. It was noted from the study results that the best inhibition of the enzyme was when using a concentration of 10%, which means that the higher the concentration of the inhibitor is, the higher its effectiveness. By studying the various factors, we find that the best temperature for inhibition is 30 °C. As for PH, it was at pH = 7. It has been concluded in this study that the possibility of using the extract of thyme to inhibit the alpha-amylase enzyme is produced by Insect larvae (Trogoderma granarium) as a paradigm. Keywords: a-amylase, Trogoderma granarium, thyme, lyophilization, HPLC
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来源期刊
Revista Bionatura
Revista Bionatura Medicine-Epidemiology
CiteScore
0.50
自引率
0.00%
发文量
144
审稿时长
8 weeks
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