Emine E. Alieva , Muniver T. Gafarova , Eugene I. Bondarenko , Igor A. Dovgan , Irina I. Osiptchuk , Marina E. Eremeeva
{"title":"在克里米亚使用焦痂拭子 DNA 诊断 Conorii 亚种 Conorii 立克次体感染：病例报告","authors":"Emine E. Alieva , Muniver T. Gafarova , Eugene I. Bondarenko , Igor A. Dovgan , Irina I. Osiptchuk , Marina E. Eremeeva","doi":"10.1016/j.imj.2023.09.004","DOIUrl":null,"url":null,"abstract":"<div><p>Mediterranean spotted fever (MSF) has been diagnosed clinically in the Crimean Peninsula since the 1930s. We describe the recent illness of an elderly patient from Crimea who had developed a classic triad of MSF symptoms consisting of fever, maculopapular rash, and eschar. Clinical diagnosis of rickettsiosis was confirmed using real-time PCR and sequencing of 4 <em>Rickettsia</em> protein genes. The strain causing clinical illness was characterized as <em>Rickettsia conorii</em> subspecies <em>conorii</em> Malish 7. This report corroborates the utility of eschar swab material as a source of DNA for PCR-based diagnostics that enables timely patient treatment and management<em>.</em></p></div>","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"2 4","pages":"Pages 338-342"},"PeriodicalIF":0.0000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772431X23000552/pdfft?md5=5e6e114e348e4112265cef7e4de1e575&pid=1-s2.0-S2772431X23000552-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Use of eschar swab DNA to diagnose Rickettsia conorii subspecies conorii infection in Crimea: A case report\",\"authors\":\"Emine E. Alieva , Muniver T. Gafarova , Eugene I. Bondarenko , Igor A. Dovgan , Irina I. Osiptchuk , Marina E. Eremeeva\",\"doi\":\"10.1016/j.imj.2023.09.004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Mediterranean spotted fever (MSF) has been diagnosed clinically in the Crimean Peninsula since the 1930s. We describe the recent illness of an elderly patient from Crimea who had developed a classic triad of MSF symptoms consisting of fever, maculopapular rash, and eschar. Clinical diagnosis of rickettsiosis was confirmed using real-time PCR and sequencing of 4 <em>Rickettsia</em> protein genes. The strain causing clinical illness was characterized as <em>Rickettsia conorii</em> subspecies <em>conorii</em> Malish 7. This report corroborates the utility of eschar swab material as a source of DNA for PCR-based diagnostics that enables timely patient treatment and management<em>.</em></p></div>\",\"PeriodicalId\":100667,\"journal\":{\"name\":\"Infectious Medicine\",\"volume\":\"2 4\",\"pages\":\"Pages 338-342\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2772431X23000552/pdfft?md5=5e6e114e348e4112265cef7e4de1e575&pid=1-s2.0-S2772431X23000552-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Infectious Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2772431X23000552\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Infectious Medicine","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2772431X23000552","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Use of eschar swab DNA to diagnose Rickettsia conorii subspecies conorii infection in Crimea: A case report
Mediterranean spotted fever (MSF) has been diagnosed clinically in the Crimean Peninsula since the 1930s. We describe the recent illness of an elderly patient from Crimea who had developed a classic triad of MSF symptoms consisting of fever, maculopapular rash, and eschar. Clinical diagnosis of rickettsiosis was confirmed using real-time PCR and sequencing of 4 Rickettsia protein genes. The strain causing clinical illness was characterized as Rickettsia conorii subspecies conorii Malish 7. This report corroborates the utility of eschar swab material as a source of DNA for PCR-based diagnostics that enables timely patient treatment and management.
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