{"title":"3d打印超可拉伸丝素基生物相容性水凝胶","authors":"Sushma Indrakumar , Alaka T. Panicker , Sampath Parasuram , Akshat Joshi , Tapan Kumar Dash , Vivek Mishra , Bharat Tandon , Kaushik Chatterjee","doi":"10.1016/j.bprint.2023.e00315","DOIUrl":null,"url":null,"abstract":"<div><p><span><span><span>Flexible hydrogels are extensively being explored for potential applications in biomedical devices<span><span> and flexible electronics. Long-term stability and excellent flexibility are two critical criteria for hydrogel-based devices. In this study, a ternary blend ink was formulated specifically for three-dimensional (3D) printing of stretchable hydrogels comprising silk fibroin, </span>polyvinyl alcohol, and </span></span>methylcellulose<span>. The ink composition was tuned to ensure favorable rheological properties for 3D printing. The printed hydrogels were subjected to methanol treatment to achieve the desired flexibility. The developed silk hydrogels exhibited superior </span></span>mechanical properties<span><span>: elongation at break (459 ± 5 %), breaking </span>strength<span> (137 ± 6 kPa), elastic modulus (37 ± 3 kPa), toughness (334 ± 7 kJ/m</span></span></span><sup>3</sup><span>), and hysteresis (1.1 ± 0.4 kJ/m</span><sup>2</sup>). Additionally, the hydrogel exhibited anti-fatigue and shape recovery abilities. The <em>in vitro</em> degradation study demonstrated the long-term stability of the hydrogel. Furthermore, the <em>in vivo</em><span><span> biocompatibility was evaluated by subcutaneous implantation of the printed construct in a rodent model. The </span>histological analysis<span> of the tissue morphology and assessment of blood parameters showed no hallmarks of adverse immune reaction or toxicity caused by the implanted construct. Overall, the developed silk-based ternary blend ink can serve as a potential material platform for 3D printing hydrogel-based implantable devices.</span></span></p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"3D-printed ultra-stretchable silk fibroin-based biocompatible hydrogels\",\"authors\":\"Sushma Indrakumar , Alaka T. Panicker , Sampath Parasuram , Akshat Joshi , Tapan Kumar Dash , Vivek Mishra , Bharat Tandon , Kaushik Chatterjee\",\"doi\":\"10.1016/j.bprint.2023.e00315\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span><span><span>Flexible hydrogels are extensively being explored for potential applications in biomedical devices<span><span> and flexible electronics. Long-term stability and excellent flexibility are two critical criteria for hydrogel-based devices. In this study, a ternary blend ink was formulated specifically for three-dimensional (3D) printing of stretchable hydrogels comprising silk fibroin, </span>polyvinyl alcohol, and </span></span>methylcellulose<span>. The ink composition was tuned to ensure favorable rheological properties for 3D printing. The printed hydrogels were subjected to methanol treatment to achieve the desired flexibility. The developed silk hydrogels exhibited superior </span></span>mechanical properties<span><span>: elongation at break (459 ± 5 %), breaking </span>strength<span> (137 ± 6 kPa), elastic modulus (37 ± 3 kPa), toughness (334 ± 7 kJ/m</span></span></span><sup>3</sup><span>), and hysteresis (1.1 ± 0.4 kJ/m</span><sup>2</sup>). Additionally, the hydrogel exhibited anti-fatigue and shape recovery abilities. The <em>in vitro</em> degradation study demonstrated the long-term stability of the hydrogel. Furthermore, the <em>in vivo</em><span><span> biocompatibility was evaluated by subcutaneous implantation of the printed construct in a rodent model. The </span>histological analysis<span> of the tissue morphology and assessment of blood parameters showed no hallmarks of adverse immune reaction or toxicity caused by the implanted construct. Overall, the developed silk-based ternary blend ink can serve as a potential material platform for 3D printing hydrogel-based implantable devices.</span></span></p></div>\",\"PeriodicalId\":37770,\"journal\":{\"name\":\"Bioprinting\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-10-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioprinting\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2405886623000581\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Computer Science\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioprinting","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405886623000581","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Computer Science","Score":null,"Total":0}
Flexible hydrogels are extensively being explored for potential applications in biomedical devices and flexible electronics. Long-term stability and excellent flexibility are two critical criteria for hydrogel-based devices. In this study, a ternary blend ink was formulated specifically for three-dimensional (3D) printing of stretchable hydrogels comprising silk fibroin, polyvinyl alcohol, and methylcellulose. The ink composition was tuned to ensure favorable rheological properties for 3D printing. The printed hydrogels were subjected to methanol treatment to achieve the desired flexibility. The developed silk hydrogels exhibited superior mechanical properties: elongation at break (459 ± 5 %), breaking strength (137 ± 6 kPa), elastic modulus (37 ± 3 kPa), toughness (334 ± 7 kJ/m3), and hysteresis (1.1 ± 0.4 kJ/m2). Additionally, the hydrogel exhibited anti-fatigue and shape recovery abilities. The in vitro degradation study demonstrated the long-term stability of the hydrogel. Furthermore, the in vivo biocompatibility was evaluated by subcutaneous implantation of the printed construct in a rodent model. The histological analysis of the tissue morphology and assessment of blood parameters showed no hallmarks of adverse immune reaction or toxicity caused by the implanted construct. Overall, the developed silk-based ternary blend ink can serve as a potential material platform for 3D printing hydrogel-based implantable devices.
期刊介绍:
Bioprinting is a broad-spectrum, multidisciplinary journal that covers all aspects of 3D fabrication technology involving biological tissues, organs and cells for medical and biotechnology applications. Topics covered include nanomaterials, biomaterials, scaffolds, 3D printing technology, imaging and CAD/CAM software and hardware, post-printing bioreactor maturation, cell and biological factor patterning, biofabrication, tissue engineering and other applications of 3D bioprinting technology. Bioprinting publishes research reports describing novel results with high clinical significance in all areas of 3D bioprinting research. Bioprinting issues contain a wide variety of review and analysis articles covering topics relevant to 3D bioprinting ranging from basic biological, material and technical advances to pre-clinical and clinical applications of 3D bioprinting.