津巴布韦传统医学中使用的部分康柏属植物提取物的抗霉菌、抗生物膜和外排泵抑制活性

IF 1.8 Q3 PHARMACOLOGY & PHARMACY
Sharon Netsai Chikafa, Chi Godloves Fru, Stanley Mukanganyama
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引用次数: 0

摘要

非结核分枝杆菌主要感染免疫力低下的人群。非结核分枝杆菌感染发病率和流行率的上升已成为一个公共卫生问题。Combretum 物种被人类和动物用作食物和药用。这项研究的目的是评估从选定的康普瑞特木物种中提取的植物提取物对烟分枝杆菌和金黄色葡萄球菌的抗霉菌作用,以及它们对最易感生物的作用模式。对植物提取物的抗霉菌活性进行了筛选。分枝杆菌对这些提取物更易感。研究了强效康柏提取物对罗丹明 6G 运输和烟曲霉生物膜的影响。此外,还研究了萃取物在烟曲霉体内积累的罗丹明 6G 量。C. zeyheri丙酮提取物是最有效的外排泵抑制剂。C. zeyheri 的表面提取物是最有效的生物膜破坏剂和抑制剂。另一个物种 C. apiculatum 的乙酸乙酯提取物对生物膜没有影响,这可能是由于提取物无法渗透到已经形成的生物膜中。对 C. zeyheri 的丙酮提取物进行的超高效液相色谱 UPHLC-MS/MS 分析表明,该提取物中含有卷曲霉素、奎宁酸和安瓿葡糖苷,这些物质可能有助于提高其抗炎活性。总之,津巴布韦传统医学中使用的 C. zeyheri、C. platypetalum 和 C. apicultum 物种的植物化学物质显示出抗结核菌和抗生物膜活性,可用作针对由烟曲霉菌引起的非结核感染的先导化合物来源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Antimycobacterial, antibiofilm and efflux pump inhibitory activity of extracts from selected Combretum species used in traditional medicine in Zimbabwe

Antimycobacterial, antibiofilm and efflux pump inhibitory activity of extracts from selected Combretum species used in traditional medicine in Zimbabwe

Non-tuberculous mycobacteria infect mostly immunocompromised people. NTM infections incidence and prevalence increase has led to a public health problem. Combretum species are used as food by humans and animals, and medicinal purposes. The aim of the study was to evaluate the antimycobacterial effects of plant extracts from selected Combretum species on Mycobacterium smegmatis and Mycobacterium aurum as well as their mode of action on the most susceptible organisms. Antimycobacterial activity of the plant extracts was screened. Mycobacterium smegmatis was more susceptible to the extracts. The potent Combretum extracts were investigated for their effects on Rhodamine 6G transport and biofilms in M. smegmatis. The amount of Rhodamine 6G accumulated in M. smegmatis in the presence of extracts were also investigated. The C. zeyheri acetone extracts were the most potent efflux pump inhibitors. Surface extracts from C. zeyheri were the most potent disrupters and inhibitors of biofilms. The ethylacetate extract of another species, C. apiculatum lacked the effect on biofilms and this may be due to the lack of penetration of the extract into the already established biofilm. The ultra-high performance liquid chromatography UPHLC-MS/MS analysis of acetone extracts from C. zeyheri indicated the presence of cirsimaritin, quinic acid, and ampelopsin glucoside and these could have contributed to the antimycobacterial activities of the extract. In conclusion, phytochemicals from C. zeyheri, C. platypetalum, C. apicultum species used in traditional medicine in Zimbabwe showed antimycobacterial and antibiofilm activity and may be used as sources of lead compounds targeting non-tuberculosis infections caused by M. smegmatis.

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来源期刊
Advances in Traditional Medicine
Advances in Traditional Medicine PHARMACOLOGY & PHARMACY-
CiteScore
4.30
自引率
0.00%
发文量
50
期刊介绍: Advances in Traditional Medicine (ADTM) is an international and peer-reviewed journal and publishes a variety of articles including original researches, reviews, short communications, and case-reports. ADTM aims to bridging the gap between Traditional knowledge and medical advances. The journal focuses on publishing valid, relevant, and rigorous experimental research and clinical applications of Traditidnal Medicine as well as medical classics. At the same time, the journal is devoted to communication among basic researcher and medical clinician interested in the advancement of Traditional Medicine. Topics covered by the journal are: Medical Classics & History; Biomedical Research; Pharmacology & Toxicology of Natural Products; Acupuncture & Moxibustion; Sasang Constitutional Medicine; Diagnostics and Instrumental Development; Clinical Research. ADTM is published four times yearly. The publication date of this journal is 30th March, June, September, and December.
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