Iraqi Hyacinthus orientalis L. Flowers as the Source of Bioactive Compounds Especially Stigmasterol: Identification, Isolation and Characterization

This research concentrated on the isolation and identification of stigmasterol from cultivated Iraqi Hyacinthus orientalis flowers part, as there are few studies that deal with these plant-specific flowers as e-research shows. Extraction were done for the flowers part of plant, by the soxhlet apparatus using 85% ethanol, followed by fractionation with petroleum ether to obtain the target of this study. Phytochemical screening was done by gas chromatography-mass spectrometry (GC-MS) for petroleum ether fraction qualitative and quantitative estimation and isolation of stigmasterol by reverse phase high-performance liquid chromatography (RP-HPLC), identification of isolated stigmasterol by Fourier transforms infrared (FTIR) spectroscopy, ultraviolet spectroscopy and liquid chromatography-mass spectroscopy (LC-MS). Announcing that the study considered the first to isolate stigmasterol from flowers part of Iraqi H. orientalis.