{"title":"通过外显子组捕获和重测序在豌豆遗传资源收集中发现SNP","authors":"Grégoire Aubert, Jonathan Kreplak, Magalie Leveugle, Hervé Duborjal, Anthony Klein, Karen Boucherot, Emilie Vieille, Marianne Chabert-Martinello, Corinne Cruaud, Virginie Bourion, Isabelle Lejeune-Hénaut, Marie-Laure Pilet-Nayel, Yanis Bouchenak-Khelladi, Nicolas Francillonne, Nadim Tayeh, Jean-Philippe Pichon, Nathalie Rivière, Judith Burstin","doi":"10.24072/pcjournal.332","DOIUrl":null,"url":null,"abstract":"Pea is a major pulse crop in temperate regions and a model plant in genetics. Large genetic marker resources are needed to assess the genetic diversity in the species genepool and to provide selection tools for breeders. In this study, we used second-generation sequencing to perform an exome-capture protocol using a diverse pea germplasm collection, and produced a resource of over 2 million Single Nucleotide Polymorphisms. This dataset was then used to characterize the genetic diversity present in the panel and compute phylogenetic and structure analyses. The development of this resource paves the way for Genome-wide association studies and the development of powerful genotyping tools.","PeriodicalId":74413,"journal":{"name":"Peer community journal","volume":"57 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"SNP discovery by exome capture and resequencing in a pea genetic resource collection\",\"authors\":\"Grégoire Aubert, Jonathan Kreplak, Magalie Leveugle, Hervé Duborjal, Anthony Klein, Karen Boucherot, Emilie Vieille, Marianne Chabert-Martinello, Corinne Cruaud, Virginie Bourion, Isabelle Lejeune-Hénaut, Marie-Laure Pilet-Nayel, Yanis Bouchenak-Khelladi, Nicolas Francillonne, Nadim Tayeh, Jean-Philippe Pichon, Nathalie Rivière, Judith Burstin\",\"doi\":\"10.24072/pcjournal.332\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Pea is a major pulse crop in temperate regions and a model plant in genetics. Large genetic marker resources are needed to assess the genetic diversity in the species genepool and to provide selection tools for breeders. In this study, we used second-generation sequencing to perform an exome-capture protocol using a diverse pea germplasm collection, and produced a resource of over 2 million Single Nucleotide Polymorphisms. This dataset was then used to characterize the genetic diversity present in the panel and compute phylogenetic and structure analyses. The development of this resource paves the way for Genome-wide association studies and the development of powerful genotyping tools.\",\"PeriodicalId\":74413,\"journal\":{\"name\":\"Peer community journal\",\"volume\":\"57 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-10-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Peer community journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.24072/pcjournal.332\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Peer community journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.24072/pcjournal.332","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
SNP discovery by exome capture and resequencing in a pea genetic resource collection
Pea is a major pulse crop in temperate regions and a model plant in genetics. Large genetic marker resources are needed to assess the genetic diversity in the species genepool and to provide selection tools for breeders. In this study, we used second-generation sequencing to perform an exome-capture protocol using a diverse pea germplasm collection, and produced a resource of over 2 million Single Nucleotide Polymorphisms. This dataset was then used to characterize the genetic diversity present in the panel and compute phylogenetic and structure analyses. The development of this resource paves the way for Genome-wide association studies and the development of powerful genotyping tools.