Nail Tekin ÖNDER, Taygun GÖKDEMİR, Muhammet Can KILIÇ, Savaş YILDIZ, Yavuz ÖZTÜRKLER
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Effects of Insulin Lispro on Ram Semen Cryopreservation
In the present study, it was aimed to evaluate the effects of semen extender with added insulin on ram semen at the post-thaw stage. Semen was collected from 5 Tuj rams five times with an electro-ejaculator. Experimental groups were designated as control group and groups containing 15 IU and 20 IU insulin lispro added semen extenders. At the post-thaw stage, motility and plasma membrane integrity were evaluated by microscopy. Acrosome integrity (Fitc-Peanut Agglutinin) and mitochondrial membrane potential (Rhodamine 123) were assessed by dual staining with propidium iodide (PI) using flow cytometry. It was found that motility and plasma membrane functional integrity were better preserved in the control group (p0.05), but acrosome integrity was negatively affected in the 20 IU insulin group (p
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