AL - Najaf AL - Ashraf /伊拉克严重腹泻儿童中产肠毒素大肠杆菌和肠侵袭性大肠杆菌的分布

Q4 Medicine
Malak Kahtan Jamal, Ahlam Kadhum Naeem, Hawraa I. Kadhim
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引用次数: 0

摘要

腹泻是导致5岁以下儿童死亡的第二大疾病。本研究旨在调查5岁以下儿童中与腹泻相关的肠侵入性大肠杆菌(EIEC)和产肠毒素大肠杆菌(ETEC)的流行情况。从患有急性腹泻的5岁以下儿童中收集了100个粪便样本。所有样品均在麦康基琼脂和伊红亚甲基蓝(EMB)培养基上培养,检测致泻性大肠杆菌(DEE)。进行DNA提取和PCR技术。用于检测ETEC的stp基因和检测EIEC的ipaH基因的扩增。结果显示,DEC在男性和女性中分布广泛(45%),其中男性感染比例高(60%),女性感染比例高(40%)。琼脂糖凝胶电泳结果显示,33%的分离株具有ipaH,表明这些分离株属于EIEC。相比之下,22%的分离株具有stp基因,表明这些分离株属于肠出血性大肠杆菌。总之,腹泻可能与EIEC和EHEC有关,除了EPEC。关键词:产肠毒素大肠杆菌,肠侵袭性大肠杆菌,重症腹泻患儿,聚合酶链反应PCR
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Distribution of Enterotoxigenic E. coli and Enteroinvasive E. Coli Among Children with Severe Diarrhea in AL Najaf AL Ashraf / Iraq
Diarrhea is the second most important disease that causes death in children under 5 years old. This research aimed to investigate the prevalence of Enteroinvasive E. coli (EIEC) and Enterotoxigenic E. coli (ETEC) associated with diarrhea among children under 5 years old. One hundred stool samples have been collected from children under 5 years old suffering from acute diarrhea. All samples are cultured on MacConkey agar and Eosin Methylene Blue (EMB) to detect Diarrheagenic E. coli (DEE). DNA extraction was carried out, and PCR technique. It was used for amplification of the stp gene for the detection of ETEC and the ipaH gene for the detection of EIEC. The results showed a wide distribution of DEC (45%) among males and females, where a high percentage of infection occurred among males (60%) in Compression with females (40%). The Agarose gel electrophoresis results showed that 33% of isolates possessed ipaH, which revealed that these isolates belonged to EIEC. In comparison, 22% of isolates possessed the stp gene, which revealed that these isolates were belong to EHEC. In conclusion, diarrhea may be associated with EIEC and EHEC in addition to EPEC. Keywords: Enterotoxigenic E. coli, Enteroinvasive E. coli, Children with Severe Diarrhea, polymerase chain reaction PCR
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来源期刊
Revista Bionatura
Revista Bionatura Medicine-Epidemiology
CiteScore
0.50
自引率
0.00%
发文量
144
审稿时长
8 weeks
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