Dwi Apriliani, Lia Handayani, Nadia Putri, Reza Zuhrayani, Faisal Syahputra
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引用次数: 0
摘要
皮桑虾壳中含有大量的甲壳素,是最能作为壳聚糖利用的渔业废弃物之一。此外,毗桑虾也是亚齐地区特有的虾种,因此其作为壳聚糖使用的研究资料仍然非常有限。采用两级法分离甲壳素化合物。阶段为脱矿和脱蛋白。第一次用1 N HCl溶液,比(1:12)w/v脱矿120小时,然后用1 N HCl (1:5) w/v第二次脱矿反应20小时。脱蛋白反应采用3n NaOH溶液(1:10)w/v, 90℃,50 rpm, 1小时。用50%(1:10)的氢氧化钠(NaOH),在120℃下,1000 rpm,反应10小时,将甲壳素转化为壳聚糖。以皮桑虾壳为原料,用100目筛将其磨成均匀大小的筛粉。皮桑虾壳粉有特点,含水量0.75%;灰分32.48%;脂肪2.12%,总氮34.13%。壳聚糖质量分数为灰分含量0.72%;n -总量为2.73%,脱乙酰度为76%,壳聚糖收率为23.6%。以虾壳粉的用量为基准,计算壳聚糖的收率
Isolasi kitosan dari cangkang udang pisang (Penaeus sp) sebagai spesies endemik di pantai barat Aceh
Pisang shrimp shells are one of the most fisheries waste can be utilized as chitosan because it contains lots of chitin. In addition, Pisang shrimp is also an endemic shrimp species in Aceh, so research data related to its use as chitosan is still very limited. Isolation chitin compound by using 2 stages. The stages are demineralization and deproteination. The demineralization with 1 N HCl solution, ratio (1:12) w/v for 120 hours, and then the second demineralization reaction with 1 N HCl (1:5) w/v for 20 hours. deproteinization reaction used 3 N NaOH solution (1:10) w/v, at 90 ºC for 1 hour with 50 rpm. Reaction transformation chitin into chitosan using NaOH 50 % (1:10) w/v, at 120ºC, for 10 hours with 1000 rpm. Pisang shrimp shells that used as raw material were milled to uniform size of the sifted flour using a sieve of 100 mesh sieve. Pisang shrimp shells flour has characterized, water content 0.75 %; ash content 32.48 %; fat 2.12 % and N-total 34,13 %. The quality of chitosan were ash content 0.72 %; N-total 2.73 %, the degree of deacetylation 76 % and the yield of chitosan obtained was 23.6 %. the yield was calculated based on the chitosan produced from the amount of pisang shrimp shell flour used