在冷冻剂中添加褪黑素可提高犬附睾精子质量

IF 2.1 4区 医学 Q3 ANDROLOGY
Andrologia Pub Date : 2023-10-03 DOI:10.1155/2023/7873399
Eman Fayez, Haney Samir, Mohamed A. I. El Sayed, Zaher M. Rawash, Ali Salama
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引用次数: 0

摘要

本研究旨在探讨褪黑素对犬附睾精子质量的冷冻保护作用。将20只去势犬的附睾切碎,置于Tris缓冲液中孵育。精子在三柠檬酸-果糖(TCF)扩展剂中稀释,加入两种剂量的褪黑激素(0.002和0.0035 mol)以及对照(0.0 mol)。在5°C下平衡2小时后,将样品装入0.25 ml吸管中,并保存在液氮(- 196°C)中。在解冻(37°C 30 s)后评估精子的活力、活力、顶体完整性、精子膜功能和DNA完整性等参数。此外,通过测定丙二醛(MDA)水平来评估脂质过氧化。结果表明,在冷冻保存培养基中添加0.0035 mol褪黑素可增强(P <;与对照组和0.002 mol褪黑素相比,精子的测量参数降低了0.05)。丙二醛水平明显升高(P <;与0.002 mol褪黑素和对照组相比,添加0.0035 mol褪黑素时降低了0.05)。最终,在TCF扩展剂中添加0.0035 mol褪黑素显著提高了犬附睾精子的活力、活力、质膜完整性和顶体完整性。此外,褪黑素的补充改善了DNA的完整性,减少了膜脂过氧化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Melatonin Supplementation to the Freezing Extender Improves the Quality of Canine Epididymal Spermatozoa

Melatonin Supplementation to the Freezing Extender Improves the Quality of Canine Epididymal Spermatozoa

The present study aimed to determine the cryoprotective effect of melatonin supplements on the extender on the quality of canine epididymal spermatozoa. Epididymis from 20 castrated dogs were minced and incubated in Tris buffer. Spermatozoa were diluted in tris-citric acid-fructose (TCF) extender with two doses of melatonin (0.002 and 0.0035 mol) as well as a control (0.0 mol). The samples were packed in 0.25 ml straws after 2 hr of equilibration at 5°C and kept in liquid nitrogen (−196°C). Sperm parameters such as viability, motility, integrity of acrosome, sperm membrane functioning, and DNA integrity were assessed after thawing (37°C for 30 s). In addition, lipid peroxidation was assessed using the determination of malondialdehyde (MDA) levels. Results revealed that adding 0.0035 mol melatonin to the cryopreservation medium enhanced (P < 0.05) the measured parameters of spermatozoa compared to the control and 0.002 mol melatonin. Levels of MDA were obviously (P < 0.05) lower when 0.0035 mol melatonin was added in comparison to 0.002 mol melatonin and the control. Eventually, adding 0.0035 mol melatonin to the TCF extender significantly improved the percentage of motility, viability, and integrity of both plasma membrane and acrosome integrity of dog epididymal spermatozoa. In addition, melatonin supplementation improved DNA integrity and reduced membrane lipid peroxidation.

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来源期刊
Andrologia
Andrologia 医学-男科学
CiteScore
5.60
自引率
8.30%
发文量
292
审稿时长
6 months
期刊介绍: Andrologia provides an international forum for original papers on the current clinical, morphological, biochemical, and experimental status of organic male infertility and sexual disorders in men. The articles inform on the whole process of advances in andrology (including the aging male), from fundamental research to therapeutic developments worldwide. First published in 1969 and the first international journal of andrology, it is a well established journal in this expanding area of reproductive medicine.
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