hamiltonii Wight的微繁及遗传保真度评价采用RAPD标记

Q4 Biochemistry, Genetics and Molecular Biology

Biochemical and Cellular Archives Pub Date : 2023-09-01 DOI:10.51470/bca.2023.23.2.721

M. Saminathan, A. Muruganandam, M. Arumugam, Amzad Basha Kolar

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引用次数: 0

摘要

微繁植物的体细胞无性系变异改善了作物。遗传转化研究依赖于植物微繁遗传的完整性。体细胞无性系变异在植物微繁繁殖中经常发生，引起了对植物遗传稳定性的研究。在添加了0.5 mg L-1苄氨基嘌呤(BAP)的Murashige和Skoog (MS)培养基中，以反向接种的方式接种黄芪茎尖外植体，可获得较高的芽数。在添加0.5 mg L-1苄氨基嘌呤(BAP)和0.1 mg L-1吲哚-3-乙酸(IAA)的MS培养基上，芽长增加。在添加了1 mg L-1吲哚-3-丁酸(IBA)的Murashige和Skoog (MS)培养基中培养增长芽。扎根良好的植物经历了温室硬化。微繁殖和种子繁殖的植物具有相似的物理特征。采用随机扩增多态性DNA (RAPD)方法，利用10条引物对再生植株的遗传稳定性进行了评价。鉴定出85个波段。在田间种植的植株和条带中发现了单态特征和原始型无性系。再生方法可能有助于研究哈密顿菌的微繁殖

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Micropropagation and assessment of genetic fidelity of Decalepis hamiltonii Wight & Arn using RAPD markers

Somaclonal variation in micropropagated plants improves crops. Genetic transformation research relies on micropropagated plant genetic integrity. Somaclonal variation frequently occurs in micropropagation, prompting an investigation of plant genetic stability. The high number of shoot buds was seen when Decalepis hamiltonii shooting tip explants were inoculated with reversed polarity in Murashige and Skoog (MS) medium supplemented with 0.5 mg L-1 benzylaminopurine (BAP). The shoot buds increased in length on an MS medium supplemented with 0.5 mg L-1 Benzylaminopurine (BAP) and 0.1 mg L-1 indole-3-acetic acid (IAA). The lengthened shoots were established in Murashige and Skoog (MS) medium supplemented with 1 mg L-1 Indole-3-butyric Acid (IBA). Well-rooted plants underwent greenhouse hardening. Micropropagated and seedpropagated plants have similar physical features. Regenerated plant genetic stability was assessed using ten primers in a Random Amplified Polymorphic DNA (RAPD) assay. Eighty-five bands were identified. Monomorphic features and original-type clones were found in field-grown plants and bands. The regeneration approach might help study D. hamiltonii micropropagation

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来源期刊

Biochemical and Cellular Archives Biochemistry, Genetics and Molecular Biology-Biochemistry

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期刊介绍： Biochemical and Cellular Archives – publishes original full length full length research papers, short communications and review articles in all areas of agricultural, biological and medical sciences. Emphasis will be given to manuscript which present novel finding pertinent to the biochemical basis of cellular structure and function as well as mechanism of cellular organelles. Investigations directed towards electron microscopy and localization of biomolecules in cells / tissues are particularly encouraged. The articles for the following broad areas of biological sciences will be considered for publications: ● Cellular Structure, Physiology and Biochemistry. ● Bacteriology, Virology and Toxicology. ● Light and electron microscopy and histochemistry. ● Immunology and molecular biology. ● Pathology.