Nadia Denys Betancourt-Martínez, Pilar Carranza-Rosales, Ezequiel Viveros-Valdez, Nancy Elena Guzmán-Delgado, Maritza Argelia Macias-Corral, Irma Edith Carranza-Torres, Lydia Enith Nava-Rivera, María Soñadora Niño Castañeda, Javier Morán-Martínez
{"title":"drummondii肿对甲醛致Wistar大鼠睾丸毒性和遗传毒性的保护作用","authors":"Nadia Denys Betancourt-Martínez, Pilar Carranza-Rosales, Ezequiel Viveros-Valdez, Nancy Elena Guzmán-Delgado, Maritza Argelia Macias-Corral, Irma Edith Carranza-Torres, Lydia Enith Nava-Rivera, María Soñadora Niño Castañeda, Javier Morán-Martínez","doi":"10.1155/2023/6631345","DOIUrl":null,"url":null,"abstract":"<div>\n <p>Formaldehyde (FA) is an oxidative stress inducer and a known carcinogen. <i>Hedeoma drummondii</i> has been shown to have potential as a chemopreventive agent. The aim of this study was to assess the protective effect of <i>H. drummondii</i> on FA-induced genotoxic damage, sperm quality parameters, and histological changes of the testis. The male Wistar rats used were divided into the following groups: (G1) 100 mg/kg <i>H. drummondii</i>; (G2) 10 mg/kg FA; (G3) 100 mg/kg <i>H. drummondii</i> + 10 mg/kg FA; (G4) phosphate-buffered saline; (G5) 100 mg/kg <i>α</i>-tocopherol; (G6) 100 mg/kg <i>α</i>-tocopherol + 10 mg/kg FA; and (G7) soybean oil. The results showed a sperm concentration of 23.66 ± 1.52 versus 33.67 cells/mL (<i>p</i> = 0.02), and a percentage of motility of 29.66 ± 1.51 versus 33.33 (<i>p</i> = 0.620), for groups G3 and G6, respectively. Histopathological (H&E) and immunohistochemical analysis of superoxide dismutase showed damage in the germinal epithelium (GE) in G2. While in groups G3 and G6, the histological structure was preserved. A protective effect on sperm DNA fragmentation was also observed for treated groups (G3 and G6, <i>p</i> < 0.0001 vs. G2). While in groups G3 and G6 the histological structure was preserved. A protective effect on sperm DNA fragmentation was also observed for treated groups (G3 and G6). In conclusion, FA exposure significantly reduces sperm quality parameters, causes sperm DNA damage, and alters GE in the testis, while <i>H. drummondii</i> has a protective effect against FA-induced sperm genotoxic damage, similar to <i>α</i>-tocopherol.</p>\n </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2023 1","pages":""},"PeriodicalIF":2.1000,"publicationDate":"2023-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2023/6631345","citationCount":"0","resultStr":"{\"title\":\"Protective Effect of Hedeoma drummondii against Formaldehyde-Induced Testicular Toxicity and Genotoxicity in Wistar Rats\",\"authors\":\"Nadia Denys Betancourt-Martínez, Pilar Carranza-Rosales, Ezequiel Viveros-Valdez, Nancy Elena Guzmán-Delgado, Maritza Argelia Macias-Corral, Irma Edith Carranza-Torres, Lydia Enith Nava-Rivera, María Soñadora Niño Castañeda, Javier Morán-Martínez\",\"doi\":\"10.1155/2023/6631345\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n <p>Formaldehyde (FA) is an oxidative stress inducer and a known carcinogen. <i>Hedeoma drummondii</i> has been shown to have potential as a chemopreventive agent. The aim of this study was to assess the protective effect of <i>H. drummondii</i> on FA-induced genotoxic damage, sperm quality parameters, and histological changes of the testis. The male Wistar rats used were divided into the following groups: (G1) 100 mg/kg <i>H. drummondii</i>; (G2) 10 mg/kg FA; (G3) 100 mg/kg <i>H. drummondii</i> + 10 mg/kg FA; (G4) phosphate-buffered saline; (G5) 100 mg/kg <i>α</i>-tocopherol; (G6) 100 mg/kg <i>α</i>-tocopherol + 10 mg/kg FA; and (G7) soybean oil. The results showed a sperm concentration of 23.66 ± 1.52 versus 33.67 cells/mL (<i>p</i> = 0.02), and a percentage of motility of 29.66 ± 1.51 versus 33.33 (<i>p</i> = 0.620), for groups G3 and G6, respectively. Histopathological (H&E) and immunohistochemical analysis of superoxide dismutase showed damage in the germinal epithelium (GE) in G2. While in groups G3 and G6, the histological structure was preserved. A protective effect on sperm DNA fragmentation was also observed for treated groups (G3 and G6, <i>p</i> < 0.0001 vs. G2). While in groups G3 and G6 the histological structure was preserved. A protective effect on sperm DNA fragmentation was also observed for treated groups (G3 and G6). In conclusion, FA exposure significantly reduces sperm quality parameters, causes sperm DNA damage, and alters GE in the testis, while <i>H. drummondii</i> has a protective effect against FA-induced sperm genotoxic damage, similar to <i>α</i>-tocopherol.</p>\\n </div>\",\"PeriodicalId\":7817,\"journal\":{\"name\":\"Andrologia\",\"volume\":\"2023 1\",\"pages\":\"\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2023-10-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2023/6631345\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Andrologia\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1155/2023/6631345\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ANDROLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Andrologia","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1155/2023/6631345","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ANDROLOGY","Score":null,"Total":0}
Protective Effect of Hedeoma drummondii against Formaldehyde-Induced Testicular Toxicity and Genotoxicity in Wistar Rats
Formaldehyde (FA) is an oxidative stress inducer and a known carcinogen. Hedeoma drummondii has been shown to have potential as a chemopreventive agent. The aim of this study was to assess the protective effect of H. drummondii on FA-induced genotoxic damage, sperm quality parameters, and histological changes of the testis. The male Wistar rats used were divided into the following groups: (G1) 100 mg/kg H. drummondii; (G2) 10 mg/kg FA; (G3) 100 mg/kg H. drummondii + 10 mg/kg FA; (G4) phosphate-buffered saline; (G5) 100 mg/kg α-tocopherol; (G6) 100 mg/kg α-tocopherol + 10 mg/kg FA; and (G7) soybean oil. The results showed a sperm concentration of 23.66 ± 1.52 versus 33.67 cells/mL (p = 0.02), and a percentage of motility of 29.66 ± 1.51 versus 33.33 (p = 0.620), for groups G3 and G6, respectively. Histopathological (H&E) and immunohistochemical analysis of superoxide dismutase showed damage in the germinal epithelium (GE) in G2. While in groups G3 and G6, the histological structure was preserved. A protective effect on sperm DNA fragmentation was also observed for treated groups (G3 and G6, p < 0.0001 vs. G2). While in groups G3 and G6 the histological structure was preserved. A protective effect on sperm DNA fragmentation was also observed for treated groups (G3 and G6). In conclusion, FA exposure significantly reduces sperm quality parameters, causes sperm DNA damage, and alters GE in the testis, while H. drummondii has a protective effect against FA-induced sperm genotoxic damage, similar to α-tocopherol.
期刊介绍:
Andrologia provides an international forum for original papers on the current clinical, morphological, biochemical, and experimental status of organic male infertility and sexual disorders in men. The articles inform on the whole process of advances in andrology (including the aging male), from fundamental research to therapeutic developments worldwide. First published in 1969 and the first international journal of andrology, it is a well established journal in this expanding area of reproductive medicine.