A. S. Ilchenko, B. F. Varenyk, N. P. Lamary, S. I. Karapira
{"title":"乌克兰南部草原湿度不足条件下向日葵对甲基三苯脲抗性的遗传","authors":"A. S. Ilchenko, B. F. Varenyk, N. P. Lamary, S. I. Karapira","doi":"10.15407/agrisp10.02.038","DOIUrl":null,"url":null,"abstract":"Aim. This study aimed to investigate the inheritance of resistance to sulfonylurea herbicides in sunflower under conditions of insufficient humidification in the southern Steppe of Ukraine. Methods. Isolation, castration, hybridization, herbicide application (Granstar Pro 75 % w.g., containing tribenuron methyl as the active substance (a.s.)), evaluation of herbicide resistance, and statistical analysis of the acquired data. Results. The investigation into the inheritance of resistance to sulfonylurea herbicides involved the utilization of both resistant (SURES-1, OS 1099 V, OS 2017 V) and non-resistant (Od 1002 B, Od 1318 V, OS 1295 V) sunflower genotypes. Through crossing, four F1 hybrid combinations were generated, namely OS 2017 V × OS 1099 V, SURES-1 × Od 1002 B, SURES-1 × Od 1318 V, and Od 1318 V x OS 1295 V. Subsequent treatment of F1 plants with the herbicide Granstar Pro 75 % w.g. revealed that three combinations (OS 2017 V × OS 1099 V, SURES-1 × Od 1002 B, SURES-1 × Od 1318 V) exhibited complete resistance to the herbicide. In the second generation, following herbicide treatment, the hybrid combinations SURES-1 × Od 1002 B and SURES-1 × Od 1318 V displayed segregation into resistant and non-resistant plants. Conversely, the plants in the combination OS 2017 V × OS 1099 V maintained complete resistance to the herbicidal effects. Conclusions. The investigation, conducted in the challenging climatic conditions of the southern Steppe of Ukraine, demonstrated complete resistance to sulfonylurea herbicides in three hybrid combinations of both F1 and F2 generations. Notably, the combination OS 2017 V × OS 1099 V exhibited uniform resistance throughout the second generation, devoid of segregation. Moreover, the results of F2 segregation analysis in the SURES-1 × Od 1002 B and SURES-1 × Od 1318 V populations indicated that resistance to tribenuron methyl is primarily governed by the presence of a dominant gene allele. These findings offer valuable insights for the development of sunflower hybrids with enhanced herbicide resistance, particularly in regions with adverse climatic conditions.","PeriodicalId":55933,"journal":{"name":"Agricultural Science and Practice","volume":null,"pages":null},"PeriodicalIF":0.4000,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Inheriting the resistance of sunflower to tribenuron methyl under insufficient humidification in the southern Steppe of Ukraine\",\"authors\":\"A. S. Ilchenko, B. F. Varenyk, N. P. Lamary, S. I. Karapira\",\"doi\":\"10.15407/agrisp10.02.038\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Aim. This study aimed to investigate the inheritance of resistance to sulfonylurea herbicides in sunflower under conditions of insufficient humidification in the southern Steppe of Ukraine. Methods. Isolation, castration, hybridization, herbicide application (Granstar Pro 75 % w.g., containing tribenuron methyl as the active substance (a.s.)), evaluation of herbicide resistance, and statistical analysis of the acquired data. Results. The investigation into the inheritance of resistance to sulfonylurea herbicides involved the utilization of both resistant (SURES-1, OS 1099 V, OS 2017 V) and non-resistant (Od 1002 B, Od 1318 V, OS 1295 V) sunflower genotypes. Through crossing, four F1 hybrid combinations were generated, namely OS 2017 V × OS 1099 V, SURES-1 × Od 1002 B, SURES-1 × Od 1318 V, and Od 1318 V x OS 1295 V. Subsequent treatment of F1 plants with the herbicide Granstar Pro 75 % w.g. revealed that three combinations (OS 2017 V × OS 1099 V, SURES-1 × Od 1002 B, SURES-1 × Od 1318 V) exhibited complete resistance to the herbicide. In the second generation, following herbicide treatment, the hybrid combinations SURES-1 × Od 1002 B and SURES-1 × Od 1318 V displayed segregation into resistant and non-resistant plants. Conversely, the plants in the combination OS 2017 V × OS 1099 V maintained complete resistance to the herbicidal effects. Conclusions. The investigation, conducted in the challenging climatic conditions of the southern Steppe of Ukraine, demonstrated complete resistance to sulfonylurea herbicides in three hybrid combinations of both F1 and F2 generations. Notably, the combination OS 2017 V × OS 1099 V exhibited uniform resistance throughout the second generation, devoid of segregation. Moreover, the results of F2 segregation analysis in the SURES-1 × Od 1002 B and SURES-1 × Od 1318 V populations indicated that resistance to tribenuron methyl is primarily governed by the presence of a dominant gene allele. These findings offer valuable insights for the development of sunflower hybrids with enhanced herbicide resistance, particularly in regions with adverse climatic conditions.\",\"PeriodicalId\":55933,\"journal\":{\"name\":\"Agricultural Science and Practice\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.4000,\"publicationDate\":\"2023-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Agricultural Science and Practice\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15407/agrisp10.02.038\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Agricultural Science and Practice","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15407/agrisp10.02.038","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
摘要
的目标。本研究旨在研究乌克兰南部草原向日葵在湿度不足条件下对磺酰脲类除草剂的抗性遗传。方法。分离、去势、杂交、施用除草剂(Granstar Pro 75% w.g.g,含有甲基三苯脲作为活性物质(a.s))、除草剂抗性评估,并对获得的数据进行统计分析。结果。利用抗性向日葵基因型(SURES-1、OS 1099 V、OS 2017 V)和非抗性向日葵基因型(Od 1002 B、Od 1318 V、OS 1295 V)对磺酰脲类除草剂的抗性遗传进行了研究。通过杂交得到OS 2017 V × OS 1099 V、SURES-1 × Od 1002 B、SURES-1 × Od 1318 V、Od 1318 V × OS 1295 V四个F1杂交组合。随后用除草剂Granstar Pro 75% w.g.处理F1植株,发现3种组合(OS 2017 V × OS 1099 V、SURES-1 × Od 1002 B、SURES-1 × Od 1318 V)对该除草剂表现出完全抗性。在第二代除草剂处理后,SURES-1 × Od 1002b和SURES-1 × Od 1318v表现出抗性和非抗性植株的分离。相反,在OS 2017 V × OS 1099 V组合中,植株对除草效果保持完全抗性。结论。在乌克兰南部草原具有挑战性的气候条件下进行的调查表明,F1和F2代的三个杂交组合对磺酰脲类除草剂具有完全抗性。值得注意的是,OS 2017 V × OS 1099 V的组合在整个第二代中表现出均匀的电阻,没有隔离。此外,对SURES-1 × Od 1002 B和SURES-1 × Od 1318 V群体的F2分离分析结果表明,对甲基三苯脲的抗性主要由显性等位基因的存在决定。这些发现为开发抗除草剂能力增强的向日葵杂交品种提供了宝贵的见解,特别是在气候条件恶劣的地区。
Inheriting the resistance of sunflower to tribenuron methyl under insufficient humidification in the southern Steppe of Ukraine
Aim. This study aimed to investigate the inheritance of resistance to sulfonylurea herbicides in sunflower under conditions of insufficient humidification in the southern Steppe of Ukraine. Methods. Isolation, castration, hybridization, herbicide application (Granstar Pro 75 % w.g., containing tribenuron methyl as the active substance (a.s.)), evaluation of herbicide resistance, and statistical analysis of the acquired data. Results. The investigation into the inheritance of resistance to sulfonylurea herbicides involved the utilization of both resistant (SURES-1, OS 1099 V, OS 2017 V) and non-resistant (Od 1002 B, Od 1318 V, OS 1295 V) sunflower genotypes. Through crossing, four F1 hybrid combinations were generated, namely OS 2017 V × OS 1099 V, SURES-1 × Od 1002 B, SURES-1 × Od 1318 V, and Od 1318 V x OS 1295 V. Subsequent treatment of F1 plants with the herbicide Granstar Pro 75 % w.g. revealed that three combinations (OS 2017 V × OS 1099 V, SURES-1 × Od 1002 B, SURES-1 × Od 1318 V) exhibited complete resistance to the herbicide. In the second generation, following herbicide treatment, the hybrid combinations SURES-1 × Od 1002 B and SURES-1 × Od 1318 V displayed segregation into resistant and non-resistant plants. Conversely, the plants in the combination OS 2017 V × OS 1099 V maintained complete resistance to the herbicidal effects. Conclusions. The investigation, conducted in the challenging climatic conditions of the southern Steppe of Ukraine, demonstrated complete resistance to sulfonylurea herbicides in three hybrid combinations of both F1 and F2 generations. Notably, the combination OS 2017 V × OS 1099 V exhibited uniform resistance throughout the second generation, devoid of segregation. Moreover, the results of F2 segregation analysis in the SURES-1 × Od 1002 B and SURES-1 × Od 1318 V populations indicated that resistance to tribenuron methyl is primarily governed by the presence of a dominant gene allele. These findings offer valuable insights for the development of sunflower hybrids with enhanced herbicide resistance, particularly in regions with adverse climatic conditions.