逆转录酶治疗胶质母细胞瘤细胞的端粒动力学、基因表达和遗传不稳定性

Fábio Morato de Oliveira, Aline Monezi Montel, Wagner Gouvêa dos Santos
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引用次数: 0

摘要

背景:在本研究中,我们分析了逆转素(一种用于干细胞去分化和选择性诱导细胞死亡的小分子)对两种人类胶质母细胞瘤细胞系的细胞毒性作用。在暴露于不同浓度的逆转录酶后,在两种细胞系中量化AURKA和AURKB基因表达,并在3D尺度上分析逆转录酶对端粒动力学的影响。AURKA和AURKB基因表达有丝分裂激酶,在若干有丝分裂事件的调控中起重要作用。这些基因的高表达见于预后不良的细胞遗传学异常患者。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Telomere Dynamics, Gene Expression and Genetic Instability in Glioblastoma Cells Treated with Reversine
Background: In the present study, we analyzed the cytotoxic effect of reversine, a small molecule used for stem cell dedifferentiation and potential to selectively induce cell death, on two human glioblastoma cell lines. The AURKA and AURKB gene expression were quantified in both cell lines following the exposure to different concentrations of reversine, and the effect of reversine on telomere dynamics, in a 3D scale was analyzed. AURKA and AURKB genes express mitotic kinases with an important role in the regulation of several mitotic events. Hyperexpression of these genes are found in patients with cytogenetic abnormalities presenting an unfavorable prognosis.
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