含HIF-1α siRNA的金纳米复合材料对鼻咽癌细胞放疗的影响

IF 0.6 4区医学 Q4 PHARMACOLOGY & PHARMACY

Tropical Journal of Pharmaceutical Research Pub Date : 2023-11-06 DOI:10.4314/tjpr.v22i10.10

Haosheng Zhang, Fangzheng Zhou

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引用次数: 0

摘要

目的:研究携带缺氧诱导因子-1α (HIF-1α) siRNA的金纳米复合材料对鼻咽癌(NPC)细胞放射敏感性的影响。方法:首次合成金纳米复合物(AuNRs-(PEI-PEG)/RGD)或(AuPPR)，并对其细胞毒性进行评价。然后检测缺氧鼻咽癌细胞中HIF-1α的表达。此外，在x射线照射下检测携带HIF-1α siRNA的AuPPR对细胞的辐射敏感性。结果:携带HIF-1α siRNA的金纳米复合物(AuPPR) (AuPPR-HIF-1α siRNA)显著下调HIF-1α的表达。照射下，AuPPR- hif -1α siRNA显著促进鼻咽癌细胞凋亡，与对照组和单纯AuPPR组(22.5±4.16%，p <0.01)。此外，AuPPR-HIF-1α siRNA放疗组细胞周期明显阻滞在亚g1期，表明细胞损伤严重。结论:本研究表明，AuPPR-HIF-1α siRNA能有效改善鼻咽癌细胞在缺氧条件下的放射敏感性，因此有可能用于改善鼻咽癌的放疗

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Effect of gold nanocomposite bearing HIF-1α siRNA on radiotherapy in nasopharyngeal carcinoma cell

Purpose: To investigate the effect of gold nanocomposite carrying hypoxia-inducible factor-1α (HIF-1α) siRNA on radiosensitivity of nasopharynx cancer (NPC) cells. Methods: Gold nanocomposite (AuNRs-(PEI-PEG)/RGD) or (AuPPR) was first synthesized and its cytotoxicity was evaluated. Then, the expression of HIF-1α in hypoxic NPC cells was assessed. In addition, the radiation sensitivity of AuPPR bearing HIF-1α siRNA on the cells was examined under xray exposure. Result: Gold nanocomposite (AuPPR) carrying HIF-1α siRNA (AuPPR-HIF-1α siRNA) significantly down-regulated HIF-1α expression. Under irradiation treatment, AuPPR-HIF-1α siRNA significantly enhanced apoptosis of NPC cells by 33.76 ± 3.65 %, when compared to the control and simple AuPPR group (22.5 ± 4.16 %, p < 0.01). Furthermore, cell cycle was significantly blocked in the sub-G1 phase in AuPPR-HIF-1α siRNA radiotherapy groups, indicating severe cell damage. Conclusion: This study has demonstrated that AuPPR-HIF-1α siRNA is effective in improving the radiosensitivity of nasopharyngeal carcinoma cells under hypoxia, and therefore can potentially be used to improve the radiotherapy of this carcinoma

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来源期刊

Tropical Journal of Pharmaceutical Research 医学-药学

CiteScore

1.00

自引率

33.30%

发文量

490

审稿时长

4-8 weeks

期刊介绍： We seek to encourage pharmaceutical and allied research of tropical and international relevance and to foster multidisciplinary research and collaboration among scientists, the pharmaceutical industry and the healthcare professionals. We publish articles in pharmaceutical sciences and related disciplines (including biotechnology, cell and molecular biology, drug utilization including adverse drug events, medical and other life sciences, and related engineering fields). Although primarily devoted to original research papers, we welcome reviews on current topics of special interest and relevance.