Min-Yong Lee , Hi-Won Yoon , Kwang-Mahn Kim , Jae-Sung Kwon
{"title":"加入纳米银粒子和氟化钙的三氧化二矿骨料逆行填充材料的抗菌功效和成骨潜力","authors":"Min-Yong Lee , Hi-Won Yoon , Kwang-Mahn Kim , Jae-Sung Kwon","doi":"10.1016/j.jds.2023.10.001","DOIUrl":null,"url":null,"abstract":"<div><h3>Background/purpose</h3><p>The retrograde filling material, particularly mineral trioxide aggregate (MTA) employed in apicoectomy, should possess high antibacterial efficacy and osteogenic potential. We evaluated the antibacterial efficacy, biocompatibility, and osteogenic potential following the addition of silver nanoparticles (AgNPs) and calcium fluoride (CaF<sub>2</sub>) in retrograde filling material of MTA.</p></div><div><h3>Materials and methods</h3><p>MTA was mixed with four different solvents. Group 1 (G1): distilled water, Group 2 (G2): 50 ppm AgNPs, Group 3 (G3): 1 wt% CaF<sub>2</sub>, and Group 4 (G4): 50 ppm AgNPs and 1 wt% CaF<sub>2</sub>. The pH variation of each group was monitored, while the surface roughness was measured. The antibacterial efficacy against <em>Enterococcus faecalis (E. faecalis)</em> and the viability of murine pre-osteoblast (MC3T3) were evaluated for each group using colorimetric assays. The gene expression levels of osteogenic potential marker (OCN, ALPL, and RUNX2) in MC3T3 cells for each group were quantified using real-time-qPCR. Statistical analysis was performed at α = 0.05 level of significance.</p></div><div><h3>Results</h3><p>When comparing the levels of antibacterial efficacy, the order of effectiveness was G4>G2>G3>G1 (<em>P</em> < 0.05). In the cell viability test, owing to MTA-eluted growth medium having a positive effect on MC3T3 cell proliferation, G1–4 exhibited a statistically increased cell viability compared to the control (<em>P</em> < 0.05). However, G2–4 did not result in a statistically significant difference when compared to G1 (<em>P</em> < 0.05). Moreover, G4 exhibited the highest gene expression among the four groups (<em>P</em> < 0.05).</p></div><div><h3>Conclusion</h3><p>The addition of AgNPs and CaF<sub>2</sub> to MTA could be a promising option for use as a new retrograde filling material.</p></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"19 3","pages":"Pages 1783-1791"},"PeriodicalIF":3.4000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1991790223003367/pdfft?md5=ff4fa48d97abd875da151f98409b0f6a&pid=1-s2.0-S1991790223003367-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Antibacterial efficacy and osteogenic potential of mineral trioxide aggregate-based retrograde filling material incorporated with silver nanoparticle and calcium fluoride\",\"authors\":\"Min-Yong Lee , Hi-Won Yoon , Kwang-Mahn Kim , Jae-Sung Kwon\",\"doi\":\"10.1016/j.jds.2023.10.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background/purpose</h3><p>The retrograde filling material, particularly mineral trioxide aggregate (MTA) employed in apicoectomy, should possess high antibacterial efficacy and osteogenic potential. We evaluated the antibacterial efficacy, biocompatibility, and osteogenic potential following the addition of silver nanoparticles (AgNPs) and calcium fluoride (CaF<sub>2</sub>) in retrograde filling material of MTA.</p></div><div><h3>Materials and methods</h3><p>MTA was mixed with four different solvents. Group 1 (G1): distilled water, Group 2 (G2): 50 ppm AgNPs, Group 3 (G3): 1 wt% CaF<sub>2</sub>, and Group 4 (G4): 50 ppm AgNPs and 1 wt% CaF<sub>2</sub>. The pH variation of each group was monitored, while the surface roughness was measured. The antibacterial efficacy against <em>Enterococcus faecalis (E. faecalis)</em> and the viability of murine pre-osteoblast (MC3T3) were evaluated for each group using colorimetric assays. The gene expression levels of osteogenic potential marker (OCN, ALPL, and RUNX2) in MC3T3 cells for each group were quantified using real-time-qPCR. Statistical analysis was performed at α = 0.05 level of significance.</p></div><div><h3>Results</h3><p>When comparing the levels of antibacterial efficacy, the order of effectiveness was G4>G2>G3>G1 (<em>P</em> < 0.05). In the cell viability test, owing to MTA-eluted growth medium having a positive effect on MC3T3 cell proliferation, G1–4 exhibited a statistically increased cell viability compared to the control (<em>P</em> < 0.05). However, G2–4 did not result in a statistically significant difference when compared to G1 (<em>P</em> < 0.05). Moreover, G4 exhibited the highest gene expression among the four groups (<em>P</em> < 0.05).</p></div><div><h3>Conclusion</h3><p>The addition of AgNPs and CaF<sub>2</sub> to MTA could be a promising option for use as a new retrograde filling material.</p></div>\",\"PeriodicalId\":15583,\"journal\":{\"name\":\"Journal of Dental Sciences\",\"volume\":\"19 3\",\"pages\":\"Pages 1783-1791\"},\"PeriodicalIF\":3.4000,\"publicationDate\":\"2024-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1991790223003367/pdfft?md5=ff4fa48d97abd875da151f98409b0f6a&pid=1-s2.0-S1991790223003367-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Dental Sciences\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1991790223003367\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Dental Sciences","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1991790223003367","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Antibacterial efficacy and osteogenic potential of mineral trioxide aggregate-based retrograde filling material incorporated with silver nanoparticle and calcium fluoride
Background/purpose
The retrograde filling material, particularly mineral trioxide aggregate (MTA) employed in apicoectomy, should possess high antibacterial efficacy and osteogenic potential. We evaluated the antibacterial efficacy, biocompatibility, and osteogenic potential following the addition of silver nanoparticles (AgNPs) and calcium fluoride (CaF2) in retrograde filling material of MTA.
Materials and methods
MTA was mixed with four different solvents. Group 1 (G1): distilled water, Group 2 (G2): 50 ppm AgNPs, Group 3 (G3): 1 wt% CaF2, and Group 4 (G4): 50 ppm AgNPs and 1 wt% CaF2. The pH variation of each group was monitored, while the surface roughness was measured. The antibacterial efficacy against Enterococcus faecalis (E. faecalis) and the viability of murine pre-osteoblast (MC3T3) were evaluated for each group using colorimetric assays. The gene expression levels of osteogenic potential marker (OCN, ALPL, and RUNX2) in MC3T3 cells for each group were quantified using real-time-qPCR. Statistical analysis was performed at α = 0.05 level of significance.
Results
When comparing the levels of antibacterial efficacy, the order of effectiveness was G4>G2>G3>G1 (P < 0.05). In the cell viability test, owing to MTA-eluted growth medium having a positive effect on MC3T3 cell proliferation, G1–4 exhibited a statistically increased cell viability compared to the control (P < 0.05). However, G2–4 did not result in a statistically significant difference when compared to G1 (P < 0.05). Moreover, G4 exhibited the highest gene expression among the four groups (P < 0.05).
Conclusion
The addition of AgNPs and CaF2 to MTA could be a promising option for use as a new retrograde filling material.
期刊介绍：
he Journal of Dental Sciences (JDS), published quarterly, is the official and open access publication of the Association for Dental Sciences of the Republic of China (ADS-ROC). The precedent journal of the JDS is the Chinese Dental Journal (CDJ) which had already been covered by MEDLINE in 1988. As the CDJ continued to prove its importance in the region, the ADS-ROC decided to move to the international community by publishing an English journal. Hence, the birth of the JDS in 2006. The JDS is indexed in the SCI Expanded since 2008. It is also indexed in Scopus, and EMCare, ScienceDirect, SIIC Data Bases.
The topics covered by the JDS include all fields of basic and clinical dentistry. Some manuscripts focusing on the study of certain endemic diseases such as dental caries and periodontal diseases in particular regions of any country as well as oral pre-cancers, oral cancers, and oral submucous fibrosis related to betel nut chewing habit are also considered for publication. Besides, the JDS also publishes articles about the efficacy of a new treatment modality on oral verrucous hyperplasia or early oral squamous cell carcinoma.
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