Sex Determination using Primary and Permanent Teeth under Different Environment Conditions: A Deoxyribonucleic Acid-based Study

Abstract Aims The purpose of this study was to determine the sex of primary and permanent tooth pulp tissue under different environmental conditions using deoxyribonucleic acid (DNA) analysis and the polymerase chain reaction (PCR) amplification method. Materials and Methods For this study, 40 permanent and primary teeth (20 males and 20 females) that were scheduled for extraction were used. For 6 months, the teeth were divided into four groups of ten (5 males and 5 females) based on different environmental conditions. The pulp was extracted from each tooth, DNA was isolated using QIAGEN’s DNeasy Blood and Tissue Kit, and sex was determined using a PCR technique. Results DNA was extracted from all 40 samples subjected to various environmental conditions, but sex was correctly determined in only 90% of the samples. Conclusions PCR-based sex determination using primary and permanent teeth under different environmental conditions for up to 6 months is a reliable technique with high accuracy.