利用核磁共振代谢组学技术评估以60%豆粕为基础的饲料喂养红颡鱼幼鱼营养失衡的肝脏生物标志物

IF 6.3
Fabio Casu , Aaron M. Watson , Justin Yost , T. Gibson Gaylord , Daniel W. Bearden , Michael R. Denson
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引用次数: 0

摘要

采用高豆粕(SBM)饲料喂养红颡鱼幼鱼12周,研究其营养失衡的生物标志物n -甲氨基- l -谷氨酸(FIGLU)。试验了3种无鱼粉、60%豆粕颗粒饲料(分别命名为B12、Fol和Met),以评估在基础添加水平上分别添加维生素B12 (0.012 mg/kg)、叶酸(10 mg/kg)和蛋氨酸(1 g/kg)对生长性能和组织代谢产物的影响。第四种以sbm为基础的日粮(称为B12/Fol/Met)是由B12、叶酸和蛋氨酸的组合配制而成的,以达到上述目标浓度。此外,还试验了添加蛋氨酸(比基础添加水平高出1 g/kg)、富含牛磺酸、赖氨酸和苏氨酸以及矿物质的60% SBM饲粮(FWS)。这种饲料含有配方目标物和添加剂,可以用植物蛋白代替虹鳟鱼饲料中的鱼粉。对照日粮包括以鱼粉为基础的日粮(FM)、不添加基础60%鱼粉的日粮(SBM60)和由同等比例的鱼(雪茄鲦鱼)、鱿鱼和虾组成的“自然”日粮(N),作为生长性能的正参考。配方饲料的总粗蛋白质含量约为37%,总粗脂肪含量约为14%,能量平衡。测量标准生长性能指标,并在第12周收集组织(肝脏、肌肉),使用基于核磁共振(NMR)的代谢组学来评估饮食诱导的代谢变化。结果表明,在所有性能指标上,FWS饲粮优于所有其他SBM饲粮和FM饲粮。0.05)。饲喂N饲料的鱼未检测到FIGLU,但饲喂SBM和FM饲料的鱼均检测到FIGLU。饲喂FWS日粮和Met日粮的鱼肝脏中FIGLU水平低于其他sbm日粮(P <0.05),这表明在不同的补充方案中,蛋氨酸的补充与较低的FIGLU水平相关。FWS日粮产生的组织代谢物谱与饲喂N日粮的鱼更相似。综上所述,FWS饲粮是一种很有前景的以sbm为基础的红颡鱼替代鱼粉饲粮。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of a hepatic biomarker of nutritional imbalance in juvenile red drum (Sciaenops ocellatus) fed 60% soybean meal-based diets using NMR-based metabolomics

A 12-week feeding trial with juvenile red drum (Sciaenops ocellatus) fed high-soybean meal (SBM) diets was conducted to investigate a putative biomarker of nutritional imbalance, N-formimino-L-glutamate (FIGLU). Three fishmeal-free, 60% SBM pelleted diets (named B12, Fol, and Met, respectively) were tested to evaluate the effects on growth performance and tissue metabolite profiles of supplementation of vitamin B12 (0.012 mg/kg), folate (10 mg/kg), methionine (1 g/kg) respectively, above basal supplementation levels. A fourth SBM-based diet (named B12/Fol/Met) was formulated with a combination of B12, folate, and methionine to attain the above-mentioned target concentrations. A fifth 60% SBM diet (named FWS) with methionine supplementation (1 g/kg above basal supplementation levels), enriched with taurine, lysine and threonine as well as minerals, was also tested. This diet contained formulation targets and additives which have allowed for replacing fishmeal with plant proteins in rainbow trout feeds. Control diets included a fishmeal-based diet (named FM), an unsupplemented basal 60% SBM diet (named SBM60), and a “natural” diet (named N) made up of equal parts of fish (cigar minnows), squid and shrimp as a positive reference for growth performance. Formulated feeds contained approximately 37% total crude protein, approximately 14% total crude lipid and were energetically balanced. Standard growth performance metrics were measured, and tissues (liver, muscle) were collected at week 12 to evaluate diet-induced metabolic changes using nuclear magnetic resonance (NMR)-based metabolomics. Our results show that the FWS diet outperformed all other SBM diets and the FM diet under all performance metrics (P < 0.05). FIGLU was not detected in fish fed the N diet but was detected in those fed the SBM diets and the FM diet. Fish fed the FWS diet and the Met diet showed lower hepatic levels of FIGLU compared with the other SBM-based diets (P < 0.05), suggesting that among the different supplementation regimes, methionine supplementation was associated with lower FIGLU levels. The FWS diet produced tissue metabolite profiles that were more similar to those of fish fed the N diet. Based on our results, the FWS diet constitutes a promising SBM-based alternative diet to fishmeal for red drum.

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来源期刊
Animal Nutrition
Animal Nutrition Animal Science and Zoology
CiteScore
9.70
自引率
0.00%
发文量
542
审稿时长
65 days
期刊介绍: Animal Nutrition encompasses the full gamut of animal nutritional sciences and reviews including, but not limited to, fundamental aspects of animal nutrition such as nutritional requirements, metabolic studies, body composition, energetics, immunology, neuroscience, microbiology, genetics and molecular and cell biology related to primarily to the nutrition of farm animals and aquatic species. More applied aspects of animal nutrition, such as the evaluation of novel ingredients, feed additives and feed safety will also be considered but it is expected that such studies will have a strong nutritional focus. Animal Nutrition is indexed in SCIE, PubMed Central, Scopus, DOAJ, etc.
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