A Review on Nano/Microfluidic Devices for Cell Isolation Techniques: Recent Progress and Advances

Abstract: Micro/nanofluidic devices and systems have gained increasing interest in healthcare applications over the last few decades because of their low cost and ease of customization, with only a small volume of sample fluid required. Many biological queries are now being addressed using various types of single-molecule research. With this rapid rise, the disadvantages of these methods are also becoming obvious. Micro/nanofluidics-based biochemical analysis outperforms traditional approaches in terms of sample volume, turnaround time, ease of operation, and processing efficiency. A complex and multifunctional micro/nanofluidic platform may be used for single-cell manipulation, treatment, detection, and sequencing. We present an overview of the current advances in micro/nanofluidic technology for single-cell research, focusing on cell capture, treatment, and biochemical analyses. The promise of single-cell analysis using micro/nanofluidics is also highlighted.